AB325868

Anti-OPN1MW antibody [HL3435] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal Opn1mw antibody. Carrier free. Suitable for IHC-P, ICC/IF, IHC-Fr, WB and reacts with Mouse, Rat, Transfected cell line - Human, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human OPN1MW.

View Alternative Names

GCP, OPN1MW, Medium-wave-sensitive opsin 1, Green cone photoreceptor pigment, Green-sensitive opsin, GOP, Medium-wave-sensitive opsin 2, Green cone photoreceptor pigment, Green-sensitive opsin, Opsin 1 cone pigments medium-wave-sensitive 2, GOP

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

ab325868 detects OPN1MW2 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat eye. Green : OPN1MW2 stained by ab325868 diluted at 1 : 250. Red : beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Frozen sections) - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

ab325868 detects OPN1MW2 protein by immunohistochemical analysis. Sample : Frozen-sectioned mouse eye. Green : OPN1MW2 stained by ab325868 diluted at 1 : 250. Red : beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

ab325868 detects OPN1MW2 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse eye. Green : OPN1MW2 stained by ab325868 diluted at 1 : 250. Red : beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

ab325868 detects OPN1MW2 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : OPN1MW2 stained by ab325868 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Supplier Data

Western blot - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (AB325868)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325868 diluted at 1 : 3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-OPN1MW antibody [HL3435] - BSA and Azide free (ab325868) at 1/3000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged OPN1MW2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged OPN1MW2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL3435

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

ICC/IF, IHC-P, IHC-Fr, WB

applications

Immunogen

Recombinant Fragment Protein within Human OPN1MW.

P04001

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.

See full target information OPN1MW

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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