Rabbit Monoclonal Oncostatin M/OSM antibody. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (AB323383), expandable thumbnail](https://content.abcam.com/products/images/oncostatin-m-osm-antibody-epr26066-16-ab323383--western-blot-img441604.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (AB323383)")
![Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (AB323383), expandable thumbnail](https://content.abcam.com/products/images/oncostatin-m-osm-antibody-epr26066-16-ab323383--western-blot-img441603.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (AB323383)")
![Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (AB323383), expandable thumbnail](https://content.abcam.com/products/images/oncostatin-m-osm-antibody-epr26066-16-ab323383--western-blot-img441602.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (AB323383)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Mouse | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity).
Alternative names
Oncostatin-M, OSM, Osm
Rabbit Monoclonal Oncostatin M/OSM antibody. Suitable for WB and reacts with Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR26066-16
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383), expandable thumbnail](https://content.abcam.com/products/images/oncostatin-m-osm-antibody-epr26066-16-ab323383--western-blot-img441604.jpg "Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383)")
Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: 4T1.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 50 µg
Lane 2: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 28 kDa, 124 kDa
Exposure time: 26s
![Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383), expandable thumbnail](https://content.abcam.com/products/images/oncostatin-m-osm-antibody-epr26066-16-ab323383--western-blot-img441603.jpg "Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383)")
Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: skeletal muscle.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383) at 1/1000 dilution
Lane 1: Mouse spleen tissue lysate at 50 µg
Lane 2: Mouse skeletal muscle tissue lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 28 kDa, 36 kDa
Exposure time: 26s
![Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383), expandable thumbnail](https://content.abcam.com/products/images/oncostatin-m-osm-antibody-epr26066-16-ab323383--western-blot-img441602.jpg "Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383)")
Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of Oncostatin-M is upregulated in response to LPS treatment (PMID: 12061841).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Oncostatin M/OSM antibody [EPR26066-16] (ab323383) at 1/1000 dilution
Lane 1: Untreated mouse lung tissue lysate at 50 µg
Lane 2: Mouse lung treated with 1ug/ml LPS and 1ug/ml BFA for 16h tissue lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 28 kDa, 36 kDa
Exposure time: 26s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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