Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker

• WB

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Western blot - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (AB53041)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab53041) at 1/500 dilution

Lane 1:

Mouse brain extract

Lane 2:

Mouse brain extract with peptide

Predicted band size: 22 kDa

Observed band size: 22 kDa

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• WB

CiteAb

Western blot - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (AB53041)

Oligodendrocyte Specific Protein western blot using anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker ab53041. Publication image and figure legend from Hu, X., Tang, Z., et al., 2015, Sci Rep, PubMed 26265072.

ab53041 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab53041 please see the product overview.

BTB integrity was disrupted by Shp2 deletion.(A) BTB permeability was assayed with the biotin tracer (red) using confocal microscopy in two-week-old mice. Asterisk indicates the biotin tracer in the adluminal compartment of the seminiferous tubules. (B) Immunohistochemical staining of the BTB junction proteins Cx43, Claudin11 and JAMA in seminiferous tubules in two-week-old mice. (C) Altered BTB-related genes in the microarray gene database, and the protein levels of various genes were analyzed by Western blot in testes from two-week-old mice. The full-length blots are presented in Supplemental Figure S7. (D) The quantity of the cell junctions between primary Sertoli cells isolated from two-week-old mice was measured by transepithelial resistance (TER) assays. The experiments were repeated at least three times, and one representative result was shown. (E) Shp2 expression and Erk and Akt phosphorylation in primary Sertoli cells used in the TER assays. Tubulin was used as a loading control. The full-length blots are presented in Supplemental Figure S8. Ad, adenovirus virus; f/f, Shp2f/f cells; ko, SCSKO cells; Q79P, constitutively active Shp2 mutant. The values are expressed as the mean ± SEM. Statistical analysis was performed using Student's t-test. Asterisks denote the statistical significance, **p < 0.01; ***p < 0.001.

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