AB251035

Anti-OLF1 antibody [EPR15972] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal OLF1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

OLF1, OR5I1, Olfactory receptor 5I1, Olfactory receptor OR11-159, Olfactory receptor-like protein OLF1

2 Images

Supplier Data

Western blot - Anti-OLF1 antibody [EPR15972] - BSA and Azide free (AB251035)

This data was developed using ab190792, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OLF1 antibody [EPR15972] (<a href='/en-us/products/primary-antibodies/olf1-antibody-epr15972-ab190792'>ab190792</a>) at 1/10000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal heart lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Supplier Data

Western blot - Anti-OLF1 antibody [EPR15972] - BSA and Azide free (AB251035)

This data was developed using ab190792, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OLF1 antibody [EPR15972] (<a href='/en-us/products/primary-antibodies/olf1-antibody-epr15972-ab190792'>ab190792</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat kidney lysate at 10 µg

Lane 8:

Rat spleen lysate at 10 µg

Lane 9:

C6 cell lysate at 10 µg

Lane 10:

Raw264.7 cell lysate at 10 µg

Lane 11:

PC12 cell lysate at 10 µg

Lane 12:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Unconjugated

Anti-OLF1 antibody [EPR15972]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15972

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251035 is the carrier-free version of ab190792.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OLF1 also known as OLF-1-like protein or Olfactory neuron specific factor 1 functions mainly as a transcription factor. It weighs approximately 34 kDa and is primarily expressed in neurons and tissues related to the olfactory system. OLF1 binds specific DNA sequences to regulate the transcription of genes involved in olfactory receptor expression. It plays a critical role in the development and function of the olfactory system by influencing gene networks specific to olfactory receptors.

Biological function summary

OLF1 impacts the expression of olfactory receptor genes by interacting with other transcription factors within the nucleus. It functions as part of larger transcriptional complexes to modulate olfactory receptor gene expression. Through these activities OLF1 contributes significantly to the regulation of sensory perception in mammals. Its precise role in olfactory development and sensory neuron identity highlights its importance in neural biology.

Pathways

OLF1 is integrated into the olfactory signaling pathway. It cooperates with proteins like O/E-1 and O/E-3 to regulate olfactory receptor gene clusters. This regulation is important for odor detection and signal transduction in the olfactory pathway. The olfactory signaling pathway involves multiple stages including ligand binding and signal transduction in which OLF1 contributes through its transcriptional regulatory functions.

Alterations in OLF1 activity have been linked to anosmia a condition characterized by the loss of smell. Researchers also explore its involvement in neurodegenerative disorders where olfactory deficits are early symptoms such as Parkinson’s disease. Examining OLF1 interactions with proteins such as alpha-synuclein provides insights into its potential role in the pathogenesis of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Odorant receptor.

See full target information OR5I1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OLF1 antibody [EPR15972] - BSA and Azide free

Western blot - Anti-OLF1 antibody [EPR15972] - BSA and Azide free (AB251035)

Western blot - Anti-OLF1 antibody [EPR15972] - BSA and Azide free (AB251035)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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