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AB16584

Anti-Orexin Prepro antibody

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(1 Publication)

Rabbit Polyclonal Orexin antibody. Suitable for ELISA, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Ox, Ppox, Hcrt, Hypocretin neuropeptide precursor, Hypocretin, Orexin precursor, Prepro-orexin, Preprohypocretin

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified by antigen affinity chromatography.
Storage buffer
pH: 7.4 Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The orexin prepro also known as prepro-orexin is a precursor molecule with a mass of approximately 16-18 kDa. It undergoes proteolytic cleavage to form orexin-A and orexin-B neuropeptides. These peptides play an essential role in maintaining wakefulness and energy homeostasis. The orexin prepro is mainly expressed in the hypothalamus particularly in the lateral hypothalamic area where it acts on orexin receptors distributed widely across the brain.
Biological function summary

The orexin neuropeptides derived from the orexin prepro are important for regulating sleep-wake cycles appetite and arousal. While not part of a larger complex orexins are significant modulators of other neurotransmitter systems including noradrenaline dopamine and serotonin. These interactions influence many physiological functions further demonstrating the importance of orexins in maintaining overall body balance.

Pathways

Orexin prepro-derived neuropeptides engage in the sleep-wake regulation and energy homeostasis pathways. They interact directly with orexin receptors such as OX1R and OX2R to affect these pathways. The insulin signaling pathway also involves orexin peptides linking them with metabolic regulation. Other proteins such as leptin and ghrelin interact with orexins indicating the overlap of neuronal and metabolic pathways in which orexins play.

Disruptions in orexin signaling have strong associations with narcolepsy and obesity. Narcolepsy characterized by fragmented sleep-wake cycles shows a loss of orexin-producing neurons. Obesity relates to orexin through its involvement in appetite regulation and energy balance with dysregulation leading to weight gain. Orexin peptides and their receptors are potential therapeutic targets for these conditions creating connections with other proteins like melanin-concentrating hormone involved in energy homeostasis and sleep regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.. Orexin-A. Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).. Orexin-B. Binds to orexin receptor HCRTR2/OX2R only (By similarity). Stimulates food intake (By similarity). Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner (By similarity).
See full target information Hcrt
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of steroid biochemistry and molecular 114:135-43 PubMed19429443

2009

Genome-wide analysis of DHEA- and DHT-induced gene expression in mouse hypothalamus and hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Qianxing Mo,Shifang Lu,Carrie Garippa,Michael J Brownstein,Neal G Simon
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