Rabbit Polyclonal ORAI2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ORAI2 aa 1-250.
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Publications
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- Journal of hypertension 36:377-3862018Essential role for smooth muscle cell stromal interaction molecule-1 in myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVishal Mali,Samuel Haddox,Souad Belmadani,Khalid MatrouguiPubMed 29611835
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ORAI2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96SN7
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Pore-forming subunit of inward rectifying Ca(2+) release-activated Ca(2+) (CRAC) channels. Assembles with ORAI1 and ORAI3 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. CRAC channels are the main pathway for Ca(2+) influx in T cells and promote the immune response to pathogens by activating NFAT-dependent cytokine and chemokine transcription.
Alternative names
C7orf19, CBCIP2, TMEM142B, PP1729, ORAI2, Protein orai-2, CAP-binding protein complex-interacting protein 2, Transmembrane protein 142B
Recommended products
Rabbit Polyclonal ORAI2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ORAI2 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ORAI2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96SN7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Orai2 also known as Orai calcium-release-activated calcium modulator 2 is a protein that functions as a calcium channel subunit. It plays an important role in regulating calcium influx into cells. Orai2 is a transmembrane protein expressed in various tissues including the brain and muscle. The protein has a mass of approximately 33 kDa. Orai2 forms part of a channel complex important for controlling the amount of calcium that enters cells in response to signals.
- Biological function summary
Orai2 modulates calcium homeostasis by forming a complex with other proteins in the Orai family such as Orai1 and Orai3. Calcium ions are essential for numerous cellular processes including muscle contraction neurotransmitter release and gene expression. Orai2 helps to fine-tune the calcium signaling pathways that decide these processes. By collaborating with other calcium channels and regulatory proteins Orai2 adjusts the cellular calcium influx that tackles specific cellular needs.
- Pathways
Calcium release-activated calcium (CRAC) channels represent an important biological route in which Orai2 participates. The CRAC channel mechanism involves the interaction with stromal interaction molecule 1 (STIM1) a protein that senses calcium depletion in the endoplasmic reticulum. Orai2 when associated with STIM1 conducts calcium ions into the cytosol maintaining intracellular calcium levels required for various cellular responses. Additionally Orai2 interacts within pathways that involve Orai1 and STIM-like protein 1 (STIM2) highlighting its role in regulating cellular calcium mechanisms.
- Associated diseases and disorders
Orai2 relates to immune system dysfunctions and neurological conditions. Abnormal alterations in Orai2 function can contribute to immunodeficiency as calcium entry through Orai channels influences immune cell activation and response. Additionally alteration in Orai2 function can affect neuronal signaling having potential links to conditions like epilepsy. Orai2's function in these disorders may involve interactions with related proteins such as Orai1 and STIM1 which also play a part in these pathways. Understanding Orai2's role offers potential avenues for treating related conditions by targeting calcium entry mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Orai2 antibody (ab155216)
12% SDS PAGE
All lanes: Western blot - Anti-Orai2 antibody (ab155216) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai2 antibody (ab155216)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Orai2 with ab155216 at 1/500 dilution.
Western blot - Anti-Orai2 antibody (ab155216)
12% SDS-PAGE
All lanes: Western blot - Anti-Orai2 antibody (ab155216) at 1/500 dilution
Lane 1: Mouse brain tissue extract at 50 µg
Lane 2: Rat brain tissue extract at 50 µg
Predicted band size: 29 kDa
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