Mouse Monoclonal Olig2 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human OLIG2 aa 1-150.
Images
![Protein Array - Anti-Olig2 antibody [OLIG2/2400] - BSA and Azide free (AB237816), expandable thumbnail](https://content.abcam.com/products/images/olig2-antibody-olig2-2400-bsa-and-azide-free-ab237816--protein-array-img144328.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Olig2 antibody [OLIG2/2400] - BSA and Azide free (AB237816)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human OLIG2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13516
Reactivity data
Select an application| Protein Array | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Recombinant full length protein - Human | Tested | Not recommended |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 minutes at RT. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Alternative names
BHLHB1, BHLHE19, PRKCBP2, RACK17, OLIG2, Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19
Recommended products
Mouse Monoclonal Olig2 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human OLIG2 aa 1-150.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human OLIG2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q13516
- Carrier free
- Yes
- Clone number
- OLIG2/2400
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Olig2 also known as Oligo2 or OLIG2 is a protein with a molecular mass of around 32 kDa. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which play roles in neurodevelopment. Olig2 is expressed mainly in the central nervous system particularly in oligodendrocyte progenitor cells and motor neuron progenitors. As an important marker Olig2 staining is often used in studies involving these cell types to understand differentiation processes.
- Biological function summary
Olig2 regulates gene expression important for the proper development of the central nervous system. It functions both independently and as a part of transcriptional complexes to influence the fate of neural progenitors. In particular its presence decides whether these progenitors will differentiate into neurons astrocytes or oligodendrocytes. Among the proteins it interacts with are factors that modulate neurogenesis and myelination highlighting its importance in maintaining normal brain homeostasis.
- Pathways
Olig2 plays an important role in the oligodendrocyte differentiation and motor neuron generation pathways. It works closely with proteins such as SOX10 and NKX2.2 to regulate oligodendrocyte maturation. In the motor neuron pathway Olig2 interacts with proteins like NKX6.1 and LHX3 coordinating the development of motor neurons. These pathways demonstrate Olig2's function as a critical transcription factor influencing neural development and plasticity.
- Associated diseases and disorders
Abnormalities in Olig2 expression or function relate to conditions such as gliomas and Multiple Sclerosis (MS). Elevated levels of Olig2 are often seen in gliomas where it might cooperate with proteins like IDH1 to drive tumor growth. In MS the impairment of oligodendrocyte function can link back to disruptions in normal Olig2 activity or expression. These associations make Olig2 a significant target for potential therapeutic interventions in neurological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Protein Array - Anti-Olig2 antibody [OLIG2/2400] - BSA and Azide free (ab237816), expandable thumbnail](https://content.abcam.com/products/images/olig2-antibody-olig2-2400-bsa-and-azide-free-ab237816--protein-array-img144328.jpg "Protein Array - Anti-Olig2 antibody [OLIG2/2400] - BSA and Azide free (ab237816)")
Protein Array - Anti-Olig2 antibody [OLIG2/2400] - BSA and Azide free (ab237816)
This data was produced with ab236540, the same antibody in a different formulation with BSA and Azide.
ab236540 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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