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AB126749

Anti-OIF antibody [EPR6963]

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(2 Publications)

Rabbit Recombinant Monoclonal OIF antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

OIF, SLRR3A, OGN, Mimecan, Osteoglycin, Osteoinductive factor

2 Images
Western blot - Anti-OIF antibody [EPR6963] (AB126749)
  • WB

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Western blot - Anti-OIF antibody [EPR6963] (AB126749)

All lanes:

Western blot - Anti-OIF antibody [EPR6963] (ab126749) at 1/1000 dilution

Lane 1:

Human artery lysate at 10 µg

Lane 2:

Human skin lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 34 kDa

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OI-RD Scanning - Anti-OIF antibody [EPR6963] (AB126749)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-OIF antibody [EPR6963] (AB126749)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6963

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OIF also known as Ovulation Inducing Factor has a mass of about 14 kDa. It plays an important role in inducing ovulation in certain mammalian species. OIF is expressed in seminal plasma where it serves as a signaling molecule influencing the reproductive cycle. Researchers recognize the impact of OIF on the ovarian follicle where it initiates a cascade of events that lead to ovulation. Its discovery is significant for reproductive biology as it highlights the influence of male factors on female reproductive physiology.
Biological function summary

Ovulation Inducing Factor acts as a signaling molecule that interacts with the female reproductive system to trigger ovulation. It functions as part of a larger biochemical complex that affects the hypothalamus and pituitary gland axis stimulating the secretion of hormones necessary for the release of the egg. The exact interactions and pathways remain an active area of research but the ability of OIF to prompt ovulation positions it as a unique factor among reproductive proteins.

Pathways

There is evidence that Ovulation Inducing Factor is involved in the neuroendocrine pathway for ovulation. OIF interacts with the hypothalamus-pituitary-gonadal axis to initiate ovulation. The presence of other proteins like GnRH and its release is closely tied to the activity of OIF in stimulating the necessary hormonal environment. Furthermore the interplay between OIF and luteinizing hormone (LH) is also explored showing how these elements integrate into the broader signaling cascade essential for ovulation.

Researchers have associated Ovulation Inducing Factor with fertility issues. Disruptions or alterations in the pathway involving OIF can lead to ovulatory disorders impacting fertility in both females and indirectly males. Studies explore its relationship with proteins like FSH and LH which are critical in ovulatory processes. Understanding the role of OIF may advance treatment options for certain infertility problems offering insights into novel therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
See full target information OGN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40552983

2025

Acidosis attenuates the hypoxic stabilization of HIF-1α by activating lysosomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Bobby White,Zhenyi Wang,Matthew Dean,Johanna Michl,Natalia Nieora,Sarah Flannery,Iolanda Vendrell,Roman Fischer,Alzbeta Hulikova,Pawel Swietach

Proteomics 15:2934-44 PubMed25914152

2015

Unravelling the proteome of degenerative human mitral valves.

Applications

WB

Species

Human

Hwee Tong Tan,Teck Kwang Lim,Arthur Mark Richards,Theodoros Kofidis,Kristine Leok-Kheng Teoh,Lieng H Ling,Maxey C M Chung
View all publications
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Product promise

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