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AB155763

Anti-OPRT antibody

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(3 Publications)

Rabbit Polyclonal UMPS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UMPS aa 200-500.

View Alternative Names

OK/SW-cl.21, UMPS, Uridine 5'-monophosphate synthase, UMP synthase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-OPRT antibody (AB155763)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-OPRT antibody (AB155763)

Immunofluorescence analysis of methanol-fixed HeLa cells labeling OPRT with ab155763 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPRT antibody (AB155763)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPRT antibody (AB155763)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling OPRT with ab155763 at 1/500 dilution.

Western blot - Anti-OPRT antibody (AB155763)
  • WB

Unknown

Western blot - Anti-OPRT antibody (AB155763)

10% SDS PAGE

All lanes:

Western blot - Anti-OPRT antibody (ab155763) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human UMPS aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

P11172

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orotate phosphoribosyltransferase often abbreviated as OPRT or known by its enzyme commission number EC 2.4.2.10 plays a significant role in pyrimidine biosynthesis. This enzyme with a molecular weight of around 23 kDa catalyzes the conversion of orotate and 5-phosphoribosyl-1-pyrophosphate (PRPP) into orotidine-5'-monophosphate (OMP). OPRT is expressed in various tissues with notably higher levels in rapidly proliferating cells due to their increased demand for nucleotides.
Biological function summary

Orotate phosphoribosyltransferase contributes to the de novo synthesis of pyrimidine nucleotides which are essential components of DNA and RNA. OPRT operates as part of the UMP biosynthesis via the de novo pathway forming a complex with orotidine-5'-phosphate decarboxylase in some organisms. This linkage enhances efficiency in pyrimidine nucleotide production reflecting its central role in cell growth and division.

Pathways

The protein integrates into the pyrimidine metabolism pathway. It interacts closely with orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase forming dual-function enzymes that streamline UMP synthesis. OPRT’s activity ties it to key metabolic cycles including nucleotide salvage pathways thereby establishing critical links across metabolic networks essential for nucleic acid production.

Dysfunction in Orotate phosphoribosyltransferase can lead to hereditary orotic aciduria a rare autosomal recessive disorder characterized by megaloblastic anemia and excessive orotate excretion. Changes in OPRT activity also impact pyrimidine antimetabolite drug efficacy such as 5-fluorouracil demonstrating its significance in cancer treatment. Additionally OPRT's interactions with dihydroorotate dehydrogenase can be important in understanding metabolic disturbances related to pyrimidine disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis, orotate phosphoribosyltransferase (OPRT), which converts orotate to orotidine-5'-monophosphate (OMP), and orotidine-5'-monophosphate decarboxylase (ODC), the terminal enzymatic reaction that decarboxylates OMP to uridine monophosphate (UMP).
See full target information UMPS
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2022:2434938 PubMed36299824

2022

LINC01140 Targeting miR-452-5p/RGS2 Pathway to Attenuate Breast Cancer Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoshi Li

Oncology reports 45: PubMed33649846

2021

Mechanism of acquired 5FU resistance and strategy for overcoming 5FU resistance focusing on 5FU metabolism in colon cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonari Suetsugu,Ryutaro Mori,Manabu Futamura,Masahiro Fukada,Hideharu Tanaka,Itaru Yasufuku,Yuta Sato,Yoshinori Iwata,Takeharu Imai,Hisashi Imai,Yoshihiro Tanaka,Naoki Okumura,Nobuhisa Matsuhashi,Takao Takahashi,Kazuhiro Yoshida

Nature methods 17:311-318 PubMed32015544

2020

An optimized chemical-genetic method for cell-specific metabolic labeling of RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Nainar,Bonnie J Cuthbert,Nathan M Lim,Whitney E England,Ke Ke,Kanika Sophal,Robert Quechol,David L Mobley,Celia W Goulding,Robert C Spitale
View all publications
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