AB230626

Anti-Olig3 antibody

Name: Anti-Olig3 antibody (ab230626) | Abcam
Brand: Abcam Limited
SKU: AB230626
Price: 605 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal OLIG3 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OLIG3 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BHLHB7, BHLHE20, OLIG3, Oligodendrocyte transcription factor 3, Oligo3, Class B basic helix-loop-helix protein 7, Class E basic helix-loop-helix protein 20, bHLHb7, bHLHe20

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Formalin-fixed, paraffin-embedded human skin tissue stained for Olig3 using ab230626 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Formalin-fixed, paraffin-embedded human testis tissue stained for Olig3 using ab230626 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Olig3 antibody (AB230626)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Olig3 antibody (AB230626)

Intracellular flow cytometric analysis ofU-251 MG (human brain glioma cell line) cell line labeling Olig3 with ab230626 at 1/10 dilution (green) compared with a negative control cell (blue).

FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Supplier Data

Western blot - Anti-Olig3 antibody (AB230626)

All lanes:

Western blot - Anti-Olig3 antibody (ab230626) at 1/1000 dilution

All lanes:

Mouse spleen lysate at 35 µg

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human OLIG3 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q7RTU3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Olig3 also known as Oligodendrocyte transcription factor 3 acts as a basic helix-loop-helix (bHLH) transcription factor. It has a mass of about 31 kDa and plays an important role in regulating gene expression. You typically find it expressed in developing neural tissues especially in the central nervous system. In humans it is located on chromosome 6 where its precise control is necessary for proper neural development.

Biological function summary

Olig3 regulates the development of specific neurons in the central nervous system. It does not operate alone but often forms heterodimers with other bHLH transcription factors to exert its influence. Researchers have shown its involvement in neurogenesis particularly in defining neuronal cell fate. By activating or repressing specific genes it fine-tunes the development of neural progenitor cells into specialized neuron types.

Pathways

Olig3 integrates into the Notch and Sonic Hedgehog signaling pathways. It influences neuronal differentiation through these pathways often interacting with proteins like Hes1 which is part of the Notch pathway. Sonic Hedgehog also plays a role in guiding the spatial expression patterns where Olig3 is active ensuring a balanced developmental process. These pathways are essential for the correct propagation of signals that dictate the specification of neuronal cell lineages.

Olig3 has been implicated in medulloblastoma and certain developmental disorders. Dysregulation of Olig3 expression and function can contribute to tumor formation in the cerebellum often in conjunction with associated proteins such as Gli1 from the Sonic Hedgehog pathway. Abnormalities in Olig3 expression may also link to developmental neuroissues due to its foundational role in neuron formation and differentiation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.

See full target information OLIG3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 83:3783-3795 PubMed37668527

2023

A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody-Drug Conjugate for Targeting Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shi,Tao Jiao,Qian Guo,Weining Weng,Linjie Ma,Qing Zhang,Lijun Wang,Jianjian Zhang,Caiwei Chen,Yaling Huang,Mingqiao Wang,Rong Pan,Yanfang Tang,Wenhao Hu,Tao Meng,Shu-Hui Liu,Jun Guo,Yan Kong,Xun Meng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Olig3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig3 antibody (AB230626)

Flow Cytometry (Intracellular) - Anti-Olig3 antibody (AB230626)

Western blot - Anti-Olig3 antibody (AB230626)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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