AB254043

Anti-Olig2 antibody

Name: Anti-Olig2 antibody (ab254043) | Abcam
Brand: Abcam Limited
SKU: AB254043

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(3 Publications)

Rabbit Polyclonal Olig2 antibody. Suitable for WB, ICC/IF and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Olig2.

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody (AB254043)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody (AB254043)

Rat primary cortical neuroepithelial cells labeling Olig2 using ab254043 (red) at a 1/1000 dilution in ICC/IF. The cells were treated with basic FGF for 30 hrs prior to staining (this induces Olig2 expression in these primary cells).

Supplier Data

Western blot - Anti-Olig2 antibody (AB254043)

All lanes:

Western blot - Anti-Olig2 antibody (ab254043) at 1/3000 dilution

All lanes:

Neonatal rat brain lysate

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Olig2. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 8 Constituents: 2.42% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Olig2 also known as Oligo2 or OLIG2 is a protein with a molecular mass of around 32 kDa. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which play roles in neurodevelopment. Olig2 is expressed mainly in the central nervous system particularly in oligodendrocyte progenitor cells and motor neuron progenitors. As an important marker Olig2 staining is often used in studies involving these cell types to understand differentiation processes.

Biological function summary

Olig2 regulates gene expression important for the proper development of the central nervous system. It functions both independently and as a part of transcriptional complexes to influence the fate of neural progenitors. In particular its presence decides whether these progenitors will differentiate into neurons astrocytes or oligodendrocytes. Among the proteins it interacts with are factors that modulate neurogenesis and myelination highlighting its importance in maintaining normal brain homeostasis.

Pathways

Olig2 plays an important role in the oligodendrocyte differentiation and motor neuron generation pathways. It works closely with proteins such as SOX10 and NKX2.2 to regulate oligodendrocyte maturation. In the motor neuron pathway Olig2 interacts with proteins like NKX6.1 and LHX3 coordinating the development of motor neurons. These pathways demonstrate Olig2's function as a critical transcription factor influencing neural development and plasticity.

Abnormalities in Olig2 expression or function relate to conditions such as gliomas and Multiple Sclerosis (MS). Elevated levels of Olig2 are often seen in gliomas where it might cooperate with proteins like IDH1 to drive tumor growth. In MS the impairment of oligodendrocyte function can link back to disruptions in normal Olig2 activity or expression. These associations make Olig2 a significant target for potential therapeutic interventions in neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Olig2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102282 PubMed39176174

2024

Suppression of HSV-1 infection and viral reactivation by CRISPR-Cas9 gene editing in 2D and 3D culture models.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Bellizzi,Senem Çakır,Martina Donadoni,Rahsan Sariyer,Shuren Liao,Hong Liu,Guo-Xiang Ruan,Jennifer Gordon,Kamel Khalili,Ilker K Sariyer

Journal of neurovirology 30:362-379 PubMed38600307

2024

Modeling HIV-1 infection and NeuroHIV in hiPSCs-derived cerebral organoid cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Donadoni,Senem Cakir,Anna Bellizzi,Michael Swingler,Ilker K Sariyer

International journal of molecular sciences 22: PubMed33498219

2021

MST3 Involvement in Na and K Homeostasis with Increasing Dietary Potassium Intake.

Applications

Unspecified application

Species

Unspecified reactive species

Chee-Hong Chan,Sheng-Nan Wu,Bo-Ying Bao,Houng-Wei Li,Te-Ling Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Olig2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody (AB254043)

Western blot - Anti-Olig2 antibody (AB254043)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise