Rabbit Polyclonal ORC6L antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ORC6 aa 150 to C-terminus.
Images
Publications
Filter by
- Frontiers in cell and developmental biology 9:6522922021ORC6, Negatively Regulated by miR-1-3p, Promotes Proliferation, Migration, and Invasion of Hepatocellular Carcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHu Chen,Lequn Bao,Jianhua Hu,Dongde Wu,Xianli TongPubMed 34395415
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ORC6 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5N6
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Does not bind histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Alternative names
ORC6L, ORC6, Origin recognition complex subunit 6
Recommended products
Rabbit Polyclonal ORC6L antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ORC6 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ORC6 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5N6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ORC6 also known as Origin Recognition Complex Subunit 6 is a fundamental protein in DNA replication. With a molecular mass of about 30 kDa ORC6 is a part of the larger Origin Recognition Complex (ORC) which initiates DNA replication. This protein expresses both in the nucleus and cytoplasm with higher levels in proliferating cells. ORC6's main role involves binding to replication origins helping to recruit other replication factors needed to start DNA replication.
- Biological function summary
ORC6 plays a critical role in the initiation of DNA replication by being part of the Origin Recognition Complex which contains six different ORC subunits. The ORC complex marks the chromosomal sites where the replication process starts. The ORC6 subunit supports the loading of additional essential replication factors such as CDC6 and CDT1 onto DNA. This action helps stabilize the pre-replicative complex allowing for proper DNA synthesis in the S phase of the cell cycle.
- Pathways
ORC6 acts closely within the cell cycle regulation and DNA replication pathways. These pathways are essential for effective cell division and genomic stability. ORC6 works alongside other ORC subunits and proteins like MCM2-7 helicase which unwinds DNA for replication. The close interaction between ORC6 and the CDC6 protein ensures the successful transition from DNA replication initiation to elongation.
- Associated diseases and disorders
Aberrant ORC6 function links to cancer and genomic instability. Cancer often shows overexpression of ORC6 contributing to uncontrolled cell proliferation. Deficiencies in ORC6 can lead to replication stress and genome instability disorders sometimes involving interactions with proteins like CDC6 and CDT1 which further highlight its importance to maintaining genomic fidelity. Understanding ORC6's role in disease can inform potential therapeutic strategies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-ORC6 antibody - C-terminal (ab153993)
12% SDS PAGE
All lanes: Western blot - Anti-ORC6 antibody - C-terminal (ab153993) at 1/1000 dilution
All lanes: MOLT4 whole cell lysate at 30 µg
Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC6 antibody - C-terminal (ab153993)
Immunohistochemical analysis of paraffin-embedded Human TOV-21G xenograft tissue labeling ORC6 with ab153993 at 1/500 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com