AB317370

Anti-ONECUT2/Oc2 antibody

Name: Anti-ONECUT2/Oc2 antibody (ab317370) | Abcam
Brand: Abcam Limited
SKU: AB317370

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Rabbit Polyclonal ONECUT2/Oc2 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ONECUT2 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Oc2, One cut domain family member 2, One cut homeobox 2, Transcription factor ONECUT-2, OC-2

1 Images

Supplier Data

Western blot - Anti-ONECUT2/Oc2 Antibody (AB317370)

Mouse cerebrum lysate probed with ab317370 at 1 : 300 overnight at 4degC, followed by a conjugated secondary antibody at 1 : 5000 for 90 min at 37degC.

All lanes:

Western blot - Anti-ONECUT2/Oc2 Antibody (ab317370) at 1/300 dilution

All lanes:

mouse cerebrum lysate

Predicted band size: 54554 Da

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human ONECUT2 conjugated to Keyhole Limpet Haemocyanin.

O95948

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/5000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 20% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ONECUT2 also known as Oc2 is a transcription factor that plays a significant role in gene regulation. It belongs to the Onecut family of transcription factors characterized by the presence of an atypical homeodomain and a single-cut domain. ONECUT2 has a molecular weight of around 51 kDa. This protein shows a broad expression across various tissues including liver pancreas and brain and it regulates genes involved in important developmental processes.

Biological function summary

ONECUT2 influences cell differentiation metabolic processes and organogenesis. It does not exist as part of a large complex but interacts with specific DNA sequences to modulate transcription. This protein contributes to proper liver and pancreatic development by activating genes critical for functional maturation. It also has a role in neural development and has been linked with neuronal gene expression patterns.

Pathways

ONECUT2 participates in the regulation of the glucose metabolism and hepatocyte differentiation pathways. Within these pathways ONECUT2 acts in concert with other transcription factors like HNF6 (Hepatocyte Nuclear Factor 6) another member of the Onecut family to regulate gene expression. The protein exerts influence over processes critical for maintaining cellular function and development highlighting its implications in various physiological contexts.

ONECUT2 has connections to cancer and type 2 diabetes. In cancer abnormal expression levels can influence tumor progression and metastasis often linked with the protein’s ability to modulate genes that control cell proliferation and survival. In the context of type 2 diabetes altered ONECUT2 expression affects insulin secretion and glucose homeostasis commonly in conjunction with proteins such as PDX1 (Pancreatic Duodenal Homeobox 1) impacting pancreatic function. Understanding the role of ONECUT2 in these conditions provides valuable insights for therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B (By similarity).

See full target information Onecut2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ONECUT2/Oc2 antibody

Western blot - Anti-ONECUT2/Oc2 Antibody (AB317370)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise