Mouse Polyclonal ORC6L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ORC6.
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- Cell death & disease 9:10352018Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKui Zhang et. al.PubMed 30305611
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ORC6. Database link Q9Y5N6
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3 µg/mL | Notes Antigen retrieval is not essential but may optimise staining. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Target data
Function
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Does not bind histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Alternative names
ORC6L, ORC6, Origin recognition complex subunit 6
Recommended products
Mouse Polyclonal ORC6L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ORC6.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ORC6. Database link Q9Y5N6
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ORC6L also known as Origin Recognition Complex Subunit 6 is a protein that plays a mechanical role in the initiation of DNA replication. It weighs approximately 30 kDa and localizes in the nucleoplasm of cells. ORC6L is expressed in various tissues with higher expression observed in testis pancreas and thymus. It is an important component ensuring the proper functioning and regulation of DNA replication origins.
- Biological function summary
DNA replication relies on ORC6L as part of the origin recognition complex (ORC) a multi-protein assembly essential for marking the origins of replication. The ORC complex of which ORC6L is a vital member initiates the assembly of pre-replicative complexes important for cell cycle progression. By binding to specific DNA sites ORC6L helps recruit other initiation factors ensuring that DNA replication occurs only once per cell cycle maintaining genomic stability.
- Pathways
Different phases of the cell cycle require ORC6L's role in the DNA replication pathway. This protein's function connects with other components of the ORC such as ORC1 ORC2 and ORC3 to control replication initiation. Furthermore ORC6L interacts with proteins such as CDC6 and MCM integrating into the DNA replication licensing pathway which ensures the cell's readiness for DNA synthesis and subsequent divisions.
- Associated diseases and disorders
Mutations or dysregulation of ORC6L have implications for genomic instability and diseases like Meier-Gorlin syndrome. This condition characterized by growth retardation and skeletal anomalies often links to other ORC components including ORC1 and ORC4 illustrating its involvement in developmental abnormalities. Additionally cancer research indicates the possible connection between ORC6L dysfunction and tumorigenesis due to unchecked cell proliferation emphasizing its importance in maintaining a balanced cell division process.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-ORC6L antibody (ab88686)
All lanes: Western blot - Anti-ORC6L antibody (ab88686) at 1 µg/mL
All lanes: Human pancreas tissue lysate at 50 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa
Western blot - Anti-ORC6L antibody (ab88686)
All lanes: Western blot - Anti-ORC6L antibody (ab88686) at 1 µg/mL
Lane 1: cell lysate from ORC6L transfected 293T cells at 25 µg
Lane 2: cell lysate from non transfected 293T cells at 25 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC6L antibody (ab88686)
ab88686 at 3 μg/ml detecting ORC6L in formalin-fixed paraffin-embedded Human placenta by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-ORC6L antibody (ab88686)
ab88686 at 10 μg/ml detecting ORC6L in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.
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