JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB184665

Anti-Oct4 antibody [GT486]

5

(2 Reviews)

|

(46 Publications)

Anti-Oct4 antibody [GT486] (ab184665) is a mouse monoclonal antibody detecting Oct4 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Over 30 publications

View Alternative Names

OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3

8 Images
Immunoprecipitation - Anti-Oct4 antibody [GT486] (AB184665)
  • IP

Supplier Data

Immunoprecipitation - Anti-Oct4 antibody [GT486] (AB184665)

Immunoprecipitation of Oct4 transfected 293T cell lysate followed by detection of Oct4 with ab184665 at 1/1000 dilution.
Lane 1; Cell lysate/extract of transfected 293T cells.

Lane 2; Cell lysate/extract of transfected 293T cells precipitated with 2 μg of preimmune mouse IgG (control)

Lane 3; Cell lysate/extract of transfected 293T cells precipitated using 2 μg of ab184665.

Detection utilised anti-mouse IgG. Samples analysed by 12% SDS-PAGE.

All lanes:

Immunoprecipitation - Anti-Oct4 antibody [GT486] (ab184665)

Predicted band size: 38 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (AB184665)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (AB184665)

Immunofluorescence analysis of 4% paraformaldehyde-fixed Human embryonic stem cells labeling Oct4 with ab184665 at 1/200 dilution (green). Hoechst 33342 staining is shown in blue.

Flow Cytometry - Anti-Oct4 antibody [GT486] (AB184665)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Oct4 antibody [GT486] (AB184665)

Flow cytometric analysis of Human embryonic stem cells labeling Oct4 with ab184665 at 1/50 dilution (green). The isotype control was at 1/50 dilution (Black).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (AB184665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (AB184665)

Immunohistochemical analysis of paraffin-embedded BT483 (human) xenograft tissue labeling Oct4 with ab184665 at 1/200 dilution.

Western blot - Anti-Oct4 antibody [GT486] (AB184665)
  • WB

Supplier Data

Western blot - Anti-Oct4 antibody [GT486] (AB184665)

10% SDS PAGE

All lanes:

Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution

Lane 1:

Non-transfected HeLa whole cell lysates at 10 µg

Lane 2:

OCT3/4 transfected HeLa whole cell lysates at 10 µg

Predicted band size: 38 kDa

false

Western blot - Anti-Oct4 antibody [GT486] (AB184665)
  • WB

Supplier Data

Western blot - Anti-Oct4 antibody [GT486] (AB184665)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution

All lanes:

Human ESC whole cell lysate/extract at 30 µg

Predicted band size: 38 kDa

false

Western blot - Anti-Oct4 antibody [GT486] (AB184665)
  • WB

Supplier Data

Western blot - Anti-Oct4 antibody [GT486] (AB184665)

10% SDS PAGE

All lanes:

Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution

All lanes:

Mouse ESC whole cell lysate at 20 µg

Predicted band size: 38 kDa

false

Western blot - Anti-Oct4 antibody [GT486] (AB184665)
  • WB

Supplier Data

Western blot - Anti-Oct4 antibody [GT486] (AB184665)

10% SDS PAGE

All lanes:

Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution

All lanes:

NT2D1 whole cell lysate at 30 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT486

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human POU5F1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q01860

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

What is this antibody validated in?
Anti-Oct4 antibody [GT486] (ab184665) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of Oct4?
Anti-Oct4 [GT486] (ab184665) specifically detects a band for Oct4 (UniProt: Q01860) at a molecular weight of 39kDa.

Trusted by the scientific community
Anti-Oct4 [GT486] (ab184665) was first used in a scientific publication in 2014 and has been cited over 30 times in peer-reviewed journals.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS
Storage buffer
pH: 7 Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
Biological function summary

Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.

Pathways

Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.

Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The protein expressed by the gene POU5F1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and forms a trimeric complex with SOX2 or SOX15 on DNA. It controls the expression of several genes involved in embryonic development, including YES1, FGF4, UTF1, and ZFP206. This protein is critical for early embryogenesis and for maintaining embryonic stem cell pluripotency. This supplementary information is collated from multiple sources and compiled automatically.
See full target information POU5F1
style
left-column

Publications (46)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39596521

2024

The Generation of Genetically Engineered Human Induced Pluripotent Stem Cells Overexpressing IFN-β for Future Experimental and Clinically Oriented Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Olga Sheveleva,Elena Protasova,Elena Grigor'eva,Nina Butorina,Valeriia Kuziaeva,Daniil Antonov,Victoria Melnikova,Sergey Medvedev,Irina Lyadova

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2407757 PubMed39587837

2024

Self-Healing COCu-Tac Hydrogel Enhances iNSCs Transplantation for Spinal Cord Injury by Promoting Mitophagy via the FKBP52/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenming Tian,Han-Jian Hu,Chun Cheung Chan,Tian Hu,Chaoyang Cai,Hong Li,Limin Rong,Gang-Biao Jiang,Bin Liu

PloS one 19:e0311565 PubMed39485792

2024

Generation of isogenic models of Angelman syndrome and Prader-Willi syndrome in CRISPR/Cas9-engineered human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel B Gilmore,Dea Gorka,Christopher E Stoddard,Pooja Sonawane,Justin Cotney,Stormy J Chamberlain

Nature communications 15:2859 PubMed38570500

2024

Cold-induced FOXO1 nuclear transport aids cold survival and tissue storage.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomei Zhang,Lihao Ge,Guanghui Jin,Yasong Liu,Qingfen Yu,Weizhao Chen,Liang Chen,Tao Dong,Kiyoharu J Miyagishima,Juan Shen,Jinghong Yang,Guo Lv,Yan Xu,Qing Yang,Linsen Ye,Shuhong Yi,Hua Li,Qi Zhang,Guihua Chen,Wei Liu,Yang Yang,Wei Li,Jingxing Ou

Cancers 16: PubMed38398205

2024

Transduction Efficiency of Zika Virus E Protein Pseudotyped HIV-1 and Its Oncolytic Activity Tested in Primary Glioblastoma Cell Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Patrick Formanski,Hai Dang Ngo,Vivien Grunwald,Celine Pöhlking,Jana Sue Jonas,Dominik Wohlers,Birco Schwalbe,Michael Schreiber

International journal of molecular sciences 24: PubMed37833934

2023

Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vivien Grunwald,Hai Dang Ngo,Jan Patrick Formanski,Jana Sue Jonas,Celine Pöhlking,Birco Schwalbe,Michael Schreiber

Neoplasma 70:430-442 PubMed37498061

2023

Circular RNA_0000326 accelerates breast cancer development via modulation of the miR-9-3p/YAP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Ling Xue,Shuai Zhao,Meng-Chang Xu,Ying Li,Wen-Fei Liu,Hong-Zhen Qin

CNS neuroscience & therapeutics 29:3756-3773 PubMed37309294

2023

Exosomal miR-6733-5p mediates cross-talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically.

Applications

Unspecified application

Species

Unspecified reactive species

Shilu Huang,Liang Liu,Zhipeng Xu,Xinglei Liu,Anyi Wu,Xiaopei Zhang,Zengyang Li,Suwen Li,Yongdong Li,Jiaqi Yuan,Shan Cheng,Haoran Li,Jun Dong

Pharmaceuticals (Basel, Switzerland) 16: PubMed37111381

2023

rAAV2-Mediated Restoration of GALC in Neural Stem Cells from Krabbe Patient-Derived iPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Guoshuai Tian,Chunyu Cao,Shuyue Li,Wei Wang,Ye Zhang,Yafeng Lv

American journal of cancer research 13:1471-1485 PubMed37168326

2023

Somatic-to-primordial germ cell-like transformation is critical in tumor initiation of mouse breast tumor 4T1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Ma,Fengyu Zhang,Aiping Liu,Huikuan Lin,Chunfang Liu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB208907

Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Oct4 antibody [EPR17980] (AB208907)

  • 0
  • 0
Primary Antibodies

AB213198

PE Anti-Oct4 antibody [EPR17980]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-Oct4 antibody [EPR17980] (AB213198)

  • 0
  • 0
Primary Antibodies

AB109183

Anti-Oct4 antibody [EPR2054]

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (AB109183)

  • 5
  • 2
Primary Antibodies

AB181557

Anti-Oct4 antibody [EPR17929] - ChIP Grade

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [EPR17929] - ChIP Grade (AB181557)

  • 5
  • 20
Primary Antibodies

AB200834

Anti-Oct4 antibody [EPR17980]

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR17980] (AB200834)

  • 5
  • 4

Complementary Products

Assay Kits

AB235653

Human/Mouse Oct4 ELISA Kit

Recombinant|SimpleStep

ELISA - Human/Mouse Oct4 ELISA Kit (AB235653)

  • 0
  • 0
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75
Primary Antibodies

AB109250

Anti-Nanog antibody [EPR2027(2)]

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)] (AB109250)

  • 4
  • 11
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16287

Anti-SSEA4 antibody [MC813-70]

Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

  • 4
  • 11

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com