Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human cerebrum.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining Ly-6K with ab246486 at a 1/4000 dilution, ab267377 anti-Wilms tumor protein used at an appropriate dilution and ab323317 anti-Oligodendrocyte Specific Protein used at an appropriate dilution.

Panel A : merged staining of anti-Ly-6K (green; Opal^™520), anti-Wilms tumor protein (magenta; Opal^™690) and anti-Oligodendrocyte Specific Protein (gray; Opal^™570) on human testis.

Panel B : anti-Ly-6K staining spermatocytes in human testis.

Panel C : anti-Wilms tumor protein staining Sertoli cells in human testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab246486, ab267377 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit using Opal Polymer HRP Ms + Rb secondary reagent (Ready to use). Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining GPC2 with ab324378 at a 1/200 dilution, ab323317 anti-Oligodendrocyte Specific Protein used at 1/500 dilution and ab323802 anti-MAGEA4 used at a 1/1000 dilution.

Panel A : anti-GPC2 (green; Opal™520), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-MAGEA4 (gray; Opal™570) on human testis.

Panel B : anti-GPC2 staining early spermatids in human testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Panel D : anti-MAGEA4 staining spermatogonia in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324378, ab323317 and ab323802 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining ATAD2 with ab324560 at a 1/100 dilution, ab323317 anti-Oligodendrocyte Specific Protein used at 1/500 dilution and ab315800 anti-CYP51A1/CYP51 used at a 1/2000 dilution.

Panel A : anti-ATAD2 (green; Opal™570), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-CYP51A1/CYP51 (gray; Opal™520) on human testis.

Panel B : anti-ATAD2 staining nucleus of proliferating cells in human testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Panel D : anti-CYP51A1/CYP51 staining Leydig cells in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324560, ab323317 and ab315800 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human spleen.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag cell pellet. (B) HEK-293T transfected with a CLDN11 expression vector containing a myc-His-tag cell pellet. tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

No staining in (A) HEK-293T transfected with an empty vector containing a His-tag cell pellet, positive staining in (B) HEK-293T transfected with a CLDN11 expression vector containing a myc-His-tag cell pellet.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining CT45 with ab323553 at a 1/1000 dilution, ab315275 anti-LAMA1 used at 1/500 dilution and ab323317 anti-Oligodendrocyte Specific Protein used at a 1/500 dilution.

Panel A : anti-CT45 (green; Opal™520), anti-LAMA1 (magenta; Opal™690) and anti-Oligodendrocyte Specific Protein (gray; Opal™570) on human testis.

Panel B : anti-CT45 staining nucleus in human testis.

Panel C : anti-LAMA1 staining basement membrane in human testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323553, ab315275 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-LAMA1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis. Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis. Panel C : anti-LAMA1 staining basement membrane in human testis. Panel D : anti-DDX4/MVH staining spermatogonia in human testis. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab323317, ab315275 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315275 anti-LAMA1 used at 1/500 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-LAMA1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Panel C : anti-LAMA1 staining basement membrane in human testis.

Panel D : anti-DDX4/MVH staining spermatogonia in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315275 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315800 anti-CYP51A1 used at 1/2000 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-CYP51A1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Panel C : anti-CYP51A1 staining Leydig cells in human testis.

Panel D : anti-DDX4/MVH staining spermatogonia in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315800 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human testis.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining SAGE1 with ab233388 at a 1/250 dilution, ab267377 anti-Wilms tumor protein used at an appropriate dilution and ab323317 anti-Oligodendrocyte Specific Protein used at an appropriate dilution.

Panel A : merged staining of anti-SAGE1 (green; Opal^™520), anti-Wilms tumor protein (magenta; Opal^™690) and anti-Oligodendrocyte Specific Protein (gray; Opal^™570) on human testis.

Panel B : anti-SAGE1 staining spermatogonia in human testis.

Panel C : anti-Wilms tumor protein staining Sertoli cells in human testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab233388, ab267377 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit using Opal Polymer HRP Ms + Rb Ready to use secondary reagent. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in rat cerebrum.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in rat spleen.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in rat testis.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse testis.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining Laminin alpha 1 with ab307542 at a 1/100 dilution, ab267377 anti-Wilms tumor protein used at 1/2000 dilution and ab323317 anti-Oligodendrocyte Specific Protein used at a 1/500 dilution.

Panel A : anti-Laminin alpha 1 (green; Opal™520), anti-Wilms tumor protein (magenta; Opal™690) and anti Oligodendrocyte Specific Protein (gray; Opal™570) on mouse testis.

Panel B : anti-Laminin alpha 1 staining basement membrane in mouse testis.

Panel C : anti-Wilms tumor protein staining Sertoli cells in mouse testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in mouse testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307542, ab267377 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse cerebrum.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315800 anti-CYP51A1 used at 1/2000 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-CYP51A1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on rat testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in rat testis.

Panel C : anti-CYP51A1 staining Leydig cells in rat testis.

Panel D : anti-DDX4/MVH staining spermatogonia in rat testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315800 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining Oligodendrocyte Specific Protein with ab323317 at a 1/500 dilution, ab315800 anti-CYP51A1 used at 1/2000 dilution and ab270534 anti-DDX4/MVH used at a 1/2000 dilution.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-CYP51A1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on mouse testis.

Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in mouse testis.

Panel C : anti-CYP51A1 staining Leydig cells in mouse testis.

Panel D : anti-DDX4/MVH staining spermatogonia in mouse testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab323317, ab315800 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining DMC1 with ab245217 at a 1/4000 dilution (0.168 μg/ml), ab267377 anti-Wilms tumor protein used at an appropriate dilution and ab323317 anti-Oligodendrocyte Specific Protein used at an appropriate dilution.

Panel A : merged staining of anti-DMC1 (green; Opal^™520), anti-Wilms tumor protein (magenta; Opal^™690) and anti-Oligodendrocyte Specific Protein (gray; Opal^™570) on mouse testis.

Panel B : anti-DMC1 staining early zygotene spermatocytes in mouse testis.

Panel C : anti-Wilms tumor protein staining Sertoli cells in mouse testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in mouse testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab245217, ab267377 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit using a ready to use Opal Polymer HRP Ms + Rb secondary reagent. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining DMC1 with ab245217 at a 1/4000 dilution (0.168 μg/ml), ab267377 anti-Wilms tumor protein used at an appropriate dilution and ab323317 anti-Oligodendrocyte Specific Protein used at an appropriate dilution.

Panel A : merged staining of anti-DMC1 (green; Opal^™520), anti-Wilms tumor protein (magenta; Opal^™690) and anti-Oligodendrocyte Specific Protein (gray; Opal^™570) on rat testis.

Panel B : anti-DMC1 staining early zygotene spermatocytes in rat testis.

Panel C : anti-Wilms tumor protein staining Sertoli cells in rat testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in rat testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab245217, ab267377 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit using a ready to use Opal Polymer HRP Ms + Rb secondary reagent. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining Laminin alpha 1 with ab307542 at a 1/100 dilution, ab267377 anti-Wilms tumor protein used at 1/2000 dilution and ab323317 anti-Oligodendrocyte Specific Protein used at a 1/500 dilution.

Panel A : anti-Laminin alpha 1 (green; Opal™520), anti-Wilms tumor protein (magenta; Opal™690) and anti Oligodendrocyte Specific Protein (gray; Opal™570) on rat testis.

Panel B : anti-Laminin alpha 1 staining basement membrane in rat testis.

Panel C : anti-Wilms tumor protein staining Sertoli cells in rat testis.

Panel D : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in rat testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307542, ab267377 and ab323317 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining SCP1 with ab324729 at a 1/1000 ( 0.49 μg/ml) dilution, ab323317 anti-Oligodendrocyte Specific Protein used at a 1/500 (1.032 μg/ml) dilution and ab267377 anti-Wilms tumor protein used at a 1/500 ( 0.706 μg/ml) dilution.

Panel A : merged staining of anti-SCP1 (green; Opal™520), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-Wilms tumor protein (gray; Opal™570) on mouse testis.

Panel B : anti-SCP1 staining spermatocytes in mouse testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in mouse testis.

Panel D : anti-Wilms tumor protein staining Sertoli cells in mouse testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324729, ab323317 and ab267377 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining SCP1 with ab324729 at a 1/1000 ( 0.49 μg/ml) dilution, ab323317 anti-Oligodendrocyte Specific Protein used at a 1/500 (1.032 μg/ml) dilution and ab267377 anti-Wilms tumor protein used at a 1/500 ( 0.706 μg/ml) dilution.

Panel A : merged staining of anti-SCP1 (green; Opal™520), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-Wilms tumor protein (gray; Opal™570) on rat testis.

Panel B : anti-SCP1 staining spermatocytes in rat testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in rat testis.

Panel D : anti-Wilms tumor protein staining Sertoli cells in rat testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324729, ab323317 and ab267377 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining ATAD2 with ab324560 at a 1/500 dilution, ab323317 anti-Oligodendrocyte Specific Protein used at 1/500 dilution and ab315800 anti-CYP51A1/CYP51 used at a 1/2000 dilution.

Panel A : anti-ATAD2 (green; Opal™570), anti-Oligodendrocyte Specific Protein (magenta; Opal™690) and anti-CYP51A1/CYP51 (gray; Opal™520) on mouse testis.

Panel B : anti-ATAD2 staining nucleus of proliferating cells in mouse testis.

Panel C : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in mouse testis.

Panel D : anti-CYP51A1/CYP51 staining Leydig cells in mouse testis.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324560, ab323317 and ab315800 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Oligodendrocyte Specific Protein with ab323317 at 1/500 (1.032 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse spleen.
The section was incubated with ab323317 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen(PMID : 10225958)

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (ab323317) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 21 kDa,124 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (AB323317)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen(PMID : 10225958)

The samples are unboiled.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Oligodendrocyte Specific Protein antibody [EPR29593-15] (ab323317) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 21 kDa,36 kDa

false

Exposure time: 15s