Rabbit Polyclonal ORMDL1 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ORMDL1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ORMDL1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0S3
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Target data
Function
Plays an essential role in the homeostatic regulation of sphingolipid de novo biosynthesis by modulating the activity of the serine palmitoyltransferase (SPT) in response to ceramide levels (PubMed:20182505). When complexed to SPT, the binding of ceramides to its N-terminus stabilizes a conformation that block SPT substrate entry, hence preventing SPT catalytic activity. Through this mechanism, maintains ceramide levels at sufficient concentrations for the production of complex sphingolipids, but which prevents the accumulation of ceramides to levels that trigger apoptosis (By similarity).
Alternative names
HSPC202, ORMDL1, ORM1-like protein 1, Adoplin-1
Recommended products
Rabbit Polyclonal ORMDL1 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ORMDL1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ORMDL1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P0S3
- Purification technique
- Affinity purification Immunogen
- Specificity
ab176122 may cross-react with other ORMDL protein family members.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ORMDL1 protein also known as ORM1-like protein 1 is a member of the ORMDL family playing significant roles in cellular processes. With a mass of approximately 18 kDa ORMDL1 is a transmembrane protein localized in the endoplasmic reticulum. Expression of ORMDL1 occurs across various tissues indicating its broad functional significance in different physiological contexts.
- Biological function summary
The main function of ORMDL1 involves regulation of sphingolipid biosynthesis. It forms a complex with serine palmitoyltransferase the enzyme responsible for the first step in sphingolipid synthesis. Through this interaction ORMDL1 modulates the synthesis rate of ceramides and other sphingolipids thereby influencing cell membrane structure and cellular signaling pathways. This regulatory mechanism is important for maintaining cellular homeostasis.
- Pathways
The sphingolipid biosynthesis pathway involves interactions with ORMDL1 impacting cellular stress responses and apoptosis. Another relevant pathway includes the endoplasmic reticulum stress response where ORMDL1 interacts with proteins like ATF6. These interactions suggest the role of ORMDL1 in modulating responses to cellular stress and promoting survival by maintaining lipid equilibrium.
- Associated diseases and disorders
ORMDL1 is linked to conditions like asthma and type 2 diabetes. In asthma altered sphingolipid metabolism has been associated with airway inflammation where ORMDL1 might interact with related proteins like SPTLC1 affecting the inflammatory response. In type 2 diabetes the dysregulation of lipid metabolism involving ORMDL1 could play part in insulin resistance highlighting the importance of ORMDL1 in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
Western blot - Anti-ORMDL1 antibody (ab176122)
Lane 1: Western blot - Anti-ORMDL1 antibody (ab176122) at 1 µg/mL
Lane 2: Western blot - Anti-ORMDL1 antibody (ab176122) at 2 µg/mL
All lanes: SK-N-SH cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 17 kDa
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