Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody

• IP

Supplier Data

Immunoprecipitation - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5μg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody.

All lanes:

Immunoprecipitation - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)

Predicted band size: 116 kDa

false

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling O-GlcNAc transferase protein at nucleus using ab96718 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red).
Scale bar = 10 µM

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1 : 500 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for O-GlcNAc transferase protein at cytoplasm and nucleus with ab96718 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for O-GlcNAc transferase protein at cytoplasm and nucleus with ab96718 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for O-GlcNAc transferase protein at cytoplasm and nucleus with ab96718 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for O-GlcNAc transferase protein at cytoplasm and nucleus with ab96718 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for O-GlcNAc transferase protein at cytoplasm and nucleus with ab96718 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for O-GlcNAc transferase protein at cytoplasm and nucleus with ab96718 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for O-GlcNAc transferase protein at cytoplasm and nucleus with ab96718 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/5000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 117 kDa

false

• WB

Unknown

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

All lanes:

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/mL

Lane 1:

Marker

Lane 2:

Zebrafish brain homogenate at 20 µg

Lane 3:

Zebrafish heart homogenate at 20 µg

Lane 4:

Zebrafish liver homogenate at 20 µg

Lane 5:

Zebrafish skeletal muscle homogenate at 20 µg

Lane 6:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution

Predicted band size: 116 kDa

Observed band size: 117 kDa

true

Exposure time: 5min

• WB

Supplier Data

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/5000 dilution

Lane 1:

Neuro2A whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 117 kDa

false

• WB

Supplier Data

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/5000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 117 kDa

false

• WB

Supplier Data

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (AB96718)

5% SDS-PAGE

All lanes:

Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution

All lanes:

PC-12 whole cell extract at 30 µg

Predicted band size: 116 kDa

false