AB229090

Anti-OLA1 antibody

Name: Anti-OLA1 antibody (ab229090) | Abcam
Brand: Abcam Limited
SKU: AB229090

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(3 Publications)

Rabbit Polyclonal OLA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OLA1.

View Alternative Names

GTPBP9, PRO2455, PTD004, OLA1, Obg-like ATPase 1, DNA damage-regulated overexpressed in cancer 45, GTP-binding protein 9, DOC45

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-OLA1 antibody (AB229090)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OLA1 antibody (AB229090)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for OLA1 (green) using ab229090 at 1/200 dilution in ICC/IF. Cells were fixed in methanol.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OLA1 antibody (AB229090)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OLA1 antibody (AB229090)

Paraffin-embedded human colon carcinoma tissue stained for OLA1 using ab229090 at 1/250 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-OLA1 antibody (AB229090)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-OLA1 antibody (ab229090) at 1/1000 dilution

All lanes:

Mouse testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-OLA1 antibody (AB229090)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-OLA1 antibody (ab229090) at 1/1000 dilution

All lanes:

Rat testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-OLA1 antibody (AB229090)

All lanes:

Western blot - Anti-OLA1 antibody (ab229090) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human OLA1. The exact immunogen used to generate this antibody is proprietary information.

Q9NTK5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OLA1 also known as Obg-like ATPase 1 is a GTPase enzyme with a molecular weight of approximately 47 kDa. It belongs to the Obg family of P-loop GTPases. You can find OLA1 primarily in the cytoplasm but it's also present in the mitochondria under certain conditions. Studies show high expression of OLA1 in a variety of tissues including the liver brain and heart indicating its widespread presence and importance in cellular processes.

Biological function summary

This protein regulates cellular processes such as protein synthesis stress response and mitochondrial function. OLA1 acts as a molecular switch influencing the dynamics of the cytoskeleton and protein synthesis machinery. It is an integral part of a multi-protein complex involved in the regulation of translation initiation factor eIF2B suggesting a role in controlling protein production during stress conditions.

Pathways

The actions of OLA1 fit into broader networks controlling cellular stress and protein homeostasis. It plays a significant role in the oxidative stress pathway and impacts the unfolded protein response. OLA1 interacts with other proteins such as HSP70 and eIF2 modulating how cells adapt to the changing environment and ensuring proper protein folding and function. This highlights its integrative role in cellular adaptation mechanisms.

OLA1 shows associations with cancer and neurodegenerative diseases. Its overexpression or mutation can contribute to tumorigenesis by affecting cell growth and apoptosis. Research also links OLA1 to Parkinson's disease where it might influence mitochondrial dysfunction interacting with proteins like Parkin and PINK1. Understanding OLA1's involvement in these disorders offers potential insights for therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.

See full target information OLA1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70095 PubMed39500626

2024

Lysine Acetyltransferase TIP60 Restricts Nerve Injury by Activating IKKβ/SNAP23 Axis-Mediated Autophagosome-Lysosome Fusion in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Jun Min,Qinghua Luo,Xunhu Gu,Min Li,Xu Liu

BMC molecular and cell biology 21:65 PubMed32928102

2020

Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhou Liu,Qing Yang,Kevin Chen Xiao,Thomas Dobleman,Shen Hu,Gary Guishan Xiao

Aging 12:3025-3041 PubMed32045367

2020

Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shanzhou Huang,Chuanzhao Zhang,Chengjun Sun,Yuchen Hou,Yixi Zhang,Nga Lei Tam,Zekang Wang,Jia Yu,Bowen Huang,Hongkai Zhuang,Zixuan Zhou,Zuyi Ma,Zhonghai Sun,Xiaoshun He,Qi Zhou,Baohua Hou,Linwei Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OLA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-OLA1 antibody (AB229090)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OLA1 antibody (AB229090)

Western blot - Anti-OLA1 antibody (AB229090)

Western blot - Anti-OLA1 antibody (AB229090)

Western blot - Anti-OLA1 antibody (AB229090)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise