AB197029

Anti-ODF1 antibody [EPR15496]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal ODF1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 6 publications.

View Alternative Names

Odfp, Odf1, Outer dense fiber protein 1

1 Images

Supplier Data

Western blot - Anti-ODF1 antibody [EPR15496] (AB197029)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ODF1 antibody [EPR15496] (ab197029) at 1/20000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Lane 4:

Mouse kidney lysate at 10 µg

Lane 5:

Mouse spleen lysate at 10 µg

Lane 6:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

Lane 7:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

true

Exposure time: 30s

Carrier free

Anti-ODF1 antibody [EPR15496] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15496

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ODF1 also known as Outer Dense Fiber Protein 1 plays an important role in the structural integrity of sperm flagella. The protein has a molecular mass of approximately 29 kDa. It localizes particularly to the outer dense fibers of sperm cells an important site for motility. Expression of ODF1 occurs primarily in the testis especially in mature spermatozoa where it contributes to the stability and function of the sperm tail.

Biological function summary

ODF1 contributes significantly to sperm motility and structural support within the sperm flagellum. As part of more complex structures ODF1 interacts with other potential flagellar components to ensure adequate mechanical support during sperm movement. By providing this stability ODF1 enables sperm cells to withstand the mechanical forces they encounter in the reproductive tract which is essential for male fertility.

Pathways

Several critical molecular interactions link ODF1 to reproductive success and sperm motility. Notably it plays a role within the sperm motility pathway and is interconnected with other structural proteins such as AKAP3 (A-Kinase Anchoring Protein 3) to provide anchoring points and scaffolding within the sperm tail. These pathways maintain proper structural function which is important for efficient motility and fertility.

Dysfunction or mutations in ODF1 may lead to male fertility issues such as asthenozoospermia characterized by reduced sperm motility. Research suggests a potential interaction with related proteins such as SPAG16 (Sperm Associated Antigen 16) which may further influence motility and structural integrity. Understanding these relationships can help in creating targeted therapies and diagnostics for reproductive health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the outer dense fibers (ODF) of spermatozoa. ODF are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail.

See full target information Odf1

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30953 PubMed40846889

2025

Esculetin inhibits ferroptosis after ICH by promoting NUDT1-mediated m7G methylation modification of GPX4.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbing Gong,Zhanwei Zhang,Libo Li,Zhigang Mei,Tao Chen

Cellular and molecular life sciences : CMLS 82:69 PubMed39899042

2025

Dnajc5b contributes to male fertility by maintaining the mitochondrial functions and autophagic homeostasis during spermiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dake Chen,Shiqin Zhou,Jinhua Tang,Hao Xiong,Jialian Li,Fenge Li

Heliyon 10:e38263 PubMed39386799

2024

WDR64, a testis-specific protein, is involved in the manchette and flagellum formation by interacting with ODF1.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfei Zhang,Xiaowei Xing,Lihua Huang,Yuyan Su,Gang Liu,Xinxing Zhang,Youbo Yang

Biomedicines 12: PubMed38275411

2024

The Role of Chorein Deficiency in Late Spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaoru Arai,Yoshiaki Nishizawa,Omi Nagata,Hitoshi Sakimoto,Natsuki Sasaki,Akira Sano,Masayuki Nakamura

Animal genetics 54:446-456 PubMed37211688

2023

SPAG6 c.900 T>C affects boar semen quality and blood-testis barrier function by creating a new splice acceptor site.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Bai,Dake Chen,Hao Xiong,Huibin Song,Tiansu Wang,Xinpeng Yang,Jinhua Tang,Yue Feng,Jialian Li,Fenge Li

Scientific reports 10:15659 PubMed32973212

2020

Novel targets identified by integrated proteomic and phosphoproteomic analysis in spermatogenesis of swamp buffalo (Bubalus bubalis).

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Lin Huang,Peng-Fei Zhang,Qiang Fu,Weng-Tan He,Kai Xiao,Ming Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ODF1 antibody [EPR15496]

Western blot - Anti-ODF1 antibody [EPR15496] (AB197029)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise