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AB6214

Anti-Orexin A antibody

5

(2 Reviews)

|

(15 Publications)

Anti-Orexin A antibody (ab6214) is a rabbit polyclonal antibody detecting Orexin A in IHC-P, IHC-Fr, ELISA. Suitable for Cow, Mouse, Rat.

- Over 10 publications
- Trusted since 2000

View Alternative Names

OX, PPOX, HCRT, Hypocretin neuropeptide precursor, Hypocretin, Orexin precursor, Prepro-orexin, Preprohypocretin, Hcrt

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (AB6214)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (AB6214)

Immunohistochemical staining of orexin A in rat lateral hypothalamus useing Anti-Orexin A antibody (ab6214) at 1 : 50000 dilution. The primary antibody was detected with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (AB6214)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (AB6214)

Immunohistochemical staining of orexin A in rat zona incerta neurons using Rabbit antibody to Orexin A (ab6214) at a dilution of 1 in 1000.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Mouse, Cow

Applications

ELISA, IHC-FoFr, IHC-Fr, IHC-P

applications

Immunogen

Synthetic Peptide within Cow HCRT aa 1 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P56717

Specificity

Immunohistochemical analysis of rat brain indicates a good specificity for this serum.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Orexin A antibody (ab6214) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Cow, Mouse, Rat samples.

Trusted by the scientific community
Anti-Orexin A (ab6214) was first used in a scientific publication in 2000 and has been cited over 10 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 7 - 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Add glycerol to a final volume of 50% for extra stability and aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orexin A also known as orexin-a peptide or orexin a is a neuropeptide involved in various physiological processes. It has a molecular mass of approximately 3.5 kDa and consists of 33 amino acids. Orexin A is mainly produced in the lateral hypothalamus. It has a significant presence in the central nervous system where it binds to orexin receptors to stimulate neuronal activity. Despite being small it plays an important role in modulating multiple physiological functions across different systems in the body.
Biological function summary

Orexin A influences sleep-wake cycles and energy homeostasis. It acts independently not as part of a larger protein complex. It promotes wakefulness and regulates feeding behavior by activating orexin receptors which are G-protein-coupled receptors. These receptors are widely distributed throughout the brain indicating orexin A's role in maintaining alertness and energy expenditure. Furthermore it has interactions with several neuropeptides and neurotransmitters affecting reward systems and stress responses.

Pathways

The involvement of orexin A spans the sleep-wake regulation and energy metabolism pathways. This neuropeptide's function in the sleep-wake cycle involves interaction with neurotransmitters like norepinephrine and dopamine which enhance arousal. In energy metabolism it collaborates with proteins such as leptin and ghrelin influencing appetite and feeding behavior. Orexin A's ability to activate these pathways is important for the maintenance of energy balance and vigilance.

Orexin A has strong connections to narcolepsy and metabolic disorders such as obesity. In narcolepsy a deficiency or lack of orexin neurons leads to disrupted sleep-wake cycles resulting in excessive daytime sleepiness. Orexin antagonist drugs have been studied for their potential in treating insomnia by modulating its pathway. In metabolic disorders orexin A’s regulatory function on appetite links it to obesity where an imbalance in orexin signaling might affect energy expenditure. It interacts with leptin and insulin indicating its pivotal role in maintaining metabolic homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.. Orexin-A. Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).. Orexin-B. Binds to orexin receptor HCRTR2/OX2R only (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).
See full target information HCRT
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70606 PubMed40916543

2025

Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yao,Bingkai Ren,Qianhui Zhou,Yang Bai,Zhen Feng

Nature communications 15:5979 PubMed39013907

2024

Astrocytic metabolic control of orexinergic activity in the lateral hypothalamus regulates sleep and wake architecture.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Braga,Martina Chiacchiaretta,Luc Pellerin,Dong Kong,Philip G Haydon

Physiology & behavior 269:114289 PubMed37422081

2023

Metabolic dysfunctions following chronic oral corticosterone are modified by adolescence and sex in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ziasmin Shahanoor,Razia Sultana,Marina Savenkova,Ilia N Karatsoreos,Russell D Romeo

Communications biology 6:74 PubMed36658362

2023

Optogenetic and pharmacological interventions link hypocretin neurons to impulsivity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Susan M Tyree,Kimberly J Jennings,Oscar C Gonzalez,Shi-Bin Li,Janet R Nicholson,Moritz von Heimendahl,Luis de Lecea

Development (Cambridge, England) 149: PubMed35245348

2022

Selective requirement for polycomb repressor complex 2 in the generation of specific hypothalamic neuronal subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Behzad Yaghmaeian Salmani,Brad Balderson,Susanne Bauer,Helen Ekman,Annika Starkenberg,Thomas Perlmann,Michael Piper,Mikael Bodén,Stefan Thor

Brain structure & function 227:1051-1065 PubMed35066609

2022

Orexin A peptidergic system: comparative sleep behavior, morphology and population in brains between wild type and Alzheimer's disease mice.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhao,Yaqian You,Zhe Wang,Yanjun Zhou,Gaoshang Chai,Gen Yan,Zhewu Jin,Qing Wang,Hongxu Sun

ACS applied materials & interfaces 14:5066-5079 PubMed35041392

2022

Specific Silencing of Microglial Gene Expression in the Rat Brain by Nanoparticle-Based Small Interfering RNA Delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Guo,Fernando Cázarez-Márquez,Han Jiao,Ewout Foppen,Nikita L Korpel,Anita E Grootemaat,Nalan Liv,Yuanqing Gao,Nicole van der Wel,Bing Zhou,Guangjun Nie,Chun-Xia Yi

Scientific reports 11:21050 PubMed34702886

2021

Hypothalamic kinin B1 receptor mediates orexin system hyperactivity in neurogenic hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Rohan Umesh Parekh,Acacia White,Korin E Leffler,Vinicia C Biancardi,Jeffrey B Eells,Abdel A Abdel-Rahman,Srinivas Sriramula

Neurobiology of disease 134:104615 PubMed31605778

2019

Hypothalamic orexin and mechanistic target of rapamycin activation mediate sleep dysfunction in a mouse model of tuberous sclerosis complex.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Dongjun Guo,Lirong Han,Nicholas Rensing,Akiko Satoh,Michael Wong

Scientific reports 9:11146 PubMed31366942

2019

Considerations related to the use of short neuropeptide promoters in viral vectors targeting hypothalamic neurons.

Applications

Unspecified application

Species

Unspecified reactive species

N Kakava-Georgiadou,C Bullich-Vilarrubias,M M Zwartkruis,M C M Luijendijk,K M Garner,R A H Adan
View all publications
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