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AB224368

Anti-Orexin Receptor 1/Ox-1-R antibody - N-terminal

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(2 Publications)

Rabbit Polyclonal Orexin Receptor 1/Ox-1-R antibody. N-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HCRTR1 aa 1-50.

View Alternative Names

ORXR1, OX1R, OXR1, HCRTR1, Orexin/Hypocretin receptor type 1, Hypocretin receptor type 1, Orexin receptor type 1, Ox-1-R, Ox1-R, Ox1R

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin Receptor 1/Ox-1-R antibody - N-terminal (AB224368)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin Receptor 1/Ox-1-R antibody - N-terminal (AB224368)

Paraffin-embedded human cerebellum tissue stained for Orexin Receptor 1/Ox-1-R using ab224368 at 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HCRTR1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O43613

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Complementary Products

Primary Antibodies

AB183072

Anti-Orexin Receptor 2 antibody

Western blot - Anti-Orexin Receptor 2 antibody (AB183072)

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Primary Antibodies

AB255294

Anti-Orexin A antibody [EPR22803-259]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (AB255294)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB15323

Anti-iNOS antibody

BOND RX™ Validated

Western blot - Anti-iNOS antibody (AB15323)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Orexin Receptor 1 also known as Ox-1-R or Ox protein is a G-protein-coupled receptor that binds to orexin A peptides. The receptor has a mass of approximately 50 kDa and is expressed in various brain regions including the hypothalamus. Its presence is notable in areas involved in arousal and regulation of sleep-wake cycles. Through binding to its ligands Ox-1-R activates intracellular signaling cascades by coupling with G-proteins contributing to the modulation of neurotransmission and neuronal excitability.
Biological function summary

Orexin receptors regulate energy homeostasis and alertness. Ox-1-R is involved in the control of wakefulness and appetite as it interacts with neurotransmitters such as noradrenaline and histamine. Ox-1-R does not function in isolation; it works in tandem with other receptors and proteins including orexin receptor 2 to form integrated networks for maintaining physiological balance. Their role in the central nervous system is essential for coordinating physical and mental activity.

Pathways

Orexin 1 receptor participates in the neuropeptide signaling pathways and sleep regulation mechanisms. Orexin receptors take part in the hypothalamic neuropeptide pathways interacting with other proteins like melanin-concentrating hormone and corticotropin-releasing factor facilitating the control of sleep and feeding cycles. These interactions underline the receptor’s regulatory functions on metabolic and circadian rhythm processes ensuring organismal adaptation to varying environmental cues.

Orexin 1 receptor dysfunction associates with sleep disorders such as narcolepsy and conditions like obesity. Reduced activity of Ox-1-R can contribute to narcolepsy a chronic neurological disorder characterized by excessive daytime sleepiness and cataplexy. In obesity dysregulation of the orexin signaling might influence energy balance and appetite linking it with metabolic disorders. Related proteins in these conditions include orexin receptor 2 and neuropeptide Y indicating a broader network of interactions affecting these disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor that binds the neuropeptide orexin-A with high affinity, and orexin-B with lower affinity, two peptides derived from a common precursor, prepro-orexin (PubMed : 32669442, PubMed : 9491897). Its activity is mediated via a G(q)-protein-coupled pathway, which activates the phosphatidylinositol-calcium second messenger system in response to orexin-A binding (PubMed : 32669442). In addition to G(q)-mediated signaling, orexin-A stimulation also promotes beta-arrestin recruitment, leading to receptor internalization (PubMed : 15683363, PubMed : 32669442). Plays a significant role in the regulation of food intake (By similarity).
See full target information HCRTR1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110537 PubMed39193188

2024

Time- and cell-specific activation of BMP signaling restrains chondrocyte hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen J Gadomski,Byron W H Mui,Raphael Gorodetsky,Sriram S Paravastu,Joseph Featherall,Li Li,Abigail Haffey,Jae-Chun Kim,Sergei A Kuznetsov,Kathryn Futrega,Astar Lazmi-Hailu,Randall K Merling,Daniel Martin,Andrew W McCaskie,Pamela G Robey

Aging 16:7946-7960 PubMed38713160

2024

Trigeminal nerve electrical stimulation attenuates early traumatic brain injury through the TLR4/NF-κB/NLRP3 signaling pathway mediated by orexin-A/OX1R system.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Kang,Yifan Zhou,Qi Xiong,Xiaoyang Dong
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Product promise

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