AB281156

Anti-Orexin antibody [EPR22803-259] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Orexin antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.

View Alternative Names

Ox, Ppox, Hcrt, Hypocretin neuropeptide precursor, Hypocretin, Orexin precursor, Prepro-orexin, Preprohypocretin

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Sandwich ELISA - Anti-Orexin antibody [EPR22803-259] - BSA and Azide free (Capture) (AB281156)

sELISA

Lab

Sandwich ELISA - Anti-Orexin antibody [EPR22803-259] - BSA and Azide free (Capture) (AB281156)

Sandwich ELISA of ab253596 with the capture antibody ab281156 dilution at 2 µg/mL and detector antibody ab281006 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22803-259

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab281156 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse HCRT. The recommended pair for sandwich ELISA is:
Capture: ab281156, Mouse HCRT Capture Antibody (unconjugated)
Detector: ab281006, Mouse HCRT Detector Antibody (unconjugated)
The reference range value is 19.53-2500 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orexin also known as hypocretin is a neuropeptide that regulates arousal wakefulness and appetite. It comprises two types orexin-A and orexin-B and has a mass of approximately 3.5 kDa. Orexin is primarily produced in the hypothalamus but its receptors are found widely across the brain particularly in areas involved in regulating sleep and feeding behaviors. Orexin neurons release neurotransmitters and project to multiple brain regions influencing various physiological processes.

Biological function summary

Orexin functions as an important player in maintaining wakefulness and energy homeostasis. It does not act alone but interacts with receptors forming part of complex neural networks. The orexin system stimulates neurons that release other excitatory neurotransmitters therefore enhancing wakefulness and alertness. Through its action on the autonomic nervous system orexin impacts metabolic rate and can drive food intake when energy levels are low.

Pathways

Orexin integrates into the sleep-wake regulatory network and energy balance pathways. It is involved in the sleep-wakefulness cycle by modulating the levels of neurotransmitters like noradrenaline and dopamine. Orexin interacts with the hypothalamic-pituitary-adrenal axis impacting stress responses and energy expenditure. The involvement in these pathways links it to other proteins like leptin and ghrelin which also participate in energy homeostasis.

Orexin has significant implications in the pathophysiology of narcolepsy and obesity. Narcolepsy often results from the loss of orexin-producing neurons leading to excessive daytime sleepiness and sudden muscle weakness. Orexin's involvement in energy regulation connects it to obesity where orexin deficiency or altered signaling may contribute to weight gain and metabolic issues. In the context of these disorders orexin's interactions with leptin become significant as leptin influences appetite and energy expenditure highlighting a connection within the regulatory network affecting both sleep and metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.. Orexin-A. Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).. Orexin-B. Binds to orexin receptor HCRTR2/OX2R only (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).

See full target information Hcrt

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Orexin antibody [EPR22803-259] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-Orexin antibody [EPR22803-259] - BSA and Azide free (Capture) (AB281156)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise