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AB88686

Anti-ORC6L antibody

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(1 Publication)

Mouse Polyclonal ORC6L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ORC6.

View Alternative Names

ORC6L, ORC6, Origin recognition complex subunit 6

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ORC6L antibody (AB88686)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ORC6L antibody (AB88686)

ab88686 at 10 μg/ml detecting ORC6L in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC6L antibody (AB88686)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORC6L antibody (AB88686)

ab88686 at 3 μg/ml detecting ORC6L in formalin-fixed paraffin-embedded Human placenta by immunohistochemistry.

Western blot - Anti-ORC6L antibody (AB88686)
  • WB

Unknown

Western blot - Anti-ORC6L antibody (AB88686)

All lanes:

Western blot - Anti-ORC6L antibody (ab88686) at 1 µg/mL

All lanes:

Human pancreas tissue lysate at 50 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Western blot - Anti-ORC6L antibody (AB88686)
  • WB

Unknown

Western blot - Anti-ORC6L antibody (AB88686)

All lanes:

Western blot - Anti-ORC6L antibody (ab88686) at 1 µg/mL

Lane 1:

cell lysate from ORC6L transfected 293T cells at 25 µg

Lane 2:

cell lysate from non transfected 293T cells at 25 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ORC6.

Q9Y5N6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORC6L also known as Origin Recognition Complex Subunit 6 is a protein that plays a mechanical role in the initiation of DNA replication. It weighs approximately 30 kDa and localizes in the nucleoplasm of cells. ORC6L is expressed in various tissues with higher expression observed in testis pancreas and thymus. It is an important component ensuring the proper functioning and regulation of DNA replication origins.
Biological function summary

DNA replication relies on ORC6L as part of the origin recognition complex (ORC) a multi-protein assembly essential for marking the origins of replication. The ORC complex of which ORC6L is a vital member initiates the assembly of pre-replicative complexes important for cell cycle progression. By binding to specific DNA sites ORC6L helps recruit other initiation factors ensuring that DNA replication occurs only once per cell cycle maintaining genomic stability.

Pathways

Different phases of the cell cycle require ORC6L's role in the DNA replication pathway. This protein's function connects with other components of the ORC such as ORC1 ORC2 and ORC3 to control replication initiation. Furthermore ORC6L interacts with proteins such as CDC6 and MCM integrating into the DNA replication licensing pathway which ensures the cell's readiness for DNA synthesis and subsequent divisions.

Mutations or dysregulation of ORC6L have implications for genomic instability and diseases like Meier-Gorlin syndrome. This condition characterized by growth retardation and skeletal anomalies often links to other ORC components including ORC1 and ORC4 illustrating its involvement in developmental abnormalities. Additionally cancer research indicates the possible connection between ORC6L dysfunction and tumorigenesis due to unchecked cell proliferation emphasizing its importance in maintaining a balanced cell division process.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Does not bind histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
See full target information ORC6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 9:1035 PubMed30305611

2018

Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Zhang,Gang Fu,Guangzhao Pan,Chongyang Li,Li Shen,Renjian Hu,Shunqin Zhu,Yibiao Chen,Hongjuan Cui
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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