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AB77370

Anti-Orexin Receptor 1 and 2 antibody

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(4 Publications)

Goat Polyclonal Orexin Receptor 1/Ox-1-R antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Orexin/Hypocretin receptor type 1, Hypocretin receptor type 1, Orexin receptor type 1, Ox-1-R, Ox1-R, Ox1R, HCRTR1

1 Images
Western blot - Anti-Orexin Receptor 1 and 2 antibody (AB77370)
  • WB

Unknown

Western blot - Anti-Orexin Receptor 1 and 2 antibody (AB77370)

All lanes:

Western blot - Anti-Orexin Receptor 1 and 2 antibody (ab77370) at 0.5 µg/mL

All lanes:

human frontal cortex lysate in RIPA buffer at 35 µg

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orexin Receptor 1 and 2 also known as hypocretin receptor 1 and 2 are G-protein-coupled receptors that bind to neuropeptides orexin-A and orexin-B. Orexin Receptor 1 has a molecular mass of approximately 50-60 kDa while Orexin Receptor 2 is similar in size. These receptors are mainly expressed in the brain particularly in the hypothalamus and other regions regulating wakefulness and sleep. The binding of orexins to these receptors triggers intracellular signaling cascades important for various physiological functions.
Biological function summary

These receptors play a significant role in modulating sleep-wake cycles and energy homeostasis. Orexin Receptors do not form part of a larger complex but interact with other proteins within the cell signaling networks. They are involved in the regulation of feeding behavior arousal and reward systems. Activation of these receptors promotes wakefulness and increases energy expenditure affecting the central nervous system's balance.

Pathways

Orexin Receptors participate in the neuropeptide signaling pathway and the neuronal system pathway. They interact with G-proteins to mediate their signals linking them to secondary messenger systems like cyclic AMP. These receptors also engage with proteins such as GABA receptors and adenosine receptors modulating inhibitory and excitatory neurotransmitter release to maintain alertness and metabolic processes.

Orexin Receptors are closely associated with sleep disorders such as narcolepsy and insomnia. Narcolepsy often involves the loss of orexin-producing neurons leading to disruptions in normal sleep patterns. These receptors also have connections to metabolic disorders like obesity where dysregulation of orexin signaling can affect appetite and energy expenditure. The alteration of these signals further involves interactions with leptin and ghrelin highlighting the complex nature of energy balance and appetite regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide (PubMed : 26950369, PubMed : 9491897). Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding (PubMed : 26950369, PubMed : 9491897).
See full target information HCRTR1

Additional targets

HCRTR2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 14:1206353 PubMed37441503

2023

Chaihu-Longgu-Muli decoction improves sleep disorders by restoring orexin-A function in CKD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Li Cao,Xue-Mei Peng,Gong-Bo Li,Wei-Sen Ding,Kai-Zhen Wang,Xiao-Lei Wang,Yan-Ying Xiong,Wei-Jian Xiong,Fan Li,Min Song

Journal of biochemical and molecular toxicology 34:e22493 PubMed32319158

2020

Bilobalide assuages morphine-induced addiction in hippocampal neuron cells through upregulation of microRNA-101.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Hongyin Zhai

International journal of molecular medicine 43:435-442 PubMed30365112

2018

Amyloid β and tau are involved in sleep disorder in Alzheimer's disease by orexin A and adenosine A(1) receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Liu,Fumin Wang,Minglu Tang,Yongjun Zhao,Xiaoting Wang

mSphere 3: PubMed29976647

2018

Transporters MRP1 and MRP2 Regulate Opposing Inflammatory Signals To Control Transepithelial Neutrophil Migration during Streptococcus pneumoniae Lung Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Zukauskas,Randall J Mrsny,Paula Cortés Barrantes,Jerrold R Turner,John M Leong,Beth A McCormick
View all publications
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Product promise

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