Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) is a rabbit polyclonal antibody that is used to detect OGT / O-Linked N-Acetylglucosamine Transferase in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat, Zebrafish samples.
- Over 30 publications
- Trusted since 2010
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Publications
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- International journal of molecular sciences 23:2022O-GlcNAcylation of SIRT1 Protects against Cold Stress-Induced Skeletal Muscle Damage via Amelioration of Mitochondrial Homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Cao,Meng Zhang,Ye Li,Jingjing Lu,Wanhui Zhou,Xiaoshuang Li,Hao Shi,Bin Xu,Shize LiPubMed 36498847
- International journal of molecular sciences 23:2022Intestinal Epithelial STAT6 Activation Rescues the Defective Anti-Helminth Responses Caused by Deletion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiwen Xiong,Rong Huang,Zun Li,Chenyan Yang,Qingzhi Wang,Hai-Bin Ruan,Lin XuPubMed 36232438
- International journal of molecular sciences 23:2022Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeng-Fei Wang,Zhi-Qiang Feng,Ya-Wen Sun,Shan-Jiang Zhao,Hui-Ying Zou,Hai-Sheng Hao,Wei-Hua Du,Xue-Ming Zhao,Hua-Bin Zhu,Yun-Wei PangPubMed 35887161
- Biomedicines 10:2022Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye Won Lee,Mi Ju Kang,Young-Ju Kwon,Sama Abdi Nansa,Eui Hyun Jung,Sung Han Kim,Sang-Jin Lee,Kyung-Chae Jeong,Youngwook Kim,Heesun Cheong,Ho Kyung SeoPubMed 35625898
- Cell discovery 8:402022Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Yu,Qianping Zhang,Hanyu Liu,Jinming Liu,Song Yang,Xiaofan Luo,Wei Liu,Hao Zheng,Qiqi Liu,Yunxi Cui,Guo Chen,Yanjun Li,Xinglu Huang,Xiyun Yan,Jun Zhou,Quan ChenPubMed 35504898
- Frontiers in molecular neuroscience 14:7299752021Proteomic Profiling of Astrocytic O-GlcNAc Transferase-Related Proteins in the Medial Prefrontal Cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Fan,Qiu-Ling Zhong,Ran Mo,Cheng-Lin Lu,Jing Ren,Jia-Wen Mo,Fang Guo,You-Lu Wen,Xiong CaoPubMed 34803603
- Oncogene 40:6707-67192021O-GlcNAc modified-TIP60/KAT5 is required for PCK1 deficiency-induced HCC metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Liu,Dongmei Gou,Jin Xiang,Xuanming Pan,Qingzhu Gao,Peng Zhou,Yi Liu,Jie Hu,Kai Wang,Ni TangPubMed 34650217
- Cell death & disease 12:8892021Corosolic acid inhibits cancer progression by decreasing the level of CDK19-mediated O-GlcNAcylation in liver cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCongcong Zhang,Yongjie Niu,Zhixian Wang,Xin Xu,Yan Li,Lifang Ma,Jiayi Wang,Yongchun YuPubMed 34588426
- Journal of cellular and molecular medicine 25:9168-91822021P53 suppresses the progression of hepatocellular carcinoma via miR-15a by decreasing OGT expression and EZH2 stabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenyu You,Dandan Peng,Yixin Cao,Yuanzhe Zhu,Jianjun Yin,Guangxing Zhang,Xiaodong PengPubMed 34510715
- Cell death discovery 7:1122021Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuomeng Qian,Xiaoshan Yang,Shaohui Li,Hang Zhao,Yunge Gao,Shuhui Zhao,Xiaohui Lv,Xiyuan Zhang,Lingxia Li,Lianghao Zhai,Fuxing Zhou,Biliang ChenPubMed 34001861
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human OGT aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link O15294
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Tested | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Zebrafish | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc) (PubMed:12150998, PubMed:15361863, PubMed:19451179, PubMed:20018868, PubMed:21240259, PubMed:21285374, PubMed:23103939, PubMed:26237509, PubMed:26369908, PubMed:26678539, PubMed:27713473, PubMed:37541260, PubMed:37962578). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, AMPK, ATG4B, CAPRIN1, EZH2, FNIP1, GSDMD, KRT7, LMNA, LMNB1, LMNB2, RPTOR, HOXA1, PFKL, KMT2E/MLL5, MAPT/TAU, TET2, RBL2, RET, NOD2 and HCFC1 (PubMed:19451179, PubMed:20200153, PubMed:21285374, PubMed:22923583, PubMed:23353889, PubMed:24474760, PubMed:26237509, PubMed:26369908, PubMed:26678539, PubMed:27527864, PubMed:30699359, PubMed:34074792, PubMed:34667079, PubMed:37541260, PubMed:37962578). Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (PubMed:21285374). Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling (By similarity). Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) (PubMed:22121020, PubMed:23353889). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (PubMed:24474760). Stabilizes KMT2E/MLL5 by mediating its glycosylation, thereby preventing KMT2E/MLL5 ubiquitination (PubMed:26678539). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver (By similarity). Stabilizes clock proteins BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation (By similarity). Promotes the CLOCK-BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (PubMed:21285374, PubMed:28302723, PubMed:28584052). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1 (PubMed:20200153). Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1 (By similarity). Promotes autophagy by mediating O-glycosylation of ATG4B (PubMed:27527864). Acts as a regulator of mTORC1 signaling by mediating O-glycosylation of RPTOR and FNIP1: O-GlcNAcylation of RPTOR in response to glucose sufficiency promotes activation of the mTORC1 complex (PubMed:30699359, PubMed:37541260). Isoform 2. The mitochondrial isoform (mOGT) is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line. Isoform 4. Has N-acetylglucosaminyltransferase activity: glycosylates proteins, such as HNRNPU, NEUROD1, NUP62 and PDCD6IP (PubMed:31527085). Displays specific substrate selectivity compared to other isoforms (PubMed:31527085).
Alternative names
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT
Recommended products
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) is a rabbit polyclonal antibody that is used to detect OGT / O-Linked N-Acetylglucosamine Transferase in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat, Zebrafish samples.
- Over 30 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human OGT aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link O15294
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat, Zebrafish samples.
What is the molecular weight of OGT / O-Linked N-Acetylglucosamine Transferase?
Anti-OGT / O-Linked N-Acetylglucosamine Transferase (ab96718) specifically detects a band for OGT / O-Linked N-Acetylglucosamine Transferase (UniProt: O15294) at a molecular weight of 117kDa.
Recommended positive controls
WB: HeLa, PC-12 cells. Mouse brain tissue extracts.ICC/IF: MCF7, DU145, HeLa cells.IHC-P: Cal27 Xenograft. Rat hind brain.IP: A431 cells.
Trusted by the scientific community
Anti-OGT / O-Linked N-Acetylglucosamine Transferase (ab96718) was first used in a scientific publication in 2010 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-OGT / O-Linked N-Acetylglucosamine Transferase (ab96718) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OGT or O-Linked N-Acetylglucosamine Transferase also known as OGT protein mechanically catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto serine or threonine residues of nuclear and cytoplasmic proteins. OGT has a molecular weight of approximately 110 kDa. It is expressed in various tissues with higher levels in the pancreas brain and spleen. This enzyme plays an important role in cellular processes by modifying proteins with O-GlcNAc.
- Biological function summary
Through its transferase activity OGT regulates a wide array of cellular functions including signal transduction transcription and metabolism. It is a component of a complex involving several other proteins that facilitate its regulatory actions. OGT manages protein function and stability by influencing interactions between proteins and other cellular components.
- Pathways
OGT is critical in the insulin signaling pathway and the regulation of glucose metabolism. It interacts with multiple proteins such as IRS-1 and Akt to modulate their activity and stability. The modification by OGT impacts protein phosphorylation states affecting the downstream processing of cellular signals.
- Associated diseases and disorders
OGT has a significant link to diabetes and neurodegenerative diseases like Alzheimer's. Abnormal OGT activity relates to insulin resistance contributing to diabetes pathogenesis. In Alzheimer's disease altered O-GlcNAcylation affects proteins like tau which play a central role in disease progression. Understanding OGT's role in these conditions could aid in developing targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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10 product images
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
7.5% SDS PAGE
All lanes: Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 116 kDa
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
All lanes: Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/mL
Lane 1: Marker
Lane 2: Zebrafish brain homogenate at 20 µg
Lane 3: Zebrafish heart homogenate at 20 µg
Lane 4: Zebrafish liver homogenate at 20 µg
Lane 5: Zebrafish skeletal muscle homogenate at 20 µg
Lane 6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 117 kDa
Exposure time: 5min
Immunoprecipitation - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5μg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody.
All lanes: Immunoprecipitation - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
Predicted band size: 116 kDa
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
HeLa cells stained for O-GlcNAc (green) using ab96718 at 1/200 dilution in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using ab96718 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
5% SDS-PAGE
All lanes: Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution
All lanes: PC-12 whole cell extract at 30 µg
Predicted band size: 116 kDa
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
Paraffin embedded rat hind brain tissue stained for O-GlcNAc using ab96718 at 1/500 in immunohistochemical analysis.
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
7.5% SDS-PAGE
All lanes: Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/2000 dilution
All lanes: Mouse brain tissue extract at 50 µg
Predicted band size: 116 kDa
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