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AB251236

Anti-ODF1 antibody [EPR15496] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ODF1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Odfp, Odf1, Outer dense fiber protein 1

1 Images
Western blot - Anti-ODF1 antibody [EPR15496] - BSA and Azide free (AB251236)
  • WB

Supplier Data

Western blot - Anti-ODF1 antibody [EPR15496] - BSA and Azide free (AB251236)

This data was developed using ab197029, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ODF1 antibody [EPR15496] (<a href='/en-us/products/primary-antibodies/odf1-antibody-epr15496-ab197029'>ab197029</a>) at 1/20000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Lane 4:

Mouse kidney lysate at 10 µg

Lane 5:

Mouse spleen lysate at 10 µg

Lane 6:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

Lane 7:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15496

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251236 is the carrier-free version of ab197029.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ODF1 also known as Outer Dense Fiber Protein 1 plays an important role in the structural integrity of sperm flagella. The protein has a molecular mass of approximately 29 kDa. It localizes particularly to the outer dense fibers of sperm cells an important site for motility. Expression of ODF1 occurs primarily in the testis especially in mature spermatozoa where it contributes to the stability and function of the sperm tail.
Biological function summary

ODF1 contributes significantly to sperm motility and structural support within the sperm flagellum. As part of more complex structures ODF1 interacts with other potential flagellar components to ensure adequate mechanical support during sperm movement. By providing this stability ODF1 enables sperm cells to withstand the mechanical forces they encounter in the reproductive tract which is essential for male fertility.

Pathways

Several critical molecular interactions link ODF1 to reproductive success and sperm motility. Notably it plays a role within the sperm motility pathway and is interconnected with other structural proteins such as AKAP3 (A-Kinase Anchoring Protein 3) to provide anchoring points and scaffolding within the sperm tail. These pathways maintain proper structural function which is important for efficient motility and fertility.

Dysfunction or mutations in ODF1 may lead to male fertility issues such as asthenozoospermia characterized by reduced sperm motility. Research suggests a potential interaction with related proteins such as SPAG16 (Sperm Associated Antigen 16) which may further influence motility and structural integrity. Understanding these relationships can help in creating targeted therapies and diagnostics for reproductive health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the outer dense fibers (ODF) of spermatozoa. ODF are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail.
See full target information Odf1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:3361-3370 PubMed33682317

2021

TNFAIP8 regulates gastric cancer growth via mTOR-Akt-ULK1 pathway and autophagy signals.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Chen,Jianguo Zhang,Chenyang Dong,Dongsheng Li,Yuehan Yin,Wenhai Yu,Yuezhi Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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