Goat Polyclonal ORC4L antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human ORC4 aa 400 to C-terminus.
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Publications
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- Molecular cell 81:1951-1969.e62021Multiple, short protein binding motifs in ORC1 and CDC6 control the initiation of DNA replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManzar Hossain,Kuhulika Bhalla,Bruce StillmanPubMed 33761311
- Cell cycle (Georgetown, Tex.) 18:605-6202019Human NOC3 is essential for DNA replication licensing in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan-Hei Cheung,Aftab Amin,Rentian Wu,Yan Qin,Lan Zou,Zhiling Yu,Chun LiangPubMed 30741601
- Oncotarget 7:80190-802072016Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing.Applications:ICC/IFReactive species:HumanRuowen Zhang,Jiahui Wu,Sylvain Ferrandon,Katie J Glowacki,Janet A HoughtonPubMed 27863397
- eLife 5:2016Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription.
- PloS one 10:e01284972015Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.Applications:WBReactive species:Unspecified reactive speciesVladimir V Sherstyuk et. al.PubMed 26038842
- The Journal of biological chemistry 287:15024-332012Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing.
- The Journal of biological chemistry 286:23831-412011Assembly of the human origin recognition complex occurs through independent nuclear localization of its components.
- The Journal of biological chemistry 283:2265-742007FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.Applications:WBReactive species:HumanYoshikazu Johmura,Shigehiro Osada,Makoto Nishizuka,Masayoshi ImagawaPubMed 18029353
- The Journal of biological chemistry 281:23264-732006Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIlian Radichev et. al.PubMed 16762929
- Molecular cell 19:567-752005Human origins of DNA replication selected from a library of nascent DNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVesna Todorovic,Sara Giadrossi,Cristina Pelizon,Ramiro Mendoza-Maldonado,Hisao Masai,Mauro GiaccaPubMed 16109380
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human ORC4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43929
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Cow | Predicted |
| Dog | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-2 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 0.5-2 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 0.5-2 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Alternative names
ORC4L, ORC4, Origin recognition complex subunit 4
Recommended products
Goat Polyclonal ORC4L antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human ORC4 aa 400 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human ORC4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43929
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The origin recognition complex subunit 4-like (ORC4L) also known simply as ORC4 is a mechanically important player in the initiation of DNA replication. ORC4 typically has a molecular mass of approximately 50 kDa. It is highly expressed in cells that are actively dividing especially within the nucleus where replication origins are located. The protein forms part of the larger origin recognition complex (ORC) which is important for marking the starting points for DNA replication across eukaryotic organisms.
- Biological function summary
ORC4 plays a central role in recognizing replication origins and acts as an anchor for the assembly of other necessary replication proteins. It is one of six subunits that comprise the ORC complex. This protein complex binds to origins of replication in DNA unwinds the double helix and recruits additional factors required for the formation of the pre-replicative complex. The presence of ORC4 is essential in ensuring that DNA replication occurs once and only once per cell cycle preventing genomic instability.
- Pathways
Several cellular processes depend on the correct functioning of ORC4. In cell cycle regulation ORC4 is integral to the DNA replication checkpoint which ensures DNA is not damaged before it is duplicated. ORC4 interacts with other replication factors like CDC6 and MCM2-7 helicase to initiate and regulate the replication process. Its activity influences key pathways such as the p53-dependent DNA damage response where ORC4 can affect decisions related to cell cycle arrest or apoptosis.
- Associated diseases and disorders
ORC4 dysregulation links to genomic instability which is a hallmark in the development of cancer. Altered levels or mutations in ORC4 contribute to an increased susceptibility to oncogenesis with links to various cancers such as those of the breast and colon. The BRCA1 protein known for its role in maintaining genomic stability and DNA repair often shows interactions with ORC4 during such disease states highlighting their interconnected roles in cancer-related pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ORC4L antibody (ab9641)
All lanes: Western blot - Anti-ORC4L antibody (ab9641) at 0.5 µg/mL
All lanes: Mouse heart lysate at 35 µg
Predicted band size: 50 kDa
Western blot - Anti-ORC4L antibody (ab9641)
All lanes: Western blot - Anti-ORC4L antibody (ab9641) at 0.5 µg/mL
All lanes: Rat Heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-ORC4L antibody (ab9641)
ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
All lanes: Western blot - Anti-ORC4L antibody (ab9641)
Predicted band size: 50 kDa
Western blot - Anti-ORC4L antibody (ab9641)
All lanes: Western blot - Anti-ORC4L antibody (ab9641) at 1 µg/mL
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 38 kDa, 49 kDa
Exposure time: 20min
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