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AB121425

Anti-OPALIN antibody

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(3 Publications)

Rabbit Polyclonal OPALIN antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OPALIN aa 50 to C-terminus.

View Alternative Names

HTMP10, TMEM10, OPALIN, Opalin, Oligodendrocytic myelin paranodal and inner loop protein, Transmembrane protein 10

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPALIN antibody (AB121425)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPALIN antibody (AB121425)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling OPALIN with ab121425. Staining shows moderate cytoplasmic positivity in glial cells.

Western blot - Anti-OPALIN antibody (AB121425)
  • WB

Unknown

Western blot - Anti-OPALIN antibody (AB121425)

All lanes:

Western blot - Anti-OPALIN antibody (ab121425) at 1/250 dilution

Lane 1:

Vector only transfected HEK293T cell lysate

Lane 2:

Lysate from transfected HEK293T cells overexpressing OPALIN with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 16 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human OPALIN aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96PE5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OPALIN also known as Opalin sialomucin or oligodendrocytic myelin paranodal and inner loop protein is a small transmembrane protein with a molecular mass of approximately 11 kDa. Researchers discovered OPALIN in the myelin of the central nervous system specifically expressed in oligodendrocytes. OPALIN functions in cell signaling and is essential for myelin sheath integrity which ensures proper nerve impulse conduction.
Biological function summary

OPALIN is important for oligodendrocyte differentiation and myelination processes critical for the development and maintenance of the central nervous system. OPALIN integrates within the myelin sheath as part of a complex membrane structure assisting in the formation and stabilization of myelin. Its presence is integral for proper myelin development and long-term stability contributing to neuronal function and communication.

Pathways

OPALIN interacts with the oligodendrogenesis pathway which oversees the formation of myelinating cells. In this pathway proteins like Myelin Basic Protein (MBP) and Proteolipid Protein (PLP) are related to OPALIN as they also play pivotal roles in myelin sheath formation and maintenance. OPALIN’s interaction in this context makes it an essential component in ensuring effective myelin repair and function within neural pathways.

OPALIN holds significance for multiple sclerosis (MS) and leukodystrophies. These conditions are characterized by the degeneration or dysfunction of myelin and improper expression or functioning of OPALIN could influence disease progression. In these disorders altered interactions between OPALIN and other myelin-associated proteins like MBP could contribute to pathological outcomes and highlights the importance of maintaining proper OPALIN function for neural health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Central nervous system-specific myelin protein that increase myelin genes expression during oligodendrocyte differentiation. Promotes oligodendrocyte terminal differentiation.
See full target information OPALIN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 11:84 PubMed37217978

2023

Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS function.

Applications

Unspecified application

Species

Unspecified reactive species

Luise A Seeker,Nadine Bestard-Cuche,Sarah Jäkel,Nina-Lydia Kazakou,Sunniva M K Bøstrand,Laura J Wagstaff,Justyna Cholewa-Waclaw,Alastair M Kilpatrick,David Van Bruggen,Mukund Kabbe,Fabio Baldivia Pohl,Zahra Moslehi,Neil C Henderson,Catalina A Vallejos,Gioele La Manno,Goncalo Castelo-Branco,Anna Williams

Bioengineered 12:6643-6654 PubMed34516348

2021

Four specific biomarkers associated with the progression of glioblastoma multiforme in older adults identified using weighted gene co-expression network analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yushi Yang,Liangzhao Chu,Zhirui Zeng,Shu Xu,Hua Yang,Xuelin Zhang,Jun Jia,Niya Long,Yaxin Hu,Jian Liu

Nature 566:543-547 PubMed30747918

2019

Altered human oligodendrocyte heterogeneity in multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Jäkel,Eneritz Agirre,Ana Mendanha Falcão,David van Bruggen,Ka Wai Lee,Irene Knuesel,Dheeraj Malhotra,Charles Ffrench-Constant,Anna Williams,Gonçalo Castelo-Branco
View all publications
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