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AB273435

Anti-SARS-CoV-2 orf7a antibody [3C9]

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Mouse Monoclonal NS7A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with SARS-CoV-2 samples.

View Alternative Names

ORF7a protein, Accessory protein 7a, Protein U122, Protein X4, 7a, Protein X4, Protein U122, Accessory protein 7a

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

Immunofluorescence analysis of Vero E6 (african green monkey kidney epithelial cell line) cells labelling Protein 7a (green) using ab273435. Red : MitoTracker. Left Panel is merged images.

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)
  • WB

Lab

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

False colour image of Western blot : Anti-ORF7a Protein antibody [3C9] - Coronavirus staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab273435 was shown to bind specifically to ORF7a Protein. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (ab273435) at 1/1000 dilution

All lanes:

Recombinant SARS-CoV2 ORF7a Fc chimera protein cell lysate at 0.5 µg

Observed band size: 40 kDa

false

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

All lanes:

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (ab273435) at 1/500 dilution

Lane 1:

SARS-CoV-infected Vero E6 (african green monkey kidney epithelial cell line) whole cell lysate

Lane 2:

8 hours after SARS-CoV-infected Vero E6 whole cell lysate

Lane 3:

16 hours after SARS-CoV-infected Vero E6 whole cell lysate

Lane 4:

24 hours after SARS-CoV-infected Vero E6 whole cell lysate

false

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)
  • WB

Supplier Data

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (AB273435)

All lanes:

Western blot - Anti-SARS-CoV-2 orf7a antibody [3C9] (ab273435) at 1/1000 dilution

Lane 1:

Mock, Caco2 (human colorectal adenocarcinoma cell line) whole cell lysate

Lane 2:

SARS-CoV infected, Caco2 whole cell lysate

Lane 3:

SARS-CoV-2, Caco2 whole cell lysate

false

  • Unconjugated

    Anti-ATP-binding cassette sub-family A member 3 antibody [3C9]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3C9

Isotype

IgG1

Carrier free

Yes

Reacts with

SARS-CoV-2

Applications

ICC/IF, WB

applications

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ORF7a protein also known as accessory protein 7a acts as an important virulence factor in SARS-CoV-2 the virus causing COVID-19. The protein has a molecular mass of approximately 12 kDa and is expressed in the endoplasmic reticulum. It extends into the lumen where it can bind to proteins and other molecules. ORF7a is known to interact with different host proteins manipulating cellular machinery to aid viral replication and pathogenesis.
Biological function summary

Functions of the ORF7a protein extend to immune system inhibition. It can interfere with type I interferon signaling reducing the host's antiviral response. As part of viral assembly ORF7a contributes to the efficient packaging and release of viral particles. It does not form a complex with other viral proteins but acts independently to execute its functions within the infected host cell.

Pathways

Roles of the ORF7a protein integrate into immune escape and virion production processes. It participates in immune evasion pathways by inhibiting signaling pathways that interfere with interferon production like the JAK-STAT pathway. Additionally it has associations with other SARS-CoV-2 proteins like ORF3b which jointly compromise host cell functions and enhance virus survival.

The ORF7a protein has strong connections to the manifestation of COVID-19-related symptoms. The inhibition of interferon signaling contributes to respiratory distress observed in severe cases of COVID-19. Studies also indicate links to the inflammatory responses involving other viral proteins such as the N protein which together can exacerbate the severity of SARS-CoV-2 infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role as antagonist of host tetherin (BST2), disrupting its antiviral effect. Acts by binding to BST2 thereby interfering with its glycosylation. May suppress small interfering RNA (siRNA). May bind to host ITGAL, thereby playing a role in attachment or modulation of leukocytes.
See full target information 7a
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Product promise

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