Rabbit Recombinant Monoclonal OLIG1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Images
![Western blot - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (AB248054), expandable thumbnail](https://content.abcam.com/products/images/olig1-antibody-epr6790-bsa-and-azide-free-ab248054--western-blot-img115899.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (AB248054)")
![Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (AB248054), expandable thumbnail](https://content.abcam.com/products/images/olig1-antibody-epr6790-bsa-and-azide-free-ab248054--flow-cytometry-intracellular-img79986.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (AB248054)")
![OI-RD Scanning - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (AB248054), expandable thumbnail](https://content.abcam.com/products/images/olig1-antibody-epr6790-bsa-and-azide-free-ab248054--oi-rd-scanning-img95191.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (AB248054)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Expected |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube (By similarity).
Alternative names
BHLHB6, BHLHE21, OLIG1, Oligodendrocyte transcription factor 1, Oligo1, Class B basic helix-loop-helix protein 6, Class E basic helix-loop-helix protein 21, bHLHb6, bHLHe21
Recommended products
Rabbit Recombinant Monoclonal OLIG1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6790
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 2.4 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248054 is the carrier-free version of Anti-Olig1 antibody [EPR6790] ab124908.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Olig1 also known as oligodendrocyte lineage transcription factor 1 is a protein involved in the development and function of oligodendrocytes cells that form the myelin sheath in the central nervous system. It weighs approximately 30 kDa and is expressed primarily in the brain and spinal cord. The protein plays a fundamental role in the maturation process of oligodendrocyte progenitor cells by regulating the transcription of genes necessary for myelination.
- Biological function summary
Olig1 is important in the differentiation of progenitor cells into mature oligodendrocytes. It interacts with other proteins like Olig2 often forming heterodimer complexes that enhance transcriptional regulation. Olig1's regulatory actions influence cellular processes pivotal for maintaining neural integrity and function aiding in the production of myelin which is essential for fast and efficient nerve signal transmission in the central nervous system.
- Pathways
Olig1 contributes significantly to the oligodendrocyte differentiation and development pathway as well as the myelination process. It works closely with Olig2 with both proteins being regulated within the Sonic Hedgehog signaling pathway. This pathway is vital for neural cell fate determination and contributes to the development of the nervous system ensuring proper function and communication within central nervous system.
- Associated diseases and disorders
Olig1 plays a role in multiple sclerosis and leukodystrophies where myelination is impaired. Abnormal regulation or expression of Olig1 can lead to demyelination a characteristic feature of these diseases. It is also associated with Olig2 which is similarly implicated in these neurological disorders. Understanding Olig1's involvement opens potential targets for therapeutic approaches in treating diseases affecting myelin and oligodendrocyte function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054), expandable thumbnail](https://content.abcam.com/products/images/olig1-antibody-epr6790-bsa-and-azide-free-ab248054--western-blot-img115899.jpg "Western blot - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054)")
Western blot - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054)
This data was developed using Anti-Olig1 antibody [EPR6790] ab124908, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Olig1 antibody [EPR6790] (Anti-Olig1 antibody [EPR6790] ab124908) at 1/1000 dilution
Lane 1: MOLT4 cell lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 4: C6 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 35 kDa
![Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054), expandable thumbnail](https://content.abcam.com/products/images/olig1-antibody-epr6790-bsa-and-azide-free-ab248054--flow-cytometry-intracellular-img79986.jpg "Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054)")
Flow Cytometry (Intracellular) - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054)
This data was developed using Anti-Olig1 antibody [EPR6790] ab124908, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized 293T cells using Anti-Olig1 antibody [EPR6790] ab124908 at 1/10 dilution (red) or a rabbit IgG negative control (green).
![OI-RD Scanning - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054), expandable thumbnail](https://content.abcam.com/products/images/olig1-antibody-epr6790-bsa-and-azide-free-ab248054--oi-rd-scanning-img95191.jpg "OI-RD Scanning - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054)")
OI-RD Scanning - Anti-Olig1 antibody [EPR6790] - BSA and Azide free (ab248054)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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