Mouse Monoclonal OLFM4 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OLFM4.
Images
![Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--immunocytochemistry-img129918.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)")
![Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--flow-cytometry-intracellular-img56349.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (AB276046)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human OLFM4. Database link Q6UX06
Reactivity data
Select an application| ICC | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/100 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25 - 1/100 | Notes - |
Target data
Function
May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
Alternative names
Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1, OLFM4, GW112, UNQ362/PRO698
Recommended products
Mouse Monoclonal OLFM4 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human OLFM4.
Alternative names
Olfactomedin-4, OLM4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein, hOLfD, hGC-1, OLFM4, GW112, UNQ362/PRO698
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human OLFM4. Database link Q6UX06
- Carrier free
Yes
- Clone number
12
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The OLFM4 protein also known as Olfactomedin 4 or GW112 has a molecular weight of around 57 kDa. It is a glycoprotein expressed mainly in human and some non-human primate tissues including the prostate and gastrointestinal tract. OLFM4 is a secreted protein found in various cells including those of the bone marrow and esophagus. It plays a role in innate immunity and is a part of the olfactomedin family which is involved in cell adhesion and migration processes.
- Biological function summary
OLFM4 functions in inhibiting bacterial growth by interfering with bacterial adhesion and survival. It is not part of a larger protein complex but interacts with cell surface proteins to exert its effects. OLFM4 acts in the regulation of cell differentiation and growth playing a significant part in maintaining the stem cell milieu in the intestine.
- Pathways
OLFM4 participates in cellular defense mechanisms primarily against microbial pathogens by involving in the activation of immune response pathways. It is associated with pathways such as the NF-kB signaling and Janus kinase (JAK) pathways. Through these pathways OLFM4 might interact with cytokines and other inflammatory mediators or modulators.
- Associated diseases and disorders
OLFM4 relates to cancer particularly gastric and colorectal cancer where its expression levels serve as potential biomarkers for disease progression. Additionally it associates with inflammatory bowel diseases impacting the mucosal immune response. In cancer OLFM4's interaction with proteins like MMP isoforms (matrix metalloproteinases) can influence tumor metastasis and invasiveness.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--immunocytochemistry-img129918.jpg "Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)")
Immunocytochemistry - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)
Immunofluorescence staining of OLFM4 in A431 (human epidermoid carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276046 at 1/60 dilution at 37°C for 1 hour. Then cells were stained with an AlexaFluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
![Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046), expandable thumbnail](https://content.abcam.com/products/images/olfm4-antibody-12-bsa-and-azide-free-ab276046--flow-cytometry-intracellular-img56349.jpg "Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)")
Flow Cytometry (Intracellular) - Anti-OLFM4 antibody [12] - BSA and Azide free (ab276046)
Intracellular flow cytometric analysis of OLFM4 expression in DU145 cells. The cells were stained with ab276046 at 1/25 dilution, then a FITC-conjugated second step antibody. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com