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AB183879

Anti-OP-2 antibody

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(2 Publications)

Rabbit Polyclonal OP-2 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BMP8B aa 1-300.

View Alternative Names

BMP8, BMP8B, Bone morphogenetic protein 8B, BMP-8, BMP-8B, Osteogenic protein 2, OP-2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-OP-2 antibody (AB183879)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OP-2 antibody (AB183879)

Immunofluorescence analysis of Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling BMP8b protein at cytoplasm using ab183879 at a 1/2000 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

Western blot - Anti-OP-2 antibody (AB183879)
  • WB

Supplier Data

Western blot - Anti-OP-2 antibody (AB183879)

10 % SDS-PAGE

All lanes:

Western blot - Anti-OP-2 antibody (ab183879) at 1/20000 dilution

Lane 1:

293T whole cell lysate

Lane 2:

Human BMP8b-transfected 293T cells whole cell lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human BMP8B aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P34820

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OP-2 also known as "Op protein" or "Anti-Op" is a well-characterized protein with a mass of approximately 45 kDa. This protein is widely expressed in various tissues including the brain liver and spleen. OP-2 has a significant role in several physiological processes through its mechanical functions. As a molecular component it engages in protein interactions that influence cellular communication and signal transduction. Researchers have devoted considerable attention to understanding the complete range of its interactions.
Biological function summary

The protein participates in essential regulatory networks and modulates cellular responses to external stimuli. It often acts as a part of multi-protein complexes that are critical for its function. These complexes facilitate the protein's ability to engage in cell cycle regulation and apoptosis. Additionally OP-2 influences the stability and degradation of certain denatured proteins which emphasizes its role in maintaining cellular homeostasis. The protein's significance extends to acting as a mediator in immune responses.

Pathways

OP-2 plays important roles in both the MAPK/ERK and NF-kB signaling pathways. These pathways are integral to the regulation of cell proliferation differentiation and survival. In the MAPK/ERK pathway OP-2 interacts with a variety of kinases and phosphatases impacting their activity and consequently affecting transcription factors such as c-Myc and Fos. Meanwhile within the NF-kB pathway OP-2 can modulate the activity of IKK-γ which is an important regulatory protein controlling NF-kB activation. Through these interactions OP-2 influences cellular responses to stress and inflammation.

OP-2 shows a strong connection to certain cancers and autoimmune diseases. For instance aberrations in OP-2 function or expression levels have been associated with breast cancer where it affects tumor growth and metastasis. Researchers have also found links between OP-2 and multiple sclerosis a chronic autoimmune condition suggesting its involvement in immune regulation. Other proteins such as TNF-α often intersect with OP-2 in these diseases contributing to disease pathology. Understanding these associations helps in identifying potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (By similarity).
See full target information BMP8B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 13:122-138 PubMed39378431

2024

Complement Factor H Is an ICOS Ligand Modulating Tregs in the Glioma Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina I Smolag,Jakub Olszowka,Rebecca Rosberg,Elinn Johansson,Elisabet Marinko,Karin Leandersson,David J O'Connell,Valeria Governa,Emre Can Tuysuz,Mattias Belting,Alexander Pietras,Myriam Martin,Anna M Blom

Scientific reports 10:21342 PubMed33288848

2020

Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kung-Chao Chang,Ruo-Yu Chen,Yu-Chu Wang,Liang-Yi Hung,L Jeffrey Medeiros,Ya-Ping Chen,Tsai-Yun Chen,Jui-Chu Yang,Po-Min Chiang
View all publications
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Product promise

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