AB221964

Anti-Oct6 antibody

Name: Anti-Oct6 antibody (ab221964) | Abcam
Brand: Abcam Limited
SKU: AB221964

(1 Review)

(2 Publications)

Rabbit Polyclonal Oct6 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F1 aa 1-100.

View Alternative Names

OCT6, OTF6, POU3F1, Octamer-binding protein 6, Octamer-binding transcription factor 6, POU domain transcription factor SCIP, Oct-6, OTF-6

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody (AB221964)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody (AB221964)

PFA-fixed, Triton X-100 permeabilized SK-MEL-30 cells stained for Oct6 (green) using ab221964 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human POU3F1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q03052

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct6 also known as Pou3f1 is a transcription factor with a molecular mass of approximately 45 kDa. It functions as a DNA-binding protein that influences gene expression during development. Oct6 belongs to the POU domain transcription factor family which plays a significant role in regulating specific genes across different cellular contexts. This protein is expressed mainly in the nervous system specifically in oligodendrocytes Schwann cells and some sensory organs reflecting its involvement in neural development.

Biological function summary

Oct6 regulates important developmental processes by controlling the timing and progression of myelination in the peripheral nervous system. It is a part of a multi-protein complex that includes other transcription factors necessary for glial cell differentiation. Oct6 acts by promoting the expression of genes essential for the development and function of the myelin sheath thereby maintaining proper nerve conduction and insulation.

Pathways

Oct6 interacts within the myelination process specifically the Sonic Hedgehog signaling and WNT pathways. These pathways govern the regulation of cell growth and differentiation important for neural development. Oct6 also shows interaction with other proteins like Sox10 a fellow transcription factor important for Schwann cell development enhancing its regulatory influence in neurogenesis.

Oct6 links to conditions such as Charcot-Marie-Tooth disease and Dejerine-Sottas syndrome both affecting peripheral nerves. These disorders often result from malfunction in myelination processes which Oct6 directly influences. The protein collaborates with Sox10 and EGR2 another transcription factor vital in myelination thereby implicating it in neuropathic disease mechanisms. Understanding these interactions may help develop therapeutic strategies for demyelinating diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). Acts as a transcriptional repressor of myelin-specific genes (By similarity).

See full target information POU3F1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 16:1009542 PubMed36726852

2023

A three-component model of the spinal nerve ramification: Bringing together the human gross anatomy and modern Embryology.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsaku Homma,Takako Shimada,Ikuo Wada,Katsuji Kumaki,Noboru Sato,Hiroyuki Yaginuma

Cancers 14: PubMed35626145

2022

Angiopoietin-1 Upregulates Cancer Cell Motility in Colorectal Cancer Liver Metastases through Actin-Related Protein 2/3.

Applications

Unspecified application

Species

Unspecified reactive species

Miran Rada,Audrey Kapelanski-Lamoureux,Migmar Tsamchoe,Stephanie Petrillo,Anthoula Lazaris,Peter Metrakos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Oct6 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody (AB221964)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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