AB211522

Anti-ORMDL3 antibody

Name: Anti-ORMDL3 antibody (ab211522) | Abcam
Brand: Abcam Limited
SKU: AB211522

(1 Review)

(5 Publications)

Rabbit Polyclonal ORMDL3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ORMDL3 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ORM1-like protein 3, ORMDL3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORMDL3 antibody (AB211522)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORMDL3 antibody (AB211522)

Immunohistochemical analysis of Human spleen tissue labeling ORMDL3 with ab211522 at a 1/100 dilution. Heat mediated antigen retrieval used.

Supplier Data

Western blot - Anti-ORMDL3 antibody (AB211522)

All lanes:

Western blot - Anti-ORMDL3 antibody (ab211522) at 1/1000 dilution

All lanes:

Mouse testis whole cell lysate at 35 µg

Predicted band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ORMDL3 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8N138

Specificity

The immunogenic peptide shares 100% homology with family members ORMDL1 and ORMDL2. Cross reactivity with these family members has not been tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORMDL3 also known as ORM1-like protein 3 is a transmembrane protein located in the endoplasmic reticulum. The molecular mass of ORMDL3 is approximately 17 kDa. This protein is expressed ubiquitously meaning it is found in most tissues throughout the body. ORMDL3 plays an important role in regulating sphingolipid biosynthesis by inhibiting the serine palmitoyltransferase (SPT) complex which is the first step in the sphingolipid de novo pathway.

Biological function summary

ORMDL3 modulates lipid metabolism and immune response regulation. It does this by being part of a regulatory complex with the serine palmitoyltransferase therefore influencing the production of sphingolipids which are essential components of cell membranes and contribute to cell signaling. Alterations in sphingolipid levels can affect membrane integrity and cell communication impacting cellular function and health.

Pathways

ORMDL3 influences sphingolipid metabolic processes and endoplasmic reticulum stress responses. In the sphingolipid metabolism pathway it directly interacts with SPTLC1 and SPTLC2 core components of the serine palmitoyltransferase enzyme complex. Additionally ORMDL3 indirectly affects the unfolded protein response pathway by influencing levels of sphingolipids which play a role in stress signaling in the endoplasmic reticulum.

Studies link ORMDL3 to asthma and inflammatory bowel disease (IBD). ORMDL3's role in sphingolipid regulation affects inflammation and immune responses processes that are critical in the pathology of these diseases. For example genetic variations in ORMDL3 correlate with increased risk of asthma potentially through interactions with other immune-related proteins such as GSDMB. Changes in ORMDL3 expression levels can influence the severity and progression of inflammatory disorders by modulating the sphingolipid-mediated signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an essential role in the homeostatic regulation of sphingolipid de novo biosynthesis by modulating the activity of the serine palmitoyltransferase (SPT) in response to ceramide levels (PubMed : 20182505, PubMed : 30700557, PubMed : 37308477). When complexed to SPT, the binding of ceramides to its N-terminus stabilizes a conformation that block SPT substrate entry, hence preventing SPT catalytic activity. Through this mechanism, maintains ceramide levels at sufficient concentrations for the production of complex sphingolipids, but which prevents the accumulation of ceramides to levels that trigger apoptosis (PubMed : 37308477).

See full target information ORMDL3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Phenomics (Cham, Switzerland) 3:485-495 PubMed37881318

2023

A Common Functional Variant at the Enhancer of the Rheumatoid Arthritis Risk Gene Regulates its Expression Through Allele-Specific JunD Binding.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Ye,Yiyun Yu,Xiaoxia Zhu,Weiguo Wan,Yun Liu,Hejian Zou,Zaihua Zhu

Molecular medicine reports 28: PubMed37594074

2023

ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Liu,Yan Sun,Yun Zhou,Yuye Gao,Qijun Song,Jianmei Yang,Chao Xu,Guimei Li

IET nanobiotechnology 17:182-196 PubMed36680386

2023

The inhibition of ORMDL3 prevents Alzheimer's disease through ferroptosis by PERK/ATF4/HSPA5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yankun Shao,Yilin Xu,Huang Di,Xinxiu Shi,Yingying Wang,Hongyu Liu,Lina Song

Medical oncology (Northwood, London, England) 39:159 PubMed35972600

2022

Regulation of the sensitivity of hepatocarcinoma cells by ORMDL3, to sorafenib by autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yixiao Sun,Xueran Guan,Ting Zhang,Yue Li,Huiling Shi,Ashleigh Tinotenda Chitakunye,Hanyu Hong,Shihui Zhang,Qin Zhu,Lin Cai

Cell death & disease 9:483 PubMed29706629

2018

Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenlu Li,Guangzhi Wang,Dongcheng Feng,Guo Zu,Yang Li,Xue Shi,Yan Zhao,Huirong Jing,Shili Ning,Weidong Le,Jihong Yao,Xiaofeng Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ORMDL3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORMDL3 antibody (AB211522)

Western blot - Anti-ORMDL3 antibody (AB211522)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise