Mouse Monoclonal OLFM4 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OLFM4.
Images
![Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--immunocytochemistry-img129918.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)")
![Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--flow-cytometry-intracellular-img56349.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human OLFM4. Database link Q6UX06
Reactivity data
Select an application| ICC | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 - 1/100 | Notes - |
Target data
Function
May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
Alternative names
GW112, UNQ362/PRO698, OLFM4, Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1
Recommended products
Mouse Monoclonal OLFM4 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OLFM4.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human OLFM4. Database link Q6UX06
- Carrier free
- Yes
- Clone number
- 12
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The OLFM4 protein also known as Olfactomedin 4 or GW112 has a molecular weight of around 57 kDa. It is a glycoprotein expressed mainly in human and some non-human primate tissues including the prostate and gastrointestinal tract. OLFM4 is a secreted protein found in various cells including those of the bone marrow and esophagus. It plays a role in innate immunity and is a part of the olfactomedin family which is involved in cell adhesion and migration processes.
- Biological function summary
OLFM4 functions in inhibiting bacterial growth by interfering with bacterial adhesion and survival. It is not part of a larger protein complex but interacts with cell surface proteins to exert its effects. OLFM4 acts in the regulation of cell differentiation and growth playing a significant part in maintaining the stem cell milieu in the intestine.
- Pathways
OLFM4 participates in cellular defense mechanisms primarily against microbial pathogens by involving in the activation of immune response pathways. It is associated with pathways such as the NF-kB signaling and Janus kinase (JAK) pathways. Through these pathways OLFM4 might interact with cytokines and other inflammatory mediators or modulators.
- Associated diseases and disorders
OLFM4 relates to cancer particularly gastric and colorectal cancer where its expression levels serve as potential biomarkers for disease progression. Additionally it associates with inflammatory bowel diseases impacting the mucosal immune response. In cancer OLFM4's interaction with proteins like MMP isoforms (matrix metalloproteinases) can influence tumor metastasis and invasiveness.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--immunocytochemistry-img129918.jpg "Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)")
Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)
Immunofluorescence staining of OLFM4 in A431 (human epidermoid carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276046 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with an AlexaFluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--flow-cytometry-intracellular-img56349.jpg "Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)")
Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)
Intracellular flow cytometric analysis of OLFM4 expression in DU145 cells. The cells were stained with ab276046 at 1/25 dilution, then a FITC-conjugated second step antibody. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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