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AB276046

Anti-OLFM4 antibody [12] - BSA and Azide free

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Mouse Monoclonal OLFM4 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OLFM4.

View Alternative Names

GW112, UNQ362/PRO698, OLFM4, Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1

2 Images
Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)

Intracellular flow cytometric analysis of OLFM4 expression in DU145 cells. The cells were stained with ab276046 at 1/25 dilution, then a FITC-conjugated second step antibody. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)
  • ICC

Unknown

Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)

Immunofluorescence staining of OLFM4 in A431 (human epidermoid carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276046 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with an AlexaFluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

12

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human OLFM4.

Q6UX06

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Complementary Products

Assay Kits

AB267805

Human OLFM4 ELISA Kit

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ELISA - Human OLFM4 ELISA Kit (AB267805)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Assay Kits

AB267805

Human OLFM4 ELISA Kit

Recombinant|SimpleStep

ELISA - Human OLFM4 ELISA Kit (AB267805)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The OLFM4 protein also known as Olfactomedin 4 or GW112 has a molecular weight of around 57 kDa. It is a glycoprotein expressed mainly in human and some non-human primate tissues including the prostate and gastrointestinal tract. OLFM4 is a secreted protein found in various cells including those of the bone marrow and esophagus. It plays a role in innate immunity and is a part of the olfactomedin family which is involved in cell adhesion and migration processes.
Biological function summary

OLFM4 functions in inhibiting bacterial growth by interfering with bacterial adhesion and survival. It is not part of a larger protein complex but interacts with cell surface proteins to exert its effects. OLFM4 acts in the regulation of cell differentiation and growth playing a significant part in maintaining the stem cell milieu in the intestine.

Pathways

OLFM4 participates in cellular defense mechanisms primarily against microbial pathogens by involving in the activation of immune response pathways. It is associated with pathways such as the NF-kB signaling and Janus kinase (JAK) pathways. Through these pathways OLFM4 might interact with cytokines and other inflammatory mediators or modulators.

OLFM4 relates to cancer particularly gastric and colorectal cancer where its expression levels serve as potential biomarkers for disease progression. Additionally it associates with inflammatory bowel diseases impacting the mucosal immune response. In cancer OLFM4's interaction with proteins like MMP isoforms (matrix metalloproteinases) can influence tumor metastasis and invasiveness.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
See full target information OLFM4
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Product promise

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