AB222837

Anti-OFD1 antibody

Name: Anti-OFD1 antibody (ab222837) | Abcam
Brand: Abcam Limited
SKU: AB222837

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(8 Publications)

Rabbit Polyclonal OFD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OFD1 aa 550-800.

View Alternative Names

CXorf5, OFD1, Centriole and centriolar satellite protein OFD1, Oral-facial-digital syndrome 1 protein, Protein 71-7A

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OFD1 antibody (AB222837)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OFD1 antibody (AB222837)

Paraffin-embedded human adrenal gland tissue stained for OFD1 using ab222837 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OFD1 antibody (AB222837)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling OFD1 with ab222837 at 1/100 dilution, followed by a Goat anti-Mouse IgG antibody labelled by HRP and visualised using 0.05% DAB.

Antigen retrieval was performed using high pressure in a citrate buffer, pH 6.0. Section was blocked with 10% normal goat serum for 30 minutes at 37°C, before being incubated with the primary antibody at 4°C overnight.

Immunocytochemistry/ Immunofluorescence - Anti-OFD1 antibody (AB222837)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OFD1 antibody (AB222837)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for OFD1 (green) using ab222837 at 1/100 dilution in ICC/IF. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) was used as secondary antibody.

Supplier Data

Western blot - Anti-OFD1 antibody (AB222837)

Lane 1 : Hela whole cell lysate.

Lane 2 : SH-SY5Y whole cell lysate.

Lane 3 : K562 whole cell lysate.

All lanes : ab222837 antibody at 1 : 2000 dilution.

Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.

Predicted band size : 117, 43, 112 kDa Observed band size : 117 kDa.

All lanes:

Western blot - Anti-OFD1 antibody (ab222837)

Predicted band size: 116 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human OFD1 aa 550-800. The exact immunogen used to generate this antibody is proprietary information.

O75665

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

>95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The OFD1 protein also known as Oral-Facial-Digital Syndrome 1 protein has an important mechanical function in the formation of centrioles and basal bodies important for ciliary development and function. This protein weighs approximately 123 kDa and is found prominently in tissues with motile and non-motile cilia including the brain kidneys and lungs. OFD1 is expressed in both embryonic and adult tissues indicating its role across developmental stages.

Biological function summary

The OFD1 protein is a critical part of the centrosome and cilia structure. It is prominently involved in the centrosome-cilium interface ensuring proper structure and function of cilia which are essential for cell signaling and movement. OFD1 interacts with proteins like CEP290 and BBS4 within the basal body and centriolar satellites indicating its participation in maintaining ciliary architecture and function.

Pathways

The OFD1 protein is involved in pathways that influence cilia-related signaling. One significant pathway includes the Hedgehog signaling pathway which relies on cilia for signal transduction. Proteins such as Gli and Smoothened which play roles within these pathways interact with ciliary structures supported by OFD1 highlighting its involvement in signaling required for tissue development and morphogenesis.

Disruption in OFD1 function relates to oral-facial-digital syndrome type 1 and Joubert syndrome both of which are ciliopathies. These disorders arise due to defects in cilia leading to problems in multiple organ systems including the nervous system and kidneys. OFD1's interactions with CEP290 become critical in the context of these diseases as defects in these protein interactions can cause the ciliopathies' phenotypes explaining the mutations' contributions to clinical conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164 (By similarity). Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis (PubMed : 33934390). Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation (By similarity).

See full target information OFD1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38892227

2024

SMYD3 Controls Ciliogenesis by Regulating Distinct Centrosomal Proteins and Intraflagellar Transport Trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Ewud Agborbesong,Julie Xia Zhou,Hongbing Zhang,Linda Xiaoyan Li,Peter C Harris,James P Calvet,Xiaogang Li

Neuron 111:65-80.e6 PubMed36334595

2022

CROCCP2 acts as a human-specific modifier of cilia dynamics and mTOR signaling to promote expansion of cortical progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Roxane Van Heurck,Jérôme Bonnefont,Marta Wojno,Ikuo K Suzuki,Fausto D Velez-Bravo,Emir Erkol,Dan Truc Nguyen,Adèle Herpoel,Angéline Bilheu,Sofie Beckers,Catherine Ledent,Pierre Vanderhaeghen

Journal of cell science 135: PubMed35343570

2022

PLK1 controls centriole distal appendage formation and centrobin removal via independent pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Morgan Le Roux-Bourdieu,Devashish Dwivedi,Daniela Harry,Patrick Meraldi

International journal of biological sciences 16:2835-2852 PubMed33061800

2020

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Dong,Mei Feng,Hui Yang,Hengkang Liu,Hua Guo,Xianshu Gao,Yucun Liu,Rong Liu,Ning Zhang,Ruibing Chen,Ruirui Kong

Oncology reports 44:939-958 PubMed32705238

2020

Comparative proteomic analysis uncovers potential biomarkers involved in the anticancer effect of Scutellarein in human gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Venu Venkatarame Gowda Saralamma,Preethi Vetrivel,Ho Jeong Lee,Seong Min Kim,Sang Eun Ha,Rajeswari Murugesan,Eun Hee Kim,Jeong Doo Heo,Gon Sup Kim

PLoS biology 18:e3000620 PubMed32053600

2020

NRF2 negatively regulates primary ciliogenesis and hedgehog signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Liu,Matthew Dodson,Deyu Fang,Eli Chapman,Donna D Zhang

Cell reports 30:1447-1462.e5 PubMed32023461

2020

Wnt3a Stimulation Promotes Primary Ciliogenesis through β-Catenin Phosphorylation-Induced Reorganization of Centriolar Satellites.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Lang Kyun,Sun-Ok Kim,Hee Gu Lee,Jeong-Ah Hwang,Joonsung Hwang,Nak-Kyun Soung,Hyunjoo Cha-Molstad,Sangku Lee,Yong Tae Kwon,Bo Yeon Kim,Kyung Ho Lee

Journal of cell science 131: PubMed30463852

2018

Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Ju Hsiao,Chia-Hsiang Chang,Ridwan Babatunde Ibrahim,I-Hsuan Lin,Chun-Hung Wang,Won-Jing Wang,Jin-Wu Tsai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-OFD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OFD1 antibody (AB222837)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OFD1 antibody (AB222837)

Immunocytochemistry/ Immunofluorescence - Anti-OFD1 antibody (AB222837)

Western blot - Anti-OFD1 antibody (AB222837)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise