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AB183072

Anti-Orexin Receptor 2 antibody

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(6 Publications)

Rabbit Polyclonal Orexin Receptor 2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human HCRTR2 aa 400 to C-terminus.

View Alternative Names

Orexin receptor type 2, Ox-2-R, Ox2-R, Ox2R, Hypocretin receptor type 2, HCRTR2

1 Images
Western blot - Anti-Orexin Receptor 2 antibody (AB183072)
  • WB

Supplier Data

Western blot - Anti-Orexin Receptor 2 antibody (AB183072)

All lanes:

Western blot - Anti-Orexin Receptor 2 antibody (ab183072) at 1/1100 dilution

Lane 1:

mouse kidney tissue lysate at 40 µg

Lane 2:

mouse brain tissue lysate at 40 µg

Lane 3:

mouse heart tissue lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution

Predicted band size: 50 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human HCRTR2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43614

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Orexin Receptor 2 also known as Orexin 2 receptor or hypocretin receptor 2 is a G-protein coupled receptor (GPCR) encoded by the HCRTR2 gene. This receptor has a molecular weight of approximately 50 kDa. Expressed mainly in the brain including the hypothalamus hippocampus and certain cortical regions the receptor interacts with the neuropeptides orexin A and orexin B. The receptor activation triggers intracellular signaling cascades involving the Gq and Gi proteins which modulate neural activity.
Biological function summary

The Orexin Receptor 2 plays a critical role in maintaining wakefulness and energy metabolism. This receptor does not function as part of a larger protein complex but its interactions with the orexins influence diverse physiological responses like feeding behavior arousal and sleep-wake cycles. Orexin GPCRs including Orexin Receptor 2 have distinct but overlapping roles in these processes.

Pathways

Orexin Receptor 2 integrates into the neural circuits regulating the sleep-wake cycle and energy homeostasis. It participates in the orexin signaling pathway influencing neurotransmitter release that affects arousal and appetite. Additionally the receptor links with proteins such as orexin itself and other neurotransmitters which modulate sleep and arousal states through interconnected pathways including the limbic system.

Orexin Receptor 2 is closely linked to conditions such as narcolepsy and insomnia. Narcolepsy characterized by excessive daytime sleepiness and sudden loss of muscle tone often involves disrupted orexin signaling due to low orexin levels or receptor dysfunction. Furthermore the receptor targets have been explored in the context of insomnia treatments focusing on their ability to regulate sleep patterns. Altered orexin signaling involving the Orexin 2 protein and other related neurotransmitters can impact both conditions showing the receptor’s clinical significance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides (PubMed : 26950369, PubMed : 9491897). Triggers an increase in cytoplasmic Ca(2+) levels in response to orexin-A binding (PubMed : 26950369, PubMed : 9491897).
See full target information HCRTR2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Brain : a journal of neurology 147:4280-4291 PubMed38829801

2024

Nociceptors are functionally male or female: from mouse to monkey to man.

Applications

Unspecified application

Species

Unspecified reactive species

Harrison Stratton,Grace Lee,Mahdi Dolatyari,Andre Ghetti,Tamara Cotta,Stefanie Mitchell,Xu Yue,Mohab Ibrahim,Nicolas Dumaire,Lyuba Salih,Aubin Moutal,Liberty François-Moutal,Laurent Martin,Edita Navratilova,Frank Porreca

Journal of neuroinflammation 21:131 PubMed38760784

2024

Therapeutic effects of orexin-A in sepsis-associated encephalopathy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Guo,Zhuo Kong,Sha Yang,Jingjing Da,Liangzhao Chu,Guoqiang Han,Jian Liu,Ying Tan,Jiqin Zhang

iScience 27:109067 PubMed38361621

2024

An orexin-receptor-2-mediated heart-brain axis in cardiac pain.

Applications

Unspecified application

Species

Unspecified reactive species

Han Jiao,Yongjin Wang,Kang Fu,Xiaoao Xiao,Mo-Qiu Jia,Jia Sun,Jingxiao Wang,Guoqing Zhu,Daying Lyu,Qiulun Lu,Yu Peng,Juan Lv,Li Su,Yuanqing Gao

Frontiers in molecular neuroscience 15:1013182 PubMed36277486

2022

Plasticity of the hypocretinergic/orexinergic system after a chronic treatment with suvorexant in rats. Role of the hypocretinergic/orexinergic receptor 1 as an autoreceptor.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Carrera-Cañas,Isabel de Andrés,Marta Callejo,Miguel Garzón

Medical science monitor : international medical jo 25:2886-2895 PubMed31002658

2019

Effects of Sleep Deprivation (SD) on Rats via ERK1/2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Youyi Gu,Jingjing Zhang,Li Gong

Frontiers in neuroscience 13:282 PubMed30971886

2019

Orexin Receptor-1 in the Rostral Ventrolateral Medulla Mediates the Antihypertensive Effects of Electroacupuncture.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ying Tan,Ling Fang,Fan-Rong Yao,Dong-Yuan Cao,Qi Zhang
View all publications
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