JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB255336

Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ORAI3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples.

View Alternative Names

TMEM142C, ORAI3, Protein orai-3, Transmembrane protein 142C

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human colon cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID : 30123430 ) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human breast cancer tissue (A) while weak staining in the adjacent noncancerous tissue (B) (PMID : 23266555) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID : 24058448 ) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (AB255336)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse colon (PMID : 30123430 ) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22575-17

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB224095

Anti-Orai3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Orai3 antibody (AB224095)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB180146

Anti-Orai2 antibody [EPR10043(2)] - C-terminal

RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-Orai2 antibody [EPR10043(2)] - C-terminal (AB180146)

  • 5
  • 1
Primary Antibodies

AB303565

Anti-CD68 antibody [RM1031]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-CD68 antibody [RM1031] (AB303565)

  • 0
  • 1
Primary Antibodies

AB108994

Anti-Stromal interaction molecule 1 antibody [EPR3414]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stromal interaction molecule 1 antibody [EPR3414] (AB108994)

  • 5
  • 1
Primary Antibodies

AB221144

Anti-TRP2/DCT antibody [EPR21986]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (AB221144)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "10.82 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1.082", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "10.82 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab255336 is the carrier-free version of ab254260.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orai3 is a protein known as a part of the calcium release-activated calcium (CRAC) channel family. It is also referred to as Orai calendar in certain contexts. This protein serves as a critical component in the highly selective process of calcium ion entry across the plasma membrane. Orai3 has a mass of approximately 33 kDa. It is expressed predominantly in tissues like the pancreas lung and immune system which often require tight regulation of calcium homeostasis for their proper function.
Biological function summary

Orai3 plays an essential role in cellular calcium signaling important for various cell functions such as gene expression secretion and metabolism. It often combines with other subunits of the Orai family like Orai1 to form functional calcium channels. Orai3 may form different channel complexes affecting the amplitude and kinetics of calcium entry. Its presence ensures that cells can adapt to specific physiological contexts where calcium influx is required.

Pathways

Orai3 is a significant part of the store-operated calcium entry (SOCE) pathway which activates when intracellular calcium stores get depleted. This pathway is important for maintaining cellular calcium and proteins like STIM1 work closely with Orai3 to initiate calcium influx. The interplay between STIM1 and Orai3 ensures proper signal transduction essential for processes like T-cell activation and other immune responses.

Orai3 has implications in certain cancers and cardiovascular diseases. In some cancer types like breast cancer overexpression of Orai3 contributes to cell proliferation and survival which can lead to tumor progression. Similarly in cardiovascular diseases altered function or expression of Orai3 may impact calcium regulation leading to issues such as hypertension. Its association with STIM1 in these disorders highlights the cooperative role they play in maintaining cellular health emphasizing the importance of regulated calcium influx in normal and disease states.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pore-forming subunit of two major inward rectifying Ca(2+) channels at the plasma membrane : Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels (PubMed : 16807233, PubMed : 17442569, PubMed : 19182790, PubMed : 19622606, PubMed : 19706554, PubMed : 20354224, PubMed : 32415068). Assembles with ORAI1 and ORAI2 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. CRAC channels are the main pathway for Ca(2+) influx in T cells and promote the immune response to pathogens by activating NFAT-dependent cytokine and chemokine transcription (PubMed : 16807233, PubMed : 17442569, PubMed : 19182790, PubMed : 19706554, PubMed : 20354224, PubMed : 32415068). Assembles with ORAI1 to form channels that mediate store-independent Ca(2+) influx in response to inflammatory metabolites arachidonate or its derivative leukotriene C4, termed ARC and LRC channels respectively (PubMed : 19622606, PubMed : 32415068).
See full target information ORAI3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com