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AB199275

Anti-Orexin antibody

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(1 Publication)

Rabbit Polyclonal Orexin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HCRT aa 1-50.

View Alternative Names

OX, PPORX, PPOX, HCRT, Hypocretin neuropeptide precursor, Hypocretin, Orexin precursor, Prepro-orexin, Preprohypocretin, Hcrt

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin antibody (AB199275)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin antibody (AB199275)

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue labeling Pan Orexin with ab199275 at 1/20 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human HCRT aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O43612

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orexin also known as hypocretin is a neuropeptide that regulates arousal wakefulness and appetite. It comprises two types orexin-A and orexin-B and has a mass of approximately 3.5 kDa. Orexin is primarily produced in the hypothalamus but its receptors are found widely across the brain particularly in areas involved in regulating sleep and feeding behaviors. Orexin neurons release neurotransmitters and project to multiple brain regions influencing various physiological processes.
Biological function summary

Orexin functions as an important player in maintaining wakefulness and energy homeostasis. It does not act alone but interacts with receptors forming part of complex neural networks. The orexin system stimulates neurons that release other excitatory neurotransmitters therefore enhancing wakefulness and alertness. Through its action on the autonomic nervous system orexin impacts metabolic rate and can drive food intake when energy levels are low.

Pathways

Orexin integrates into the sleep-wake regulatory network and energy balance pathways. It is involved in the sleep-wakefulness cycle by modulating the levels of neurotransmitters like noradrenaline and dopamine. Orexin interacts with the hypothalamic-pituitary-adrenal axis impacting stress responses and energy expenditure. The involvement in these pathways links it to other proteins like leptin and ghrelin which also participate in energy homeostasis.

Orexin has significant implications in the pathophysiology of narcolepsy and obesity. Narcolepsy often results from the loss of orexin-producing neurons leading to excessive daytime sleepiness and sudden muscle weakness. Orexin's involvement in energy regulation connects it to obesity where orexin deficiency or altered signaling may contribute to weight gain and metabolic issues. In the context of these disorders orexin's interactions with leptin become significant as leptin influences appetite and energy expenditure highlighting a connection within the regulatory network affecting both sleep and metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.. Orexin-A. Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).. Orexin-B. Binds to orexin receptor HCRTR2/OX2R only (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).
See full target information HCRT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 22:288-97 PubMed26878232

2016

Rps14 haploinsufficiency causes a block in erythroid differentiation mediated by S100A8 and S100A9.

Applications

Unspecified application

Species

Unspecified reactive species

Rebekka K Schneider,Monica Schenone,Monica Ventura Ferreira,Rafael Kramann,Cailin E Joyce,Christina Hartigan,Fabian Beier,Tim H Brümmendorf,Ulrich Germing,Uwe Platzbecker,Guntram Büsche,Ruth Knüchel,Michelle C Chen,Christopher S Waters,Edwin Chen,Lisa P Chu,Carl D Novina,R Coleman Lindsley,Steven A Carr,Benjamin L Ebert
View all publications
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