AB126746

Anti-Oct6 antibody [EP5421]

Name: Anti-Oct6 antibody [EP5421] (ab126746) | Abcam
Brand: Abcam Limited
SKU: AB126746
Price: 340 USD
Availability: InStock
Rating: 2 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(8 Publications)

Rabbit Recombinant Monoclonal Oct6 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 8 publications.

View Alternative Names

OCT6, OTF6, POU3F1, Octamer-binding protein 6, Octamer-binding transcription factor 6, POU domain transcription factor SCIP, Oct-6, OTF-6

2 Images

Unknown

Western blot - Anti-Oct6 antibody [EP5421] (AB126746)

All lanes:

Western blot - Anti-Oct6 antibody [EP5421] (ab126746) at 1/1000 dilution

Lane 1:

F9 cell lysate at 10 µg

Lane 2:

NCCIT cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 45 kDa

Observed band size: 50 kDa

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OI-RD Scanning - Anti-Oct6 antibody [EP5421] (AB126746)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Oct6 antibody [EP5421] (AB126746)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5421

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oct6 also known as Pou3f1 is a transcription factor with a molecular mass of approximately 45 kDa. It functions as a DNA-binding protein that influences gene expression during development. Oct6 belongs to the POU domain transcription factor family which plays a significant role in regulating specific genes across different cellular contexts. This protein is expressed mainly in the nervous system specifically in oligodendrocytes Schwann cells and some sensory organs reflecting its involvement in neural development.

Biological function summary

Oct6 regulates important developmental processes by controlling the timing and progression of myelination in the peripheral nervous system. It is a part of a multi-protein complex that includes other transcription factors necessary for glial cell differentiation. Oct6 acts by promoting the expression of genes essential for the development and function of the myelin sheath thereby maintaining proper nerve conduction and insulation.

Pathways

Oct6 interacts within the myelination process specifically the Sonic Hedgehog signaling and WNT pathways. These pathways govern the regulation of cell growth and differentiation important for neural development. Oct6 also shows interaction with other proteins like Sox10 a fellow transcription factor important for Schwann cell development enhancing its regulatory influence in neurogenesis.

Oct6 links to conditions such as Charcot-Marie-Tooth disease and Dejerine-Sottas syndrome both affecting peripheral nerves. These disorders often result from malfunction in myelination processes which Oct6 directly influences. The protein collaborates with Sox10 and EGR2 another transcription factor vital in myelination thereby implicating it in neuropathic disease mechanisms. Understanding these interactions may help develop therapeutic strategies for demyelinating diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). Acts as a transcriptional repressor of myelin-specific genes (By similarity).

See full target information POU3F1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed38456457

2024

TEAD1 is crucial for developmental myelination, Remak bundles, and functional regeneration of peripheral nerves.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Grove,Hyukmin Kim,Shuhuan Pang,Jose Paz Amaya,Guoqing Hu,Jiliang Zhou,Michel Lemay,Young-Jin Son

Neuron 111:2184-2200.e7 PubMed37192624

2023

Prox2 and Runx3 vagal sensory neurons regulate esophageal motility.

Applications

Unspecified application

Species

Unspecified reactive species

Elijah D Lowenstein,Pierre-Louis Ruffault,Aristotelis Misios,Kate L Osman,Huimin Li,Rachel S Greenberg,Rebecca Thompson,Kun Song,Stephan Dietrich,Xun Li,Nikita Vladimirov,Andrew Woehler,Jean-François Brunet,Niccolò Zampieri,Ralf Kühn,Stephen D Liberles,Shiqi Jia,Gary R Lewin,Nikolaus Rajewsky,Teresa E Lever,Carmen Birchmeier

Molecular and cellular biology 42:e0055921 PubMed35708320

2022

Aberrant Neuregulin 1/ErbB Signaling in Charcot-Marie-Tooth Type 4D Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ting Jiang,Yu-Hui Chen,Jie-Hong Huang,Wei-Fang Tong,Ling-Jing Jin,Li-Xi Li

Nature 602:129-134 PubMed35082446

2022

Brahma safeguards canalization of cardiac mesoderm differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Swetansu K Hota,Kavitha S Rao,Andrew P Blair,Ali Khalilimeybodi,Kevin M Hu,Reuben Thomas,Kevin So,Vasumathi Kameswaran,Jiewei Xu,Benjamin J Polacco,Ravi V Desai,Nilanjana Chatterjee,Austin Hsu,Jonathon M Muncie,Aaron M Blotnick,Sarah A B Winchester,Leor S Weinberger,Ruth Hüttenhain,Irfan S Kathiriya,Nevan J Krogan,Jeffrey J Saucerman,Benoit G Bruneau

eLife 9: PubMed32436841

2020

Axon-dependent expression of YAP/TAZ mediates Schwann cell remyelination but not proliferation after nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Grove,Hyunkyoung Lee,Huaqing Zhao,Young-Jin Son

Development (Cambridge, England) 146: PubMed31427287

2019

Teashirt 1 (Tshz1) is essential for the development, survival and function of hypoglossal and phrenic motor neurons in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Chaimowicz,Pierre-Louis Ruffault,Cyril Chéret,Andrew Woehler,Niccolò Zampieri,Gilles Fortin,Alistair N Garratt,Carmen Birchmeier

Science advances 4:eaar4471 PubMed29740613

2018

BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Miyamoto,Tomohiro Torii,Kenji Tago,Akito Tanoue,Shou Takashima,Junji Yamauchi

eLife 6: PubMed28124973

2017

YAP/TAZ initiate and maintain Schwann cell myelination.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Grove,Hyukmin Kim,Maryline Santerre,Alexander J Krupka,Seung Baek Han,Jinbin Zhai,Jennifer Y Cho,Raehee Park,Michele Harris,Seonhee Kim,Bassel E Sawaya,Shin H Kang,Mary F Barbe,Seo-Hee Cho,Michel A Lemay,Young-Jin Son

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Oct6 antibody [EP5421]

Western blot - Anti-Oct6 antibody [EP5421] (AB126746)

OI-RD Scanning - Anti-Oct6 antibody [EP5421] (AB126746)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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