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AB272925

Anti-Oct6 antibody

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(7 Publications)

Rabbit Polyclonal Oct6 antibody. Suitable for IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pou3f1.

View Alternative Names

OCT6, OTF6, POU3F1, Octamer-binding protein 6, Octamer-binding transcription factor 6, POU domain transcription factor SCIP, Oct-6, OTF-6

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody (AB272925)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Oct6 antibody (AB272925)

4% PFA-fixed A549 (Human lung carcinoma cell line) cells stained for Oct6 using ab272925 at 1/500 dilution in ICC/IF (green). Red : Phalloidin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody (AB272925)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody (AB272925)

Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272925 at 1/500 dilution (green) in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes. Red : SOX2. Blue : DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody (AB272925)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody (AB272925)

Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272925 at 1/250 dilution (green) in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Red : Beta tubulin.

Blue : DAPI.

Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody (AB272925)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody (AB272925)

Frozen mouse cerebral cortex tissue stained for Oct6 using ab272925 at 1/250 dilution (green) in immunohistochemical analysis. Blue : DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-Fr, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Pou3f1. The exact immunogen used to generate this antibody is proprietary information.

P21952

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Complementary Products

Primary Antibodies

AB155279

Anti-SOX10 antibody [EPR4007]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (AB155279)

  • 4
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Primary Antibodies

AB31940

Anti-TBR1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (AB31940)

  • 4
  • 34
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct6 also known as Pou3f1 is a transcription factor with a molecular mass of approximately 45 kDa. It functions as a DNA-binding protein that influences gene expression during development. Oct6 belongs to the POU domain transcription factor family which plays a significant role in regulating specific genes across different cellular contexts. This protein is expressed mainly in the nervous system specifically in oligodendrocytes Schwann cells and some sensory organs reflecting its involvement in neural development.
Biological function summary

Oct6 regulates important developmental processes by controlling the timing and progression of myelination in the peripheral nervous system. It is a part of a multi-protein complex that includes other transcription factors necessary for glial cell differentiation. Oct6 acts by promoting the expression of genes essential for the development and function of the myelin sheath thereby maintaining proper nerve conduction and insulation.

Pathways

Oct6 interacts within the myelination process specifically the Sonic Hedgehog signaling and WNT pathways. These pathways govern the regulation of cell growth and differentiation important for neural development. Oct6 also shows interaction with other proteins like Sox10 a fellow transcription factor important for Schwann cell development enhancing its regulatory influence in neurogenesis.

Oct6 links to conditions such as Charcot-Marie-Tooth disease and Dejerine-Sottas syndrome both affecting peripheral nerves. These disorders often result from malfunction in myelination processes which Oct6 directly influences. The protein collaborates with Sox10 and EGR2 another transcription factor vital in myelination thereby implicating it in neuropathic disease mechanisms. Understanding these interactions may help develop therapeutic strategies for demyelinating diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). Acts as a transcriptional repressor of myelin-specific genes (By similarity).
See full target information POU3F1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 14:e036377 PubMed40028887

2025

Lactate Dehydrogenase A Crotonylation and Mono-Ubiquitination Maintains Vascular Smooth Muscle Cell Growth and Migration and Promotes Neointima Hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Huan Chen,Shan-Hu Cao,Zhi-Yan Ren,Ting Zhang,Han-Mei Jiang,Zhao-Kun Hu,Li-Hua Dong

International journal of molecular sciences 24: PubMed37895112

2023

Regulatory Elements Outside Established Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika V Ermakova,Nikita P Fokin,Nikolay D Aksenov,Evgeny I Bakhmet,Ekaterina V Aleksandrova,Andrey A Kuzmin,Alexey N Tomilin

STAR protocols 4:102413 PubMed37454299

2023

Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Ciarpella,Raluca Georgiana Zamfir,Alessandra Campanelli,Giulia Pedrotti,Marzia Di Chio,Emanuela Bottani,Ilaria Decimo

Cell reports methods 2:100367 PubMed36590694

2022

-derived medium spiny neurons recapitulate human striatal development and complexity at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Conforti,Vittoria Dickinson Bocchi,Ilaria Campus,Linda Scaramuzza,Maura Galimberti,Tiziana Lischetti,Francesca Talpo,Matteo Pedrazzoli,Alessio Murgia,Ivan Ferrari,Chiara Cordiglieri,Alessandra Fasciani,Ernest Arenas,Dan Felsenfeld,Gerardo Biella,Dario Besusso,Elena Cattaneo

The EMBO journal 41:e108780 PubMed35815410

2022

Schwann cell precursors represent a neural crest-like state with biased multipotency.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Eleni Kastriti,Louis Faure,Dorothea Von Ahsen,Thibault Gerald Bouderlique,Johan Boström,Tatiana Solovieva,Cameron Jackson,Marianne Bronner,Dies Meijer,Saida Hadjab,Francois Lallemend,Alek Erickson,Marketa Kaucka,Viacheslav Dyachuk,Thomas Perlmann,Laura Lahti,Jan Krivanek,Jean-Francois Brunet,Kaj Fried,Igor Adameyko

iScience 24:103438 PubMed34901791

2021

Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Ciarpella,Raluca Georgiana Zamfir,Alessandra Campanelli,Elisa Ren,Giulia Pedrotti,Emanuela Bottani,Andrea Borioli,Davide Caron,Marzia Di Chio,Sissi Dolci,Annika Ahtiainen,Giorgio Malpeli,Giovanni Malerba,Rita Bardoni,Guido Fumagalli,Jari Hyttinen,Francesco Bifari,Gemma Palazzolo,Gabriella Panuccio,Giulia Curia,Ilaria Decimo

Science (New York, N.Y.) 372: PubMed33958447

2021

The coding and long noncoding single-cell atlas of the developing human fetal striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Vittoria Dickinson Bocchi,Paola Conforti,Elena Vezzoli,Dario Besusso,Claudio Cappadona,Tiziana Lischetti,Maura Galimberti,Valeria Ranzani,Raoul J P Bonnal,Marco De Simone,Grazisa Rossetti,Xiaoling He,Kenji Kamimoto,Ira Espuny-Camacho,Andrea Faedo,Federica Gervasoni,Romina Vuono,Samantha A Morris,Jian Chen,Dan Felsenfeld,Giulio Pavesi,Roger A Barker,Massimiliano Pagani,Elena Cattaneo
View all publications
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Product promise

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