JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB153993

Anti-ORC6 antibody - C-terminal

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal ORC6L antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ORC6 aa 150 to C-terminus.

View Alternative Names

ORC6L, ORC6, Origin recognition complex subunit 6

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC6 antibody - C-terminal (AB153993)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC6 antibody - C-terminal (AB153993)

Immunohistochemical analysis of paraffin-embedded Human TOV-21G xenograft tissue labeling ORC6 with ab153993 at 1/500 dilution.

Western blot - Anti-ORC6 antibody - C-terminal (AB153993)
  • WB

Unknown

Western blot - Anti-ORC6 antibody - C-terminal (AB153993)

12% SDS PAGE

All lanes:

Western blot - Anti-ORC6 antibody - C-terminal (ab153993) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ORC6 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5N6

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORC6 also known as Origin Recognition Complex Subunit 6 is a fundamental protein in DNA replication. With a molecular mass of about 30 kDa ORC6 is a part of the larger Origin Recognition Complex (ORC) which initiates DNA replication. This protein expresses both in the nucleus and cytoplasm with higher levels in proliferating cells. ORC6's main role involves binding to replication origins helping to recruit other replication factors needed to start DNA replication.
Biological function summary

ORC6 plays a critical role in the initiation of DNA replication by being part of the Origin Recognition Complex which contains six different ORC subunits. The ORC complex marks the chromosomal sites where the replication process starts. The ORC6 subunit supports the loading of additional essential replication factors such as CDC6 and CDT1 onto DNA. This action helps stabilize the pre-replicative complex allowing for proper DNA synthesis in the S phase of the cell cycle.

Pathways

ORC6 acts closely within the cell cycle regulation and DNA replication pathways. These pathways are essential for effective cell division and genomic stability. ORC6 works alongside other ORC subunits and proteins like MCM2-7 helicase which unwinds DNA for replication. The close interaction between ORC6 and the CDC6 protein ensures the successful transition from DNA replication initiation to elongation.

Aberrant ORC6 function links to cancer and genomic instability. Cancer often shows overexpression of ORC6 contributing to uncontrolled cell proliferation. Deficiencies in ORC6 can lead to replication stress and genome instability disorders sometimes involving interactions with proteins like CDC6 and CDT1 which further highlight its importance to maintaining genomic fidelity. Understanding ORC6's role in disease can inform potential therapeutic strategies for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Does not bind histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
See full target information ORC6
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:5035-5054 PubMed36205357

2022

Systemic analysis of the DNA replication regulator origin recognition complex in lung adenocarcinomas identifies prognostic and expression significance.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tang,Juan Chen,Tian Zeng,Dong-Mei Ye,Yu-Kun Li,Juan Zou,Yu-Ping Zhang

Frontiers in cell and developmental biology 9:652292 PubMed34395415

2021

ORC6, Negatively Regulated by miR-1-3p, Promotes Proliferation, Migration, and Invasion of Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Chen,Lequn Bao,Jianhua Hu,Dongde Wu,Xianli Tong
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB9641

Anti-ORC4L antibody

Western blot - Anti-ORC4L antibody (AB9641)

  • 0
  • 0
Primary Antibodies

AB88756

Anti-ZCCHC2 antibody

Western blot - Anti-ZCCHC2 antibody (AB88756)

  • 0
  • 0
Primary Antibodies

AB262879

Anti-ZRANB1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZRANB1 antibody (AB262879)

  • 0
  • 0
Primary Antibodies

AB89290

Anti-TRIM26 antibody

Western blot - Anti-TRIM26 antibody (AB89290)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com