AB249885

Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Oligodendrocyte Specific Protein antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

OSP, OTM, CLDN11, Claudin-11, Oligodendrocyte-specific protein

2 Images

Flow Cytometry - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - BSA and Azide free (AB249885)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - BSA and Azide free (AB249885)

This data was developed using ab175236, the same antibody clone in a different buffer formulation.

Flow Cytometrical analysis of U87-MG cells labeling Oligodendrocyte Specific Protein with ab175236 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - BSA and Azide free (AB249885)

This data was developed using ab175236, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - Oligodendrocyte Marker (<a href='/en-us/products/primary-antibodies/oligodendrocyte-specific-protein-antibody-epr12726-oligodendrocyte-marker-ab175236'>ab175236</a>) at 1/1000 dilution

Lane 1:

Human oligodendroglioma lysate at 10 µg

Lane 2:

U87-MG lysate at 10 µg

Lane 3:

SH-SY5Y lysate at 10 µg

Lane 4:

Fetal brain lysate at 10 µg

Lane 5:

Human testis lysate at 10 µg

Predicted band size: 22 kDa

false

Unconjugated

Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - Oligodendrocyte Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12726

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249885 is the carrier-free version of ab175236.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oligodendrocyte Specific Protein also known as O4 or O4 protein is an important marker for oligodendrocytes. This protein has a mass of approximately 24 kDa. Predominantly it is expressed in the central nervous system (CNS) specifically in oligodendrocyte precursor cells (OPCs) and mature oligodendrocytes. Its presence marks the differentiation stage of oligodendrocytes serving as an indicator of their development and maturation.

Biological function summary

The oligodendrocyte marker O4 plays a significant role in the myelination process supporting the formation of the myelin sheath around neurons. O4 does not act as part of a complex but is essential in the identification of oligodendrocyte lineage cells. During development it signals the transition of OPCs into mature myelinating oligodendrocytes which are necessary for effective neuronal signal transmission in the CNS.

Pathways

Oligodendrocyte marker O4 influences critical processes in the nervous system namely the myelination pathway. It interacts with various proteins including myelin basic protein (MBP) involved in the formation and maintenance of the myelin sheath. The presence of O4 marks the progression along the oligodendrocyte lineage and fits within the larger framework of CNS development and repair indicating a healthy state of neuronal insulation.

Disruptions involving the oligodendrocyte marker O4 can relate to multiple sclerosis (MS) and leukodystrophies. These conditions arise from demyelination or abnormal myelination respectively. O4 in association with other proteins like MBP and proteolipid protein (PLP) can serve as a biomarker for disease activity and progression. Its expression levels may assist in the research and diagnosis of demyelinating diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

See full target information CLDN11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - BSA and Azide free

Flow Cytometry - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - BSA and Azide free (AB249885)

Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR12726] - BSA and Azide free (AB249885)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise