Mouse Monoclonal Oct4 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 32 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU5F1 aa 1-200.
Images
![Immunoprecipitation - Anti-Oct4 antibody [GT486] (AB184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--immunoprecipitation-img86671.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Oct4 antibody [GT486] (AB184665)")
![Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (AB184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--immunocytochemistry-immunofluorescence-img97036.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (AB184665)")
![Western blot - Anti-Oct4 antibody [GT486] (AB184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img96740.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oct4 antibody [GT486] (AB184665)")
![Flow Cytometry - Anti-Oct4 antibody [GT486] (AB184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--flow-cytometry-img89103.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Oct4 antibody [GT486] (AB184665)")
![Western blot - Anti-Oct4 antibody [GT486] (AB184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img96566.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oct4 antibody [GT486] (AB184665)")
Publications
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- International journal of molecular sciences 24:2023Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVivien Grunwald,Hai Dang Ngo,Jan Patrick Formanski,Jana Sue Jonas,Celine Pöhlking,Birco Schwalbe,Michael SchreiberPubMed 37833934
- Pharmaceuticals (Basel, Switzerland) 16:2023rAAV2-Mediated Restoration of GALC in Neural Stem Cells from Krabbe Patient-Derived iPSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoshuai Tian,Chunyu Cao,Shuyue Li,Wei Wang,Ye Zhang,Yafeng LvPubMed 37111381
- American journal of cancer research 13:1471-14852023Somatic-to-primordial germ cell-like transformation is critical in tumor initiation of mouse breast tumor 4T1 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhan Ma,Fengyu Zhang,Aiping Liu,Huikuan Lin,Chunfang LiuPubMed 37168326
- Journal of ovarian research 16:762023Chito-oligosaccharides and macrophages have synergistic effects on improving ovarian stem cells function by regulating inflammatory factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Zheng,Wenli Hong,Haifeng Ye,Ziqiong Zhou,Shuyi Ling,Yuan Li,Yuqing Dai,Zhisheng Zhong,Ziwei Yang,Yuehui ZhengPubMed 37060101
- Cancer cell international 22:3712022Activation of embryonic/germ cell-like axis links poor outcomes of gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhan Ma,Fengyu Zhang,Ji Xiong,Haishi Zhang,Hui-Kuan Lin,Chunfang LiuPubMed 36435765
- Cancers 14:2022Stage-Specific Effect of Inositol Hexaphosphate on Cancer Stem Cell Pool during Growth and Progression of Prostate Tumorigenesis in TRAMP Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKomal Raina,Kushal Kandhari,Anil K Jain,Kameswaran Ravichandran,Paul Maroni,Chapla Agarwal,Rajesh AgarwalPubMed 36077751
- Frontiers in nutrition 9:8546552022Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianyu Zhang,Zhifu Wei,Huinan Weng,Ye Chen,Jie Zhang,Shiwei Mei,Jiahui Wei,Xiulan Zhu,Yingqi Nong,Jianxing Ruan,Wenjuan Liu,Ruiqiong Zhou,Fang Wang,Yanni Xie,Junjiu Huang,Xiqian Zhang,Fenghua LiuPubMed 35836584
- Science advances 8:eabl80702022Depletion of oocyte dynamin-related protein 1 shows maternal-effect abnormalities in embryonic development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepak Adhikari,In-Won Lee,Usama Al-Zubaidi,Jun Liu,Qing-Hua Zhang,Wai Shan Yuen,Likun He,Yasmyn Winstanley,Hiromi Sesaki,Jeffrey R Mann,Rebecca L Robker,John CarrollPubMed 35704569
- Nucleic acids research 50:5313-53342022Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesW Samuel Fagg,Naiyou Liu,Ulrich Braunschweig,Karen Larissa Pereira de Castro,Xiaoting Chen,Frederick S Ditmars,Steven G Widen,John Paul Donohue,Katalin Modis,William K Russell,Jeffrey H Fair,Matthew T Weirauch,Benjamin J Blencowe,Mariano A Garcia-BlancoPubMed 35544276
- Cell biochemistry and function 40:359-3682022The porcupine inhibitor WNT974 provokes ectodermal lineage differentiation of human embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshkan Kalantary-Charvadeh,Vahid Hosseini,Amir Mehdizadeh,Saeed Nazari Soltan Ahmad,Reza Rahbarghazi,Hojjatollah Nozad Charoudeh,Mohammad Nouri,Masoud DarabiPubMed 35445405
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human POU5F1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q01860
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Alternative names
OCT3, OCT4, OTF3, POU5F1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, Oct-3, Oct-4, OTF-3
Recommended products
Mouse Monoclonal Oct4 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 32 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU5F1 aa 1-200.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human POU5F1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q01860
- Clone number
- GT486
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from TCS
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 17th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Oct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.
- Biological function summary
Oct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.
- Pathways
Oct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.
- Associated diseases and disorders
Aberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
![Immunoprecipitation - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--immunoprecipitation-img86671.jpg "Immunoprecipitation - Anti-Oct4 antibody [GT486] (ab184665)")
Immunoprecipitation - Anti-Oct4 antibody [GT486] (ab184665)
Immunoprecipitation of Oct4 transfected 293T cell lysate followed by detection of Oct4 with ab184665 at 1/1000 dilution.
Lane 1; Cell lysate/extract of transfected 293T cells.Lane 2; Cell lysate/extract of transfected 293T cells precipitated with 2 μg of preimmune mouse IgG (control)
Lane 3; Cell lysate/extract of transfected 293T cells precipitated using 2 μg of ab184665.
Detection utilised anti-mouse IgG. Samples analysed by 12% SDS-PAGE.All lanes: Immunoprecipitation - Anti-Oct4 antibody [GT486] (ab184665)
Predicted band size: 38 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--immunocytochemistry-immunofluorescence-img97036.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (ab184665)")
Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody [GT486] (ab184665)
Immunofluorescence analysis of 4% paraformaldehyde-fixed Human embryonic stem cells labeling Oct4 with ab184665 at 1/200 dilution (green). Hoechst 33342 staining is shown in blue.
![Western blot - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img96740.jpg "Western blot - Anti-Oct4 antibody [GT486] (ab184665)")
Western blot - Anti-Oct4 antibody [GT486] (ab184665)
10% SDS PAGE
All lanes: Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution
All lanes: Mouse ESC whole cell lysate at 20 µg
Predicted band size: 38 kDa
![Flow Cytometry - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--flow-cytometry-img89103.jpg "Flow Cytometry - Anti-Oct4 antibody [GT486] (ab184665)")
Flow Cytometry - Anti-Oct4 antibody [GT486] (ab184665)
Flow cytometric analysis of Human embryonic stem cells labeling Oct4 with ab184665 at 1/50 dilution (green). The isotype control was at 1/50 dilution (Black).
![Western blot - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img96566.jpg "Western blot - Anti-Oct4 antibody [GT486] (ab184665)")
Western blot - Anti-Oct4 antibody [GT486] (ab184665)
10% SDS PAGE
All lanes: Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution
All lanes: NT2D1 whole cell lysate at 30 µg
Predicted band size: 38 kDa
![Western blot - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img155849.jpg "Western blot - Anti-Oct4 antibody [GT486] (ab184665)")
Western blot - Anti-Oct4 antibody [GT486] (ab184665)
10% SDS PAGE
All lanes: Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution
Lane 1: Non-transfected HeLa whole cell lysates at 10 µg
Lane 2: OCT3/4 transfected HeLa whole cell lysates at 10 µg
Predicted band size: 38 kDa
![Western blot - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img101918.jpg "Western blot - Anti-Oct4 antibody [GT486] (ab184665)")
Western blot - Anti-Oct4 antibody [GT486] (ab184665)
10 % SDS-PAGE
All lanes: Western blot - Anti-Oct4 antibody [GT486] (ab184665) at 1/1000 dilution
All lanes: Human ESC whole cell lysate/extract at 30 µg
Predicted band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131979.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (ab184665)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (ab184665)
Immunohistochemical analysis of paraffin-embedded BT483 (human) xenograft tissue labeling Oct4 with ab184665 at 1/200 dilution.
![Western blot - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img155849.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oct4 antibody [GT486] (ab184665)")
![Western blot - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--western-blot-img101918.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oct4 antibody [GT486] (ab184665)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (ab184665), expandable thumbnail](https://content.abcam.com/products/images/oct4-antibody-gt486-ab184665--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131979.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [GT486] (ab184665)")
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