AB180146

Anti-Orai2 antibody [EPR10043(2)] - C-terminal

Name: Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146) | Abcam
Brand: Abcam Limited
SKU: AB180146
Price: 460 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal ORAI2 antibody. C-terminal. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

C7orf19, CBCIP2, TMEM142B, PP1729, ORAI2, Protein orai-2, CAP-binding protein complex-interacting protein 2, Transmembrane protein 142B

2 Images

Flow Cytometry (Intracellular) - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (AB180146)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (AB180146)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Orai2 with ab180146 at 1/200 dilution (red). Secondary antibody Goat anti rabbit IgG (FITC) at 1/75 dilution. Isotype control Rabbit IgG (green).

Supplier Data

Western blot - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (AB180146)

All lanes:

Western blot - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (ab180146) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MDA-MB-435 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 29 kDa

false

Carrier free

Anti-Orai2 antibody [EPR10043(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10043(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orai2 also known as Orai calcium-release-activated calcium modulator 2 is a protein that functions as a calcium channel subunit. It plays an important role in regulating calcium influx into cells. Orai2 is a transmembrane protein expressed in various tissues including the brain and muscle. The protein has a mass of approximately 33 kDa. Orai2 forms part of a channel complex important for controlling the amount of calcium that enters cells in response to signals.

Biological function summary

Orai2 modulates calcium homeostasis by forming a complex with other proteins in the Orai family such as Orai1 and Orai3. Calcium ions are essential for numerous cellular processes including muscle contraction neurotransmitter release and gene expression. Orai2 helps to fine-tune the calcium signaling pathways that decide these processes. By collaborating with other calcium channels and regulatory proteins Orai2 adjusts the cellular calcium influx that tackles specific cellular needs.

Pathways

Calcium release-activated calcium (CRAC) channels represent an important biological route in which Orai2 participates. The CRAC channel mechanism involves the interaction with stromal interaction molecule 1 (STIM1) a protein that senses calcium depletion in the endoplasmic reticulum. Orai2 when associated with STIM1 conducts calcium ions into the cytosol maintaining intracellular calcium levels required for various cellular responses. Additionally Orai2 interacts within pathways that involve Orai1 and STIM-like protein 1 (STIM2) highlighting its role in regulating cellular calcium mechanisms.

Orai2 relates to immune system dysfunctions and neurological conditions. Abnormal alterations in Orai2 function can contribute to immunodeficiency as calcium entry through Orai channels influences immune cell activation and response. Additionally alteration in Orai2 function can affect neuronal signaling having potential links to conditions like epilepsy. Orai2's function in these disorders may involve interactions with related proteins such as Orai1 and STIM1 which also play a part in these pathways. Understanding Orai2's role offers potential avenues for treating related conditions by targeting calcium entry mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming subunit of inward rectifying Ca(2+) release-activated Ca(2+) (CRAC) channels. Assembles with ORAI1 and ORAI3 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. CRAC channels are the main pathway for Ca(2+) influx in T cells and promote the immune response to pathogens by activating NFAT-dependent cytokine and chemokine transcription.

See full target information ORAI2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 26:503 PubMed37822583

2023

Downregulation of lncRNA SATB2‑AS1 facilitates glioma cell proliferation by sponging miR‑671‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Gu,Yongqing Ye,Rauniyar Sunil,Wenjian Zhan,Rutong Yu

The Journal of biological chemistry 298:102681 PubMed36356899

2022

MITF is a novel transcriptional regulator of the calcium sensor STIM1: Significance in physiological melanogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jyoti Tanwar,Akshay Sharma,Suman Saurav, Shyamveer,Nidhi Jatana,Rajender K Motiani

Cancers 13: PubMed34885048

2021

Orai3 Regulates Pancreatic Cancer Metastasis by Encoding a Functional Store Operated Calcium Entry Channel.

Applications

Unspecified application

Species

Unspecified reactive species

Samriddhi Arora,Jyoti Tanwar,Nutan Sharma,Suman Saurav,Rajender K Motiani

iScience 24:103339 PubMed34816101

2021

Resolving macrophage polarization through distinct Ca entry channel that maintains intracellular signaling and mitochondrial bioenergetics.

Applications

Unspecified application

Species

Unspecified reactive species

Viviane Nascimento Da Conceicao,Yuyang Sun,Karthik Ramachandran,Arun Chauhan,Amritha Raveendran,Manigandan Venkatesan,Bony DeKumar,Soumya Maity,Neelanjan Vishnu,George A Kotsakis,Paul F Worley,Donald L Gill,Bibhuti B Mishra,Muniswamy Madesh,Brij B Singh

Frontiers in immunology 12:779560 PubMed34745151

2021

CRAC Channel Controls the Differentiation of Pathogenic B Cells in Lupus Nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Li,Qin Zeng,Shuyi Wang,Mengyuan Li,Xionghui Chen,Yuefang Huang,Binfeng Chen,Mianjing Zhou,Yimei Lai,Chaohuan Guo,Siyuan Zhao,Hui Zhang,Niansheng Yang

Nature communications 11:5489 PubMed33127885

2020

Crbn modulates calcium influx by regulating Orai1 during efferocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunji Moon,Chanhyuk Min,Gayoung Kim,Deokhwan Kim,Kwanhyeong Kim,Sang-Ah Lee,Byeongjin Moon,Susumin Yang,Juyeon Lee,Seung-Joo Yang,Steve K Cho,Gwangrog Lee,Chang Sup Lee,Chul-Seung Park,Daeho Park

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Orai2 antibody [EPR10043(2)] - C-terminal

Flow Cytometry (Intracellular) - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (AB180146)

Western blot - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (AB180146)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise