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AB151240

Anti-Optineurin antibody

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(4 Publications)

Rabbit Polyclonal Optineurin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OPTN aa 150 to C-terminus.

View Alternative Names

FIP2, GLC1E, HIP7, HYPL, NRP, OPTN, Optineurin, E3-14.7K-interacting protein, Huntingtin yeast partner L, Huntingtin-interacting protein 7, Huntingtin-interacting protein L, NEMO-related protein, Optic neuropathy-inducing protein, Transcription factor IIIA-interacting protein, FIP-2, HIP-7, TFIIIA-IntP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Optineurin antibody (AB151240)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Optineurin antibody (AB151240)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling Optineurin with ab151240 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Optineurin antibody (AB151240)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Optineurin antibody (AB151240)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Optineurin with ab151240 at 1/500 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human OPTN aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96CV9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Optineurin also known as OPTN is a multifunctional protein of approximately 67 kDa. It is expressed in various tissues including the retina where it plays a role in maintaining cellular functions. Optineurin interacts with several binding partners to execute its roles within the cell. It is involved in intracellular trafficking signaling and inflammation processes. As an essential component of the cellular machinery optineurin aids in proper cellular communication and responses.
Biological function summary

Optineurin influences different cellular processes notably autophagy and regulation of inflammation. It participates in forming a complex with other proteins to facilitate cargo transport and degradation via the autophagy-lysosome pathway. This function is important for removing damaged proteins and organelles ensuring cellular homeostasis. Inflammation regulation occurs through optineurin's ability to inhibit certain signaling pathways therefore modulating immune responses and preventing excessive inflammation.

Pathways

Optineurin plays a role in the NF-kB signaling pathway and the autophagic process. In NF-kB signaling it acts as a negative regulator by degrading specific signaling molecules. This regulation is necessary to prevent chronic inflammation and maintain immune balance. Within autophagy optineurin collaborates with other autophagy-related proteins like LC3 to facilitate the sequestration and clearance of cellular components preventing accumulation of damaged cellular materials.

Optineurin connects to conditions like glaucoma and amyotrophic lateral sclerosis (ALS). Mutations in the optineurin gene link to glaucoma as they can impair autophagy and lead to optic nerve damage. In the context of ALS optineurin mutations disrupt normal cellular functions contributing to motor neuron degeneration. The protein TDP-43 is often associated with ALS and interacts with optineurin in pathological processes affecting disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8 (PubMed : 27534431). Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation (PubMed : 27534431). Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation (PubMed : 27538435). In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta/IFNB1. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and huntingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as that of transferrin receptor (TFRC/TfR); regulates Rab8 recruitment to tubules emanating from the endocytic recycling compartment (PubMed : 22854040). Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52.. (Microbial infection) May constitute a cellular target for various viruses, such as adenovirus E3 14.7 or Bluetongue virus, to inhibit innate immune response (PubMed : 27538435, PubMed : 9488477). During RNA virus infection, such as that of Sendai virus, negatively regulates the induction of IFNB1 (PubMed : 20174559).
See full target information OPTN
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 22:628 PubMed35672728

2022

Interfering with the expression of EEF1D gene enhances the sensitivity of ovarian cancer cells to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Qia Xu,Yun Liu,Shenyi Wang,Jing Wang,Liwei Liu,Yin Xu,Yide Qin

Translational psychiatry 12:230 PubMed35662233

2022

OPTN attenuates the neurotoxicity of abnormal Tau protein by restoring autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Xu,Yun Liu,Xi Chen,Qia Xu,Liwei Liu,Hui Liu,Ruowen Guo,Yide Qin

Cells 9: PubMed32121108

2020

Motor Neuron Generation from iPSCs from Identical Twins Discordant for Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Emily R Seminary,Stephanie Santarriaga,Lynn Wheeler,Marie Mejaki,Jenica Abrudan,Wendy Demos,Michael T Zimmermann,Raul A Urrutia,Dominic Fee,Paul E Barkhaus,Allison D Ebert

Frontiers in neuroscience 12:86 PubMed29515358

2018

Modeling Protein Aggregation and the Heat Shock Response in ALS iPSC-Derived Motor Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Emily R Seminary,Samantha L Sison,Allison D Ebert
View all publications
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Product promise

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