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AB89888

Anti-Orexin B antibody [MM0501-5G21]

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(1 Publication)

Mouse Monoclonal Orexin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HCRT aa 50-100.

View Alternative Names

OX, PPORX, PPOX, HCRT, Hypocretin neuropeptide precursor, Hypocretin, Orexin precursor, Prepro-orexin, Preprohypocretin, Hcrt

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [MM0501-5G21] (AB89888)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin B antibody [MM0501-5G21] (AB89888)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4μm) labelling Orexin B with ab89888. Tissue was treated with 15μg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0501-5G21

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HCRT aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O43612

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Alternative Products

Primary Antibodies

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Primary Antibodies

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Complementary Products

Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Anti-c-Fos antibody [EPR883(2)]

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Flow Cytometry (Intracellular) - Anti-c-Fos antibody [EPR883(2)] (AB134122)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orexin B also known as hypocretin 2 is a neuropeptide with a molecular mass of about 2 kDa. It is one of the two types of orexins the other being orexin A. Orexin B is produced in the lateral hypothalamus and is found mainly in the brain. The neuropeptide binds to the orexin receptor type 2 (HCRTR2) to initiate its actions. The lateral hypothalamus synthesizes orexin B and then it is distributed broadly across neurological circuits. Orexin B plays an important role in regulating wakefulness and food intake by interacting with its receptor.
Biological function summary

Orexin B influences wakefulness energy metabolism and appetite regulation. It does not function alone but as part of the broader orexin neuropeptide system which includes orexin A and their respective receptors forming a regulatory network. The actions of orexin B impact the arousal system effectively enhancing alertness when appropriately activated. The neuropeptide's modulation of the sleep-wake cycle and feeding behaviors indicates its relevance in maintaining critical homeostatic circuits within the central nervous system.

Pathways

Orexin B is significantly involved in the sleep-wake cycle and feeding regulatory pathways. Within these pathways orexin B interacts closely with neurotransmitters and receptors like norepinephrine and the HCRTR2 receptor. This interaction supports its role in maintaining wakefulness and energy balance. During activation orexin B influences the locus coeruleus an important site of noradrenergic neuron activity linking it to broader neurophysiological processes critical to arousal and stress response mechanisms.

Disruptions in orexin B activity lead to conditions such as narcolepsy and obesity. Narcolepsy a sleep disorder characterized by debilitating daytime drowsiness connects to changes in orexin signaling particularly those affecting orexin receptors and their interaction with orexin A. Orexin B's relation to obesity stems from its influence on food intake and energy balance bridging metabolic disorders and neuropathological conditions. Research into these connections emphasizes the therapeutic potential of targeting this neuropeptide system to manage sleep disorders and metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.. Orexin-A. Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).. Orexin-B. Binds to orexin receptor HCRTR2/OX2R only (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).
See full target information HCRT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:67 PubMed38429845

2024

Single-cell deconvolution algorithms analysis unveils autocrine IL11-mediated resistance to docetaxel in prostate cancer via activation of the JAK1/STAT4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bisheng Cheng,Lingfeng Li,Tianlong Luo,Qiong Wang,Yong Luo,Shoumin Bai,Kaiwen Li,Yiming Lai,Hai Huang
View all publications
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Product promise

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