Rabbit Polyclonal ONECUT2/Oc2 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ONECUT2 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 20% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ONECUT2 conjugated to Keyhole Limpet Haemocyanin. Database link O95948
Reactivity data
Select an application| WB | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/300 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Transcriptional activator. Activates the transcription of a number of liver genes such as HNF3B (By similarity).
Alternative names
Oc2, One cut domain family member 2, One cut homeobox 2, Transcription factor ONECUT-2, OC-2
Recommended products
Rabbit Polyclonal ONECUT2/Oc2 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ONECUT2 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 20% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ONECUT2 conjugated to Keyhole Limpet Haemocyanin. Database link O95948
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ONECUT2 also known as Oc2 is a transcription factor that plays a significant role in gene regulation. It belongs to the Onecut family of transcription factors characterized by the presence of an atypical homeodomain and a single-cut domain. ONECUT2 has a molecular weight of around 51 kDa. This protein shows a broad expression across various tissues including liver pancreas and brain and it regulates genes involved in important developmental processes.
- Biological function summary
ONECUT2 influences cell differentiation metabolic processes and organogenesis. It does not exist as part of a large complex but interacts with specific DNA sequences to modulate transcription. This protein contributes to proper liver and pancreatic development by activating genes critical for functional maturation. It also has a role in neural development and has been linked with neuronal gene expression patterns.
- Pathways
ONECUT2 participates in the regulation of the glucose metabolism and hepatocyte differentiation pathways. Within these pathways ONECUT2 acts in concert with other transcription factors like HNF6 (Hepatocyte Nuclear Factor 6) another member of the Onecut family to regulate gene expression. The protein exerts influence over processes critical for maintaining cellular function and development highlighting its implications in various physiological contexts.
- Associated diseases and disorders
ONECUT2 has connections to cancer and type 2 diabetes. In cancer abnormal expression levels can influence tumor progression and metastasis often linked with the protein’s ability to modulate genes that control cell proliferation and survival. In the context of type 2 diabetes altered ONECUT2 expression affects insulin secretion and glucose homeostasis commonly in conjunction with proteins such as PDX1 (Pancreatic Duodenal Homeobox 1) impacting pancreatic function. Understanding the role of ONECUT2 in these conditions provides valuable insights for therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-ONECUT2/Oc2 Antibody (ab317370)
Mouse cerebrum lysate probed with ab317370 at 1:300 overnight at 4degC, followed by a conjugated secondary antibody at 1:5000 for 90 min at 37degC.
All lanes: Western blot - Anti-ONECUT2/Oc2 Antibody (ab317370) at 1/300 dilution
All lanes: mouse cerebrum lysate
Predicted band size: 54554 Da
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com