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AB234676

Anti-OPRT antibody

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Rabbit Polyclonal PYRE antibody. Suitable for WB and reacts with Recombinant full length protein - Laribacter hongkongensis samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Laribacter hongkongensis HLHK9 pyrE.

View Alternative Names

LHK_03212, pyrE, Orotate phosphoribosyltransferase, OPRT, OPRTase

1 Images
Western blot - Anti-OPRT antibody (AB234676)
  • WB

Supplier Data

Western blot - Anti-OPRT antibody (AB234676)

All lanes:

Western blot - Anti-OPRT antibody (ab234676) at 1/500 dilution

Lane 1:

Recombinant Laribacter hongkongensis OPRT protein, 80 ng

Lane 2:

Recombinant Laribacter hongkongensis OPRT protein, 40 ng

Lane 3:

Recombinant Laribacter hongkongensis OPRT protein, 20 ng

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 52 kDa

Observed band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Laribacter hongkongensis HLHK9 pyrE.

C1D6F5

Specificity

Predicted to react with Laribacter hongkongensis.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Laribacter hongkongensis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orotate phosphoribosyltransferase often abbreviated as OPRT or known by its enzyme commission number EC 2.4.2.10 plays a significant role in pyrimidine biosynthesis. This enzyme with a molecular weight of around 23 kDa catalyzes the conversion of orotate and 5-phosphoribosyl-1-pyrophosphate (PRPP) into orotidine-5'-monophosphate (OMP). OPRT is expressed in various tissues with notably higher levels in rapidly proliferating cells due to their increased demand for nucleotides.
Biological function summary

Orotate phosphoribosyltransferase contributes to the de novo synthesis of pyrimidine nucleotides which are essential components of DNA and RNA. OPRT operates as part of the UMP biosynthesis via the de novo pathway forming a complex with orotidine-5'-phosphate decarboxylase in some organisms. This linkage enhances efficiency in pyrimidine nucleotide production reflecting its central role in cell growth and division.

Pathways

The protein integrates into the pyrimidine metabolism pathway. It interacts closely with orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase forming dual-function enzymes that streamline UMP synthesis. OPRT's activity ties it to key metabolic cycles including nucleotide salvage pathways thereby establishing critical links across metabolic networks essential for nucleic acid production.

Dysfunction in Orotate phosphoribosyltransferase can lead to hereditary orotic aciduria a rare autosomal recessive disorder characterized by megaloblastic anemia and excessive orotate excretion. Changes in OPRT activity also impact pyrimidine antimetabolite drug efficacy such as 5-fluorouracil demonstrating its significance in cancer treatment. Additionally OPRT's interactions with dihydroorotate dehydrogenase can be important in understanding metabolic disturbances related to pyrimidine disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).
See full target information pyrE
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