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AB89886

Anti-Orexin A antibody [MM0500-8G22]

5

(1 Review)

|

(1 Publication)

Mouse Monoclonal Orexin antibody. Suitable for IHC-Fr and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HCRT aa 1-100.

View Alternative Names

OX, PPORX, PPOX, HCRT, Hypocretin neuropeptide precursor, Hypocretin, Orexin precursor, Prepro-orexin, Preprohypocretin, Hcrt

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0500-8G22

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human HCRT aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O43612

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Alternative Products

Primary Antibodies

AB6214

Anti-Orexin A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (AB6214)

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  • 2
Primary Antibodies

AB35337

Anti-Orexin A antibody

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  • 1
Primary Antibodies

AB255294

Anti-Orexin A antibody [EPR22803-259]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (AB255294)

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Primary Antibodies

AB256507

Anti-Orexin A antibody [EPR22803-259] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] - BSA and Azide free (AB256507)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB183072

Anti-Orexin Receptor 2 antibody

Western blot - Anti-Orexin Receptor 2 antibody (AB183072)

  • 0
  • 0
Primary Antibodies

AB224368

Anti-Orexin Receptor 1/Ox-1-R antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin Receptor 1/Ox-1-R antibody - N-terminal (AB224368)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB134122

Anti-c-Fos antibody [EPR883(2)]

20ul selling size|KO Validated|Recombinant|RabMAb|Lab Essentials

Flow Cytometry (Intracellular) - Anti-c-Fos antibody [EPR883(2)] (AB134122)

  • 1
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orexin A also known as orexin-a peptide or orexin a is a neuropeptide involved in various physiological processes. It has a molecular mass of approximately 3.5 kDa and consists of 33 amino acids. Orexin A is mainly produced in the lateral hypothalamus. It has a significant presence in the central nervous system where it binds to orexin receptors to stimulate neuronal activity. Despite being small it plays an important role in modulating multiple physiological functions across different systems in the body.
Biological function summary

Orexin A influences sleep-wake cycles and energy homeostasis. It acts independently not as part of a larger protein complex. It promotes wakefulness and regulates feeding behavior by activating orexin receptors which are G-protein-coupled receptors. These receptors are widely distributed throughout the brain indicating orexin A's role in maintaining alertness and energy expenditure. Furthermore it has interactions with several neuropeptides and neurotransmitters affecting reward systems and stress responses.

Pathways

The involvement of orexin A spans the sleep-wake regulation and energy metabolism pathways. This neuropeptide's function in the sleep-wake cycle involves interaction with neurotransmitters like norepinephrine and dopamine which enhance arousal. In energy metabolism it collaborates with proteins such as leptin and ghrelin influencing appetite and feeding behavior. Orexin A's ability to activate these pathways is important for the maintenance of energy balance and vigilance.

Orexin A has strong connections to narcolepsy and metabolic disorders such as obesity. In narcolepsy a deficiency or lack of orexin neurons leads to disrupted sleep-wake cycles resulting in excessive daytime sleepiness. Orexin antagonist drugs have been studied for their potential in treating insomnia by modulating its pathway. In metabolic disorders orexin A’s regulatory function on appetite links it to obesity where an imbalance in orexin signaling might affect energy expenditure. It interacts with leptin and insulin indicating its pivotal role in maintaining metabolic homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.. Orexin-A. Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).. Orexin-B. Binds to orexin receptor HCRTR2/OX2R only (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).
See full target information HCRT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 162:974-86 PubMed26317466

2015

Inhibiting DNA Methylation Causes an Interferon Response in Cancer via dsRNA Including Endogenous Retroviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine B Chiappinelli,Pamela L Strissel,Alexis Desrichard,Huili Li,Christine Henke,Benjamin Akman,Alexander Hein,Neal S Rote,Leslie M Cope,Alexandra Snyder,Vladimir Makarov,Sadna Budhu,Sadna Buhu,Dennis J Slamon,Jedd D Wolchok,Drew M Pardoll,Matthias W Beckmann,Cynthia A Zahnow,Taha Merghoub,Taha Mergoub,Timothy A Chan,Stephen B Baylin,Reiner Strick
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Product promise

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