Anti-Oct4 antibody ab19857 is a rabbit polyclonal antibody that is used in Oct4 western blotting and immunofluorescence. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2005
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- Nature communications 14:76192023Mid-old cells are a potential target for anti-aging interventions in the elderly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung Hwa Kim,Young-Kyoung Lee,Soon Sang Park,So Hyun Park,So Yeong Eom,Young-Sam Lee,Wonhee John Lee,Juhee Jang,Daeha Seo,Hee Young Kang,Jin Cheol Kim,Su Bin Lim,Gyesoon Yoon,Hong Seok Kim,Jang-Hee Kim,Tae Jun ParkPubMed 37993434
- Heliyon 9:e225862023SOX8 promotes tumor growth and metastasis through FZD6-dependent Wnt/β-catenin signaling in colorectal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Li,Boran Cheng,Xiaodong Yang,Gangling Tong,Fen Wang,Mengqing Li,Xiangyu Wang,Shubin WangPubMed 38046159
- Stem cells international 2023:68455712023Photobiomodulation Can Enhance Stem Cell Viability in Cochlea with Auditory Neuropathy but Does Not Restore Hearing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSo-Young Chang,Eunjeong Kim,Nathaniel T Carpena,Jae-Hun Lee,Doo Hee Kim,Min Young LeePubMed 38020205
- Scientific data 10:7552023Profiling mitochondria-polyribosome lncRNAs associated with pluripotency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Zhou,Hui Li,Tingge Sun,Xue Wen,Chao Niu,Min Li,Wei Li,Miguel A Esteban,Andrew R Hoffman,Ji-Fan Hu,Jiuwei CuiPubMed 37919270
- International journal of molecular sciences 24:2023Regulatory Elements Outside Established Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronika V Ermakova,Nikita P Fokin,Nikolay D Aksenov,Evgeny I Bakhmet,Ekaterina V Aleksandrova,Andrey A Kuzmin,Alexey N TomilinPubMed 37895112
- Biological procedures online 25:262023Comparison of two protocols for the generation of iPSC-derived human astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrycja Mulica,Carmen Venegas,Zied Landoulsi,Katja Badanjak,Sylvie Delcambre,Maria Tziortziou,Soraya Hezzaz,Jenny Ghelfi,Semra Smajic,Jens Schwamborn,Rejko Krüger,Paul Antony,Patrick May,Enrico Glaab,Anne Grünewald,Sandro L PereiraPubMed 37730545
- Nature communications 14:53412023Ascl1 and Ngn2 convert mouse embryonic stem cells to neurons via functionally distinct paths.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGintautas Vainorius,Maria Novatchkova,Georg Michlits,Juliane Christina Baar,Cecilia Raupach,Joonsun Lee,Ramesh Yelagandula,Marius Wernig,Ulrich EllingPubMed 37660160
- Metabolites 13:2023Perturbations of Glutathione and Sphingosine Metabolites in Port Wine Birthmark Patient-Derived Induced Pluripotent Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVi Nguyen,Jacob Kravitz,Chao Gao,Marcelo L Hochman,Dehao Meng,Dongbao Chen,Yunguan Wang,Anil G Jegga,J Stuart Nelson,Wenbin TanPubMed 37755263
- International journal of molecular sciences 24:2023A Novel Genetic Variant in Associated with Severe Epilepsy and Intellectual Disability: Potential Implications on Neural Primary Cilia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Martins,Ana Rafaela Oliveira,Solange Martins,José Pedro Vieira,Pedro Perdigão,Ana Rita Fernandes,Luís Pereira de Almeida,Paulo Jorge Palma,Diana Bela Sequeira,João Miguel Marques Santos,Frederico Duque,Guiomar Oliveira,Ana Luísa Cardoso,João Peça,Catarina Morais SeabraPubMed 37628781
- Nature communications 14:41092023Gain and loss of function variants in EZH1 disrupt neurogenesis and cause dominant and recessive neurodevelopmental disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarolina Gracia-Diaz,Yijing Zhou,Qian Yang,Reza Maroofian,Paula Espana-Bonilla,Chul-Hwan Lee,Shuo Zhang,Natàlia Padilla,Raquel Fueyo,Elisa A Waxman,Sunyimeng Lei,Garrett Otrimski,Dong Li,Sarah E Sheppard,Paul Mark,Margaret H Harr,Hakon Hakonarson,Lance Rodan,Adam Jackson,Pradeep Vasudevan,Corrina Powel,Shehla Mohammed,Sateesh Maddirevula,Hamad Alzaidan,Eissa A Faqeih,Stephanie Efthymiou,Valentina Turchetti,Fatima Rahman,Shazia Maqbool,Vincenzo Salpietro,Shahnaz H Ibrahim,Gabriella di Rosa,Henry Houlden,Maha Nasser Alharbi,Nouriya Abbas Al-Sannaa,Peter Bauer,Giovanni Zifarelli,Conchi Estaras,Anna C E Hurst,Michelle L Thompson,Anna Chassevent,Constance L Smith-Hicks,Xavier de la Cruz,Alexander M Holtz,Houda Zghal Elloumi,M J Hajianpour,Claudine Rieubland,Dominique Braun,Siddharth Banka,Deborah L French,Elizabeth A Heller,Murielle Saade,Hongjun Song,Guo-Li Ming,Fowzan S Alkuraya,Pankaj B Agrawal,Danny Reinberg,Elizabeth J Bhoj,Marian A Martínez-Balbás,Naiara AkizuPubMed 37433783
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Tested | Tested |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-5.00000 µg/mL | Notes - |
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Alternative names
OCT3, OCT4, OTF3, OCT3, POU5F1, OCT4, OTF3, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3
Recommended products
Anti-Oct4 antibody ab19857 is a rabbit polyclonal antibody that is used in Oct4 western blotting and immunofluorescence. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2005
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Protein A
- Specificity
Previous batches of ab19857 have worked well in IHC, but recent batches of this antibody are showing non-specific staining on day 6 mouse testis in our lab (as of Nov 2016).
Replenishment batches of our polyclonal antibody, ab19857 are tested in WB. Previous batches were additionally validated in ICC/IF and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab181557.
- Concentration
- Purification notes
Affinity Purified
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
- Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
- Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
- Associated diseases and disorders
Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
Immunoprecipitation - Anti-Oct4 antibody (ab19857)
Oct4 was immunoprecipitated using 0.5mg E14Tg2a whole cell extract, 5μg of Rabbit polyclonal to Oct4 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, E14Tg2a whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19857.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 48kDa: Oct4.All lanes: Immunoprecipitation - Anti-Oct4 antibody (ab19857)
Predicted band size: 38 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab19857)
ab19857 staining Oct4 in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab19857 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Western blot - Anti-Oct4 antibody (ab19857)
Anti-Oct4 antibody ab19857 only detected a band corresponding to the expected size of Oct-4 in human ES cell lysate. In mouse ES and EG cell lysates, ab19857 detected a band of approximately 55 kDa in addition to the expected 39 kDa Oct-4 band.
All lanes: Western blot - Anti-Oct4 antibody (ab19857) at 1 µg/mL
Lanes 1 and 4: Human embryonic stem cell lysate at 20 µg
Lanes 2 and 5: Mouse embryonic stem cell lysate at 20 µg
Lanes 3 and 6: Mouse embryonic germ cell lysate at 20 µg
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab19857)
ICC/IF image of ab19857 stained Mouse Embryonic Stem cells. The cells were PFA fixed (4% PFA, 20 min) and incubated with the antibody (ab19857, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT™ FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue).
The large nuclei of the Feeder cells can be seen in the image; ab19857 does not localise to these nuclei. ab19857 can be seen localising to the much smaller nuclei of the Mouse Embryonic Stem cells.
Western blot - Anti-Oct4 antibody (ab19857)
Anti-Oct4 antibody ab19857 only detected a band corresponding to the expected size of Oct4 in human ES cell lysate. In mouse ES and EG cell lysates, ab19857 detected a band of approximately 55 kDa in addition to the expected 39 kDa Oct4 band.
All lanes: Western blot - Anti-Oct4 antibody (ab19857) at 1 µg/mL
Lanes 1 and 4: Human embryonic stem cell lysate at 20 µg
Lanes 2 and 5: Mouse embryonic stem cell lysate at 20 µg
Lanes 3 and 6: Mouse embryonic germ cell lysate at 20 µg
Predicted band size: 38 kDa
Observed band size: 43 kDa, 55 kDa
This image is courtesy of Ludovic Vallier, University of CambridgeImmunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab19857)
The image shows a colony of differentiating Human Embryonic Stem Cells double-stained with anti-Oct4 antibody ab19857 (green) and Sox17 antibody (red). The nuclei of Oct-4-positive undifferentiated hESCs stained bright green. Staining was restricted to the nuclei. Oct4-positive nuclei were Sox17-negative, and vice versa. This antibody can be used as a marker of Oct4-positive undifferentiated Human Embryonic Stem Cells.
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab19857)
ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid (trans-Retinoic acid, Stem cell differentiator ab120728) The cells were incubated at 37°C for 2 days in media containing different concentrations of trans-Retinoic acid, Stem cell differentiator ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19857 (1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1:250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Western blot - Anti-Oct4 antibody (ab19857)
MEL-1 and MEL-2 Human Embryonic Stem Cell Lysates (ab27198 and ab27196), IOUD2 Oct4-expressing Mouse Embryonic Stem Cell Lysate (ab27202) and F9 Mouse Embryonic Carcinoma Cell Lysate (ab27193) all contain Oct4 protein. As expected, a band corresponding to Oct4 was detected in all four of the lysates by Westen Blot using anti-Oct4 antibody ab19857. Each of the Oct4 bands was specifically blocked using the immunising peptide of ab19857. The origin of the additional 65 kDa band detected in Lane 3 is unknown and may be a non-specific band that is recognised, in addition to Oct4, by ab19857 in mouse embryonic stem cells.
All lanes: Western blot - Anti-Oct4 antibody (ab19857) at 1 µg/mL
Lanes 1 and 5: MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198) at 10 µL
Lanes 2 and 6: MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µL
Lanes 3 and 7: IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate (ab27202) at 10 µL
Lanes 4 and 8: F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193) at 10 µL
Predicted band size: 38 kDa
Observed band size: 48 kDa, 65 kDa
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