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AB251776

Anti-ORC1 antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal ORC1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ORC1 aa 500-650.

View Alternative Names

ORC1L, PARC1, ORC1, Origin recognition complex subunit 1, Replication control protein 1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-ORC1 antibody (AB251776)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ORC1 antibody (AB251776)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ORC1 (green) using ab251776 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for ORC1 with ab251776 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)

Formalin-fixed, paraffin-embedded human testis tissue stained for ORC1 with ab251776 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for ORC1 with ab251776 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC1 antibody (AB251776)

Formalin-fixed, paraffin-embedded human placenta tissue stained for ORC1 with ab251776 at a 1/1000 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ORC1 aa 500-650. The exact immunogen used to generate this antibody is proprietary information.

Q13415

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25000-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORC1 also known as Origin Recognition Complex subunit 1 is an important component involved in the initiation of DNA replication. It forms part of a larger protein complex that plays a role in recognizing the starting points or origins for replication to begin. ORC1 has a molecular mass of approximately 97 kDa and is expressed in various cell types throughout the body but is especially prominent in cells that actively divide. Its expression ensures that the replication machinery assembles correctly during the cell cycle.
Biological function summary

In terms of chromosomal DNA replication ORC1 plays a central role in forming the pre-replicative complex. This protein is a component of the ORC complex which includes several other subunits responsible for facilitating replication initiation across eukaryotic cells. ORC1 through interactions with additional proteins like CDC6 and CDT1 helps load the MCM helicase onto DNA which is essential for DNA unwinding before replication.

Pathways

The replication origins and ORC1's role in them tie it to the DNA replication and cell cycle pathways. In the DNA replication pathway ORC1 serves as the initial marker for DNA segment to act as a template for the replication machinery. Additionally its relationship with CDC6 links it to the cell cycle regulatory pathways orchestrating timely DNA replication to ensure that cell division occurs accurately and efficiently.

Defects or dysregulation in ORC1 are linked to Meier-Gorlin syndrome a condition characterized by growth delays and developmental abnormalities. Further irregularities in ORC1 function can also contribute to certain cancers due to uncontrolled cell division. Within these disorders connections between ORC1 and proteins such as CDT1 emphasize its role in ensuring orderly cell cycle progression whose disruption can lead to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.
See full target information ORC1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:5035-5054 PubMed36205357

2022

Systemic analysis of the DNA replication regulator origin recognition complex in lung adenocarcinomas identifies prognostic and expression significance.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tang,Juan Chen,Tian Zeng,Dong-Mei Ye,Yu-Kun Li,Juan Zou,Yu-Ping Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com