AB136253

Anti-Olig2 antibody - Oligodendrocyte Marker

Name: Anti-Olig2 antibody (ab136253) | Abcam
Brand: Abcam Limited
SKU: AB136253
Price: 510 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(26 Publications)

Anti-Olig2 antibody (ab136253) is a rabbit polyclonal antibody detecting Olig2 in IHC-P. Suitable for Human.

- Over 10 publications

View Alternative Names

BHLHB1, BHLHE19, PRKCBP2, RACK17, OLIG2, Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17, bHLHb1, bHLHe19

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody - Oligodendrocyte Marker (AB136253)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody - Oligodendrocyte Marker (AB136253)

Immunohistochemical analysis of human glioblastoma labelling Olig2 with ab136253 at 1/500 dilution. Tissue was pretreated with TE Buffer, pH 9.0.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Olig2.

Q9EQW6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Olig2 antibody (ab136253) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-Olig2 (ab136253) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

Constituents: 2.42% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Olig2 also known as Oligo2 or OLIG2 is a protein with a molecular mass of around 32 kDa. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which play roles in neurodevelopment. Olig2 is expressed mainly in the central nervous system particularly in oligodendrocyte progenitor cells and motor neuron progenitors. As an important marker Olig2 staining is often used in studies involving these cell types to understand differentiation processes.

Biological function summary

Olig2 regulates gene expression important for the proper development of the central nervous system. It functions both independently and as a part of transcriptional complexes to influence the fate of neural progenitors. In particular its presence decides whether these progenitors will differentiate into neurons astrocytes or oligodendrocytes. Among the proteins it interacts with are factors that modulate neurogenesis and myelination highlighting its importance in maintaining normal brain homeostasis.

Pathways

Olig2 plays an important role in the oligodendrocyte differentiation and motor neuron generation pathways. It works closely with proteins such as SOX10 and NKX2.2 to regulate oligodendrocyte maturation. In the motor neuron pathway Olig2 interacts with proteins like NKX6.1 and LHX3 coordinating the development of motor neurons. These pathways demonstrate Olig2's function as a critical transcription factor influencing neural development and plasticity.

Abnormalities in Olig2 expression or function relate to conditions such as gliomas and Multiple Sclerosis (MS). Elevated levels of Olig2 are often seen in gliomas where it might cooperate with proteins like IDH1 to drive tumor growth. In MS the impairment of oligodendrocyte function can link back to disruptions in normal Olig2 activity or expression. These associations make Olig2 a significant target for potential therapeutic interventions in neurological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

See full target information OLIG2

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 16:3218-3246 PubMed39304759

2024

Neural precursor cells rescue symptoms of Rett syndrome by activation of the Interferon γ pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Angelisa Frasca,Federica Miramondi,Erica Butti,Marzia Indrigo,Maria Balbontin Arenas,Francesca M Postogna,Arianna Piffer,Francesco Bedogni,Lara Pizzamiglio,Clara Cambria,Ugo Borello,Flavia Antonucci,Gianvito Martino,Nicoletta Landsberger

Frontiers in immunology 14:1271118 PubMed37942317

2023

Acriflavine, a HIF-1 inhibitor, preserves vision in an experimental autoimmune encephalomyelitis model of optic neuritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey J Anders,Benjamin W Elwood,Randy H Kardon,Oliver W Gramlich

iScience 26:107913 PubMed37810220

2023

mNSCs overexpressing transplantation facilitates cognitive recovery in a mouse model of traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Tongjie Ji,Ying Pang,Meng Cheng,Rui Wang,Xu Chen,Chunyu Zhang,Min Liu,Jing Zhang,Chunlong Zhong

Neurobiology of disease 185:106264 PubMed37597815

2023

Reactive astrocytes associated with prion disease impair the blood brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Rajesh Kushwaha,Yue Li,Natallia Makarava,Narayan P Pandit,Kara Molesworth,Konstantin G Birukov,Ilia V Baskakov

eNeuro 10: PubMed37491366

2023

Single-Nucleus Profiling Identifies Accelerated Oligodendrocyte Precursor Cell Senescence in a Mouse Model of Down Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Rusu,Bharti Kukreja,Taiyi Wu,Sophie J Dan,Min Yi Feng,Brian T Kalish

Fluids and barriers of the CNS 20:42 PubMed37296418

2023

Early postnatal microglial ablation in the Ccdc39 mouse model reveals adverse effects on brain development and in neonatal hydrocephalus.

Applications

Unspecified application

Species

Unspecified reactive species

Farrah N Brown,Eri Iwasawa,Crystal Shula,Elizabeth M Fugate,Diana M Lindquist,Francesco T Mangano,June Goto

International journal of molecular sciences 23: PubMed36430692

2022

Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia Weier,Michael Enders,Philipp Kirchner,Arif Ekici,Marc Bigaud,Christopher Kapitza,Jürgen Wörl,Stefanie Kuerten

Biomolecules 12: PubMed36291646

2022

Targeting Cholesterol Homeostasis Improves Recovery in Experimental Optic Neuritis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheyanne R Godwin,Jeffrey J Anders,Lin Cheng,Benjamin W Elwood,Randy H Kardon,Oliver W Gramlich

Frontiers in pharmacology 13:971243 PubMed36120298

2022

Effects of Tibetan medicine metacinnabar (β-HgS) combined with imipramine or sertraline on depression-like symptoms in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Qiao,Cen Li,Ming Zhang,Xingfang Zhang,Lixin Wei,Keshen Cao,Xiaoyuan Zhang,Hongtao Bi,Tingting Gao

Translational neuroscience 13:104-115 PubMed35734308

2022

Remimazolam induced cognitive dysfunction in mice via glutamate excitotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Hua Zhou,Cheng-Cheng Zhang,Ling Wang,Shan-Liang Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Olig2 antibody - Oligodendrocyte Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody - Oligodendrocyte Marker (AB136253)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

Product promise