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AB250466

Anti-OP-2 antibody [EPR13357] - BSA and Azide free

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Rabbit Recombinant Monoclonal OP-2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

BMP8, BMP8B, Bone morphogenetic protein 8B, BMP-8, BMP-8B, Osteogenic protein 2, OP-2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-OP-2 antibody [EPR13357] - BSA and Azide free (AB250466)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OP-2 antibody [EPR13357] - BSA and Azide free (AB250466)

This data was developed using ab181253, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of, paraformaldehyde-fixed, A549 cells labeling OP-2 with ab181253 at a 1/250 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200 dilution. In blue DAPI staining.

Western blot - Anti-OP-2 antibody [EPR13357] - BSA and Azide free (AB250466)
  • WB

Supplier Data

Western blot - Anti-OP-2 antibody [EPR13357] - BSA and Azide free (AB250466)

This data was developed using ab181253, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-OP-2 antibody [EPR13357] (<a href='/en-us/products/primary-antibodies/op-2-antibody-epr13357-ab181253'>ab181253</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13357

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects dominant ~35kDa extra band in some samples, e.g. WI-38 and SK-OV-3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250466 is the carrier-free version of ab181253.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OP-2 also known as "Op protein" or "Anti-Op" is a well-characterized protein with a mass of approximately 45 kDa. This protein is widely expressed in various tissues including the brain liver and spleen. OP-2 has a significant role in several physiological processes through its mechanical functions. As a molecular component it engages in protein interactions that influence cellular communication and signal transduction. Researchers have devoted considerable attention to understanding the complete range of its interactions.
Biological function summary

The protein participates in essential regulatory networks and modulates cellular responses to external stimuli. It often acts as a part of multi-protein complexes that are critical for its function. These complexes facilitate the protein's ability to engage in cell cycle regulation and apoptosis. Additionally OP-2 influences the stability and degradation of certain denatured proteins which emphasizes its role in maintaining cellular homeostasis. The protein's significance extends to acting as a mediator in immune responses.

Pathways

OP-2 plays important roles in both the MAPK/ERK and NF-kB signaling pathways. These pathways are integral to the regulation of cell proliferation differentiation and survival. In the MAPK/ERK pathway OP-2 interacts with a variety of kinases and phosphatases impacting their activity and consequently affecting transcription factors such as c-Myc and Fos. Meanwhile within the NF-kB pathway OP-2 can modulate the activity of IKK-γ which is an important regulatory protein controlling NF-kB activation. Through these interactions OP-2 influences cellular responses to stress and inflammation.

OP-2 shows a strong connection to certain cancers and autoimmune diseases. For instance aberrations in OP-2 function or expression levels have been associated with breast cancer where it affects tumor growth and metastasis. Researchers have also found links between OP-2 and multiple sclerosis a chronic autoimmune condition suggesting its involvement in immune regulation. Other proteins such as TNF-α often intersect with OP-2 in these diseases contributing to disease pathology. Understanding these associations helps in identifying potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis (By similarity).
See full target information BMP8B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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