Lysosome and autophagosome markers

Investigate the mechanics of cellular turnover with our validated markers for lysosomes and autophagosomes, including LC3, p62, and LAMP1. These high-performance antibodies are part of our range of over 60k primary antibodies, and are supported by over 600k citations. They provide the spatial resolution and sensitivity needed to monitor autophagy flux and protein degradation with total clarity.

Lysosomes

What is a lysosome?

Lysosomes are membrane-bound organelles essential for cellular waste processing and recycling. LAMP1 and LAMP2 (Lysosome-Associated Membrane Proteins 1 and 2) are widely used markers for the lysosomal membrane, often targeted in immunofluorescence and cell imaging to visualize lysosomal distribution and abundance. A key player in lysosomal enzyme trafficking is the mannose-6-phosphate receptor (M6PR), which resides primarily in the trans-Golgi network and endosomes. M6PR recognizes lysosomal enzymes tagged with mannose-6-phosphate and directs them to lysosomes, ensuring proper delivery of hydrolases.

What are Lysosome Markers used to study?

Lysosomes operate within a membrane network involving the ER and Golgi apparatus, receiving cargo for degradation and recycling. Marker-based identification is crucial for studying lysosomal function, autophagy, and lysosome-related disorders.

High-quality lysosome markers

abcam:product-category/primary-antibodies

ab108597

Anti-LAMP1 antibody [EPR4204] - Lysosome Marker

Validated for western blot and ICC/IF
KO validated for confirmed specificity
Over 10 publications

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abcam:product-category/primary-antibodies

ab125068

Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

Validated for western blot, flow cytometry (intra), flow cytometry, IP, IHC-P, ICC/IF
KO validated for confirmed specificity
Over 50 publications

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abcam:product-category/primary-antibodies

ab124767

Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker

Validated for western blot, flow cytometry (intra), flow cytometry, IHC-P, ICC/IF
KO validated for confirmed specificity
Over 70 publications

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Autophagosomes

What is an autophagosome?

Autophagosomes are double-membraned vesicles that play a central role in autophagy, a cellular degradation pathway that recycles cytoplasmic components. One of the most widely used markers is LC3 (microtubule-associated protein 1A/1B-light chain 3), particularly its lipidated form, LC3-II, which integrates into the autophagosomal membrane and is essential for autophagosome elongation. Another key marker is p62/SQSTM1, a cargo adaptor protein that links ubiquitinated substrates to LC3 and is degraded upon autophagy completion, making it a useful indicator of autophagic flux.